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Ocular surface inflammation induces de novo expression of substance P in the trigeminal primary afferents with large cell bodies

We evaluated the changes in substance P (SP)-expressing trigeminal neurons (TNs) innervating the cornea following ocular surface inflammation. Ocular surface inflammation was induced in Sprague–Dawley rats using 0.1% benzalkonium chloride (BAK). The corneal staining score, corneal epithelial apoptos...

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Autores principales: Byun, Yong-Soo, Mok, Jee-Won, Chung, So-Hyang, Kim, Hyun-Seung, Joo, Choun-Ki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494893/
https://www.ncbi.nlm.nih.gov/pubmed/32939029
http://dx.doi.org/10.1038/s41598-020-72295-x
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author Byun, Yong-Soo
Mok, Jee-Won
Chung, So-Hyang
Kim, Hyun-Seung
Joo, Choun-Ki
author_facet Byun, Yong-Soo
Mok, Jee-Won
Chung, So-Hyang
Kim, Hyun-Seung
Joo, Choun-Ki
author_sort Byun, Yong-Soo
collection PubMed
description We evaluated the changes in substance P (SP)-expressing trigeminal neurons (TNs) innervating the cornea following ocular surface inflammation. Ocular surface inflammation was induced in Sprague–Dawley rats using 0.1% benzalkonium chloride (BAK). The corneal staining score, corneal epithelial apoptosis, conjunctival goblet cells, and density of corneal subbasal nerve plexus (SNP) were assessed, and the mRNA levels of SP, interleukin (IL)-1β, IL-6, and tumour necrosis factor-α were measured in corneas and ipsilateral trigeminal ganglia (TG). SP-immunoreactivity (IR) was measured in corneal intraepithelial nerves and TNs. The cell size of corneal TNs in the TG was calculated. All parameters were observed immediately (BAK group), at 1 week (1 w group), and 2 months (2 m group) after 2 weeks of BAK application. BAK caused an increase in the corneal staining score and the number of apoptotic cells, loss of conjunctival goblet cells, reduced density of corneal SNP, and upregulated expression of SP and inflammatory cytokines in both the cornea and TG in the BAK group but those changes were not observed in the 2 m group. On the other hand, SP-IR% and mean cell size of corneal TNs increased significantly in the BAK, 1 w, and 2 m groups, compared to the control. Our data suggest that following ocular surface inflammation, large-sized corneal TNs which normally do not express SP, expressed it and this phenotype switching lasted even after the inflammation disappeared. Long-lasting phenotypic switch, as well as changes in the expression level of certain molecules should be addressed in future studies on the mechanism of corneal neuropathic pain.
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spelling pubmed-74948932020-09-18 Ocular surface inflammation induces de novo expression of substance P in the trigeminal primary afferents with large cell bodies Byun, Yong-Soo Mok, Jee-Won Chung, So-Hyang Kim, Hyun-Seung Joo, Choun-Ki Sci Rep Article We evaluated the changes in substance P (SP)-expressing trigeminal neurons (TNs) innervating the cornea following ocular surface inflammation. Ocular surface inflammation was induced in Sprague–Dawley rats using 0.1% benzalkonium chloride (BAK). The corneal staining score, corneal epithelial apoptosis, conjunctival goblet cells, and density of corneal subbasal nerve plexus (SNP) were assessed, and the mRNA levels of SP, interleukin (IL)-1β, IL-6, and tumour necrosis factor-α were measured in corneas and ipsilateral trigeminal ganglia (TG). SP-immunoreactivity (IR) was measured in corneal intraepithelial nerves and TNs. The cell size of corneal TNs in the TG was calculated. All parameters were observed immediately (BAK group), at 1 week (1 w group), and 2 months (2 m group) after 2 weeks of BAK application. BAK caused an increase in the corneal staining score and the number of apoptotic cells, loss of conjunctival goblet cells, reduced density of corneal SNP, and upregulated expression of SP and inflammatory cytokines in both the cornea and TG in the BAK group but those changes were not observed in the 2 m group. On the other hand, SP-IR% and mean cell size of corneal TNs increased significantly in the BAK, 1 w, and 2 m groups, compared to the control. Our data suggest that following ocular surface inflammation, large-sized corneal TNs which normally do not express SP, expressed it and this phenotype switching lasted even after the inflammation disappeared. Long-lasting phenotypic switch, as well as changes in the expression level of certain molecules should be addressed in future studies on the mechanism of corneal neuropathic pain. Nature Publishing Group UK 2020-09-16 /pmc/articles/PMC7494893/ /pubmed/32939029 http://dx.doi.org/10.1038/s41598-020-72295-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Byun, Yong-Soo
Mok, Jee-Won
Chung, So-Hyang
Kim, Hyun-Seung
Joo, Choun-Ki
Ocular surface inflammation induces de novo expression of substance P in the trigeminal primary afferents with large cell bodies
title Ocular surface inflammation induces de novo expression of substance P in the trigeminal primary afferents with large cell bodies
title_full Ocular surface inflammation induces de novo expression of substance P in the trigeminal primary afferents with large cell bodies
title_fullStr Ocular surface inflammation induces de novo expression of substance P in the trigeminal primary afferents with large cell bodies
title_full_unstemmed Ocular surface inflammation induces de novo expression of substance P in the trigeminal primary afferents with large cell bodies
title_short Ocular surface inflammation induces de novo expression of substance P in the trigeminal primary afferents with large cell bodies
title_sort ocular surface inflammation induces de novo expression of substance p in the trigeminal primary afferents with large cell bodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494893/
https://www.ncbi.nlm.nih.gov/pubmed/32939029
http://dx.doi.org/10.1038/s41598-020-72295-x
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