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Involvement of homodomain interacting protein kinase 2‐c‐Jun N‐terminal kinase/c‐Jun cascade in the long‐term synaptic toxicity and cognition impairment induced by neonatal Sevoflurane exposure
Sevoflurane is one of the most widely used anesthetics with recent concerns rising about its pediatric application. The synaptic toxicity and mechanisms underlying its long‐term cognition impairment remain unclear. In this study, we investigated the expression and roles of homeodomain interacting pr...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496229/ https://www.ncbi.nlm.nih.gov/pubmed/31705656 http://dx.doi.org/10.1111/jnc.14910 |
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author | Liang, Lirong Xie, Rougang Lu, Rui Ma, Ruixue Wang, Xiaoxia Wang, Fengjuan Liu, Bing Wu, Shengxi Wang, Yazhou Zhang, Hui |
author_facet | Liang, Lirong Xie, Rougang Lu, Rui Ma, Ruixue Wang, Xiaoxia Wang, Fengjuan Liu, Bing Wu, Shengxi Wang, Yazhou Zhang, Hui |
author_sort | Liang, Lirong |
collection | PubMed |
description | Sevoflurane is one of the most widely used anesthetics with recent concerns rising about its pediatric application. The synaptic toxicity and mechanisms underlying its long‐term cognition impairment remain unclear. In this study, we investigated the expression and roles of homeodomain interacting protein kinase 2 (HIPK2), a stress activating kinase involved in neuronal survival and synaptic plasticity, and its downstream c‐Jun N‐terminal kinase (JNK)/c‐Jun signaling in the long‐term toxicity of neonatal Sevoflurane exposure. Our data showed that neonatal Sevoflurane exposure results in impairment of memory, enhancement of anxiety, less number of excitatory synapses and lower levels of synaptic proteins in the hippocampus of adult rats without significant changes of hippocampal neuron numbers. Up‐regulation of HIPK2 and JNK/c‐Jun was observed in hippocampal granular neurons shortly after Sevoflurane exposure and persisted to adult. 5‐((6‐Oxo‐5‐(6‐(piperazin‐1‐yl)pyridin‐3‐yl)‐1,6‐dihydropyridin‐3‐yl)methylene)thiazolidine‐2,4‐dione trifluoroacetate, antagonist of HIPK2, could significantly rescue the cognition impairment, decrease in long‐term potentiation, reduction in spine density and activation of JNK/c‐Jun induced by Sevoflurane. JNK antagonist SP600125 partially restored synapse development and cognitive function without affecting the expression of HIPK2. These data, in together, revealed a novel role of HIPK2‐JNK/c‐Jun signaling in the long‐term synaptic toxicity and cognition impairment of neonatal Sevoflurane exposure, indicating HIPK2‐JNK/c‐Jun cascade as a potential target for reducing the synaptic toxicity of Sevoflurane. [Image: see text] Cover Image for this issue: doi: 10.1111/jnc.14757. |
format | Online Article Text |
id | pubmed-7496229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74962292020-09-25 Involvement of homodomain interacting protein kinase 2‐c‐Jun N‐terminal kinase/c‐Jun cascade in the long‐term synaptic toxicity and cognition impairment induced by neonatal Sevoflurane exposure Liang, Lirong Xie, Rougang Lu, Rui Ma, Ruixue Wang, Xiaoxia Wang, Fengjuan Liu, Bing Wu, Shengxi Wang, Yazhou Zhang, Hui J Neurochem ORIGINAL ARTICLES Sevoflurane is one of the most widely used anesthetics with recent concerns rising about its pediatric application. The synaptic toxicity and mechanisms underlying its long‐term cognition impairment remain unclear. In this study, we investigated the expression and roles of homeodomain interacting protein kinase 2 (HIPK2), a stress activating kinase involved in neuronal survival and synaptic plasticity, and its downstream c‐Jun N‐terminal kinase (JNK)/c‐Jun signaling in the long‐term toxicity of neonatal Sevoflurane exposure. Our data showed that neonatal Sevoflurane exposure results in impairment of memory, enhancement of anxiety, less number of excitatory synapses and lower levels of synaptic proteins in the hippocampus of adult rats without significant changes of hippocampal neuron numbers. Up‐regulation of HIPK2 and JNK/c‐Jun was observed in hippocampal granular neurons shortly after Sevoflurane exposure and persisted to adult. 5‐((6‐Oxo‐5‐(6‐(piperazin‐1‐yl)pyridin‐3‐yl)‐1,6‐dihydropyridin‐3‐yl)methylene)thiazolidine‐2,4‐dione trifluoroacetate, antagonist of HIPK2, could significantly rescue the cognition impairment, decrease in long‐term potentiation, reduction in spine density and activation of JNK/c‐Jun induced by Sevoflurane. JNK antagonist SP600125 partially restored synapse development and cognitive function without affecting the expression of HIPK2. These data, in together, revealed a novel role of HIPK2‐JNK/c‐Jun signaling in the long‐term synaptic toxicity and cognition impairment of neonatal Sevoflurane exposure, indicating HIPK2‐JNK/c‐Jun cascade as a potential target for reducing the synaptic toxicity of Sevoflurane. [Image: see text] Cover Image for this issue: doi: 10.1111/jnc.14757. John Wiley and Sons Inc. 2020-01-19 2020-08 /pmc/articles/PMC7496229/ /pubmed/31705656 http://dx.doi.org/10.1111/jnc.14910 Text en © 2019 The Authors. Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | ORIGINAL ARTICLES Liang, Lirong Xie, Rougang Lu, Rui Ma, Ruixue Wang, Xiaoxia Wang, Fengjuan Liu, Bing Wu, Shengxi Wang, Yazhou Zhang, Hui Involvement of homodomain interacting protein kinase 2‐c‐Jun N‐terminal kinase/c‐Jun cascade in the long‐term synaptic toxicity and cognition impairment induced by neonatal Sevoflurane exposure |
title | Involvement of homodomain interacting protein kinase 2‐c‐Jun N‐terminal kinase/c‐Jun cascade in the long‐term synaptic toxicity and cognition impairment induced by neonatal Sevoflurane exposure |
title_full | Involvement of homodomain interacting protein kinase 2‐c‐Jun N‐terminal kinase/c‐Jun cascade in the long‐term synaptic toxicity and cognition impairment induced by neonatal Sevoflurane exposure |
title_fullStr | Involvement of homodomain interacting protein kinase 2‐c‐Jun N‐terminal kinase/c‐Jun cascade in the long‐term synaptic toxicity and cognition impairment induced by neonatal Sevoflurane exposure |
title_full_unstemmed | Involvement of homodomain interacting protein kinase 2‐c‐Jun N‐terminal kinase/c‐Jun cascade in the long‐term synaptic toxicity and cognition impairment induced by neonatal Sevoflurane exposure |
title_short | Involvement of homodomain interacting protein kinase 2‐c‐Jun N‐terminal kinase/c‐Jun cascade in the long‐term synaptic toxicity and cognition impairment induced by neonatal Sevoflurane exposure |
title_sort | involvement of homodomain interacting protein kinase 2‐c‐jun n‐terminal kinase/c‐jun cascade in the long‐term synaptic toxicity and cognition impairment induced by neonatal sevoflurane exposure |
topic | ORIGINAL ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496229/ https://www.ncbi.nlm.nih.gov/pubmed/31705656 http://dx.doi.org/10.1111/jnc.14910 |
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