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Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw
We here present the spontaneously immortalised cell line, HaSKpw, as a novel model for the multistep process of skin carcinogenesis. HaSKpw cells were established from the epidermis of normal human adult skin that, without crisis, are now growing unrestricted and feeder-independent. At passage 22, c...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494900/ https://www.ncbi.nlm.nih.gov/pubmed/32938951 http://dx.doi.org/10.1038/s41598-020-71315-0 |
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author | Pavez Lorie, Elizabeth Stricker, Nicola Plitta-Michalak, Beata Chen, I.-Peng Volkmer, Beate Greinert, Rüdiger Jauch, Anna Boukamp, Petra Rapp, Alexander |
author_facet | Pavez Lorie, Elizabeth Stricker, Nicola Plitta-Michalak, Beata Chen, I.-Peng Volkmer, Beate Greinert, Rüdiger Jauch, Anna Boukamp, Petra Rapp, Alexander |
author_sort | Pavez Lorie, Elizabeth |
collection | PubMed |
description | We here present the spontaneously immortalised cell line, HaSKpw, as a novel model for the multistep process of skin carcinogenesis. HaSKpw cells were established from the epidermis of normal human adult skin that, without crisis, are now growing unrestricted and feeder-independent. At passage 22, clonal populations were established and clone7 (HaSKpwC7) was further compared to the also spontaneously immortalized HaCaT cells. As important differences, the HaSKpw cells express wild-type p53, remain pseudodiploid, and show a unique chromosomal profile with numerous complex aberrations involving chromosome 20. In addition, HaSKpw cells overexpress a pattern of genes and miRNAs such as KRT34, LOX, S100A9, miR21, and miR155; all pointing to a tumorigenic status. In concordance, HaSKpw cells exhibit reduced desmosomal contacts that provide them with increased motility and a highly migratory/invasive phenotype as demonstrated in scratch- and Boyden chamber assays. In 3D organotypic cultures, both HaCaT and HaSKpw cells form disorganized epithelia but only the HaSKpw cells show tumorcell-like invasive growth. Together, HaSKpwC7 and HaCaT cells represent two spontaneous (non-genetically engineered) “premalignant” keratinocyte lines from adult human skin that display different stages of the multistep process of skin carcinogenesis and thus represent unique models for analysing skin cancer development and progression. |
format | Online Article Text |
id | pubmed-7494900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74949002020-09-18 Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw Pavez Lorie, Elizabeth Stricker, Nicola Plitta-Michalak, Beata Chen, I.-Peng Volkmer, Beate Greinert, Rüdiger Jauch, Anna Boukamp, Petra Rapp, Alexander Sci Rep Article We here present the spontaneously immortalised cell line, HaSKpw, as a novel model for the multistep process of skin carcinogenesis. HaSKpw cells were established from the epidermis of normal human adult skin that, without crisis, are now growing unrestricted and feeder-independent. At passage 22, clonal populations were established and clone7 (HaSKpwC7) was further compared to the also spontaneously immortalized HaCaT cells. As important differences, the HaSKpw cells express wild-type p53, remain pseudodiploid, and show a unique chromosomal profile with numerous complex aberrations involving chromosome 20. In addition, HaSKpw cells overexpress a pattern of genes and miRNAs such as KRT34, LOX, S100A9, miR21, and miR155; all pointing to a tumorigenic status. In concordance, HaSKpw cells exhibit reduced desmosomal contacts that provide them with increased motility and a highly migratory/invasive phenotype as demonstrated in scratch- and Boyden chamber assays. In 3D organotypic cultures, both HaCaT and HaSKpw cells form disorganized epithelia but only the HaSKpw cells show tumorcell-like invasive growth. Together, HaSKpwC7 and HaCaT cells represent two spontaneous (non-genetically engineered) “premalignant” keratinocyte lines from adult human skin that display different stages of the multistep process of skin carcinogenesis and thus represent unique models for analysing skin cancer development and progression. Nature Publishing Group UK 2020-09-16 /pmc/articles/PMC7494900/ /pubmed/32938951 http://dx.doi.org/10.1038/s41598-020-71315-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Pavez Lorie, Elizabeth Stricker, Nicola Plitta-Michalak, Beata Chen, I.-Peng Volkmer, Beate Greinert, Rüdiger Jauch, Anna Boukamp, Petra Rapp, Alexander Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw |
title | Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw |
title_full | Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw |
title_fullStr | Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw |
title_full_unstemmed | Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw |
title_short | Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw |
title_sort | characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line haskpw |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494900/ https://www.ncbi.nlm.nih.gov/pubmed/32938951 http://dx.doi.org/10.1038/s41598-020-71315-0 |
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