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The Arabidopsis mTERF‐repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons

The mTERF gene family encodes for nucleic acid binding proteins that are predicted to regulate organellar gene expression in eukaryotes. Despite the implication of this gene family in plant development and response to abiotic stresses, a precise molecular function was assigned to only a handful numb...

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Autores principales: Méteignier, Louis‐Valentin, Ghandour, Rabea, Meierhoff, Karin, Zimmerman, Aude, Chicher, Johana, Baumberger, Nicolas, Alioua, Abdelmalek, Meurer, Jörg, Zoschke, Reimo, Hammani, Kamel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496394/
https://www.ncbi.nlm.nih.gov/pubmed/32343843
http://dx.doi.org/10.1111/nph.16625
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author Méteignier, Louis‐Valentin
Ghandour, Rabea
Meierhoff, Karin
Zimmerman, Aude
Chicher, Johana
Baumberger, Nicolas
Alioua, Abdelmalek
Meurer, Jörg
Zoschke, Reimo
Hammani, Kamel
author_facet Méteignier, Louis‐Valentin
Ghandour, Rabea
Meierhoff, Karin
Zimmerman, Aude
Chicher, Johana
Baumberger, Nicolas
Alioua, Abdelmalek
Meurer, Jörg
Zoschke, Reimo
Hammani, Kamel
author_sort Méteignier, Louis‐Valentin
collection PubMed
description The mTERF gene family encodes for nucleic acid binding proteins that are predicted to regulate organellar gene expression in eukaryotes. Despite the implication of this gene family in plant development and response to abiotic stresses, a precise molecular function was assigned to only a handful number of its c. 30 members in plants. Using a reverse genetics approach in Arabidopsis thaliana and combining molecular and biochemical techniques, we revealed new functions for the chloroplast mTERF protein, MDA1. We demonstrated that MDA1 associates in vivo with components of the plastid‐encoded RNA polymerase and transcriptional active chromosome complexes. MDA1 protein binds in vivo and in vitro with specificity to 27‐bp DNA sequences near the 5′‐end of psbE and ndhA chloroplast genes to stimulate their transcription, and additionally promotes the stabilization of the 5′‐ends of processed psbE and ndhA messenger (m)RNAs. Finally, we provided evidence that MDA1 function in gene transcription likely coordinates RNA folding and the action of chloroplast RNA‐binding proteins on mRNA stabilization. Our results provide examples for the unexpected implication of DNA binding proteins and gene transcription in the regulation of mRNA stability in chloroplasts, blurring the boundaries between DNA and RNA metabolism in this organelle.
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spelling pubmed-74963942020-09-25 The Arabidopsis mTERF‐repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons Méteignier, Louis‐Valentin Ghandour, Rabea Meierhoff, Karin Zimmerman, Aude Chicher, Johana Baumberger, Nicolas Alioua, Abdelmalek Meurer, Jörg Zoschke, Reimo Hammani, Kamel New Phytol Research The mTERF gene family encodes for nucleic acid binding proteins that are predicted to regulate organellar gene expression in eukaryotes. Despite the implication of this gene family in plant development and response to abiotic stresses, a precise molecular function was assigned to only a handful number of its c. 30 members in plants. Using a reverse genetics approach in Arabidopsis thaliana and combining molecular and biochemical techniques, we revealed new functions for the chloroplast mTERF protein, MDA1. We demonstrated that MDA1 associates in vivo with components of the plastid‐encoded RNA polymerase and transcriptional active chromosome complexes. MDA1 protein binds in vivo and in vitro with specificity to 27‐bp DNA sequences near the 5′‐end of psbE and ndhA chloroplast genes to stimulate their transcription, and additionally promotes the stabilization of the 5′‐ends of processed psbE and ndhA messenger (m)RNAs. Finally, we provided evidence that MDA1 function in gene transcription likely coordinates RNA folding and the action of chloroplast RNA‐binding proteins on mRNA stabilization. Our results provide examples for the unexpected implication of DNA binding proteins and gene transcription in the regulation of mRNA stability in chloroplasts, blurring the boundaries between DNA and RNA metabolism in this organelle. John Wiley and Sons Inc. 2020-05-26 2020-09 /pmc/articles/PMC7496394/ /pubmed/32343843 http://dx.doi.org/10.1111/nph.16625 Text en © 2020 The Authors. New Phytologist © 2020 New Phytologist Trust This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research
Méteignier, Louis‐Valentin
Ghandour, Rabea
Meierhoff, Karin
Zimmerman, Aude
Chicher, Johana
Baumberger, Nicolas
Alioua, Abdelmalek
Meurer, Jörg
Zoschke, Reimo
Hammani, Kamel
The Arabidopsis mTERF‐repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons
title The Arabidopsis mTERF‐repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons
title_full The Arabidopsis mTERF‐repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons
title_fullStr The Arabidopsis mTERF‐repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons
title_full_unstemmed The Arabidopsis mTERF‐repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons
title_short The Arabidopsis mTERF‐repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons
title_sort arabidopsis mterf‐repeat mda1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbe and ndhh operons
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496394/
https://www.ncbi.nlm.nih.gov/pubmed/32343843
http://dx.doi.org/10.1111/nph.16625
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