Cargando…
Resident mesenchymal vascular progenitors modulate adaptive angiogenesis and pulmonary remodeling via regulation of canonical Wnt signaling
Adaptive angiogenesis is necessary for tissue repair, however, it may also be associated with the exacerbation of injury and development of chronic disease. In these studies, we demonstrate that lung mesenchymal vascular progenitor cells (MVPC) modulate adaptive angiogenesis via lineage trace, deple...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496763/ https://www.ncbi.nlm.nih.gov/pubmed/32533805 http://dx.doi.org/10.1096/fj.202000629R |
_version_ | 1783583170371780608 |
---|---|
author | Summers, Megan E. Richmond, Bradley W. Menon, Swapna Sheridan, Ryan M. Kropski, Jonathan A. Majka, Sarah A. Taketo, M. Mark Bastarache, Julie A. West, James D. De Langhe, Stijn Geraghty, Patrick Klemm, Dwight J. Chu, Hong Wei Friedman, Rachel S. Tao, Yuankai K. Foronjy, Robert F. Majka, Susan M. |
author_facet | Summers, Megan E. Richmond, Bradley W. Menon, Swapna Sheridan, Ryan M. Kropski, Jonathan A. Majka, Sarah A. Taketo, M. Mark Bastarache, Julie A. West, James D. De Langhe, Stijn Geraghty, Patrick Klemm, Dwight J. Chu, Hong Wei Friedman, Rachel S. Tao, Yuankai K. Foronjy, Robert F. Majka, Susan M. |
author_sort | Summers, Megan E. |
collection | PubMed |
description | Adaptive angiogenesis is necessary for tissue repair, however, it may also be associated with the exacerbation of injury and development of chronic disease. In these studies, we demonstrate that lung mesenchymal vascular progenitor cells (MVPC) modulate adaptive angiogenesis via lineage trace, depletion of MVPC, and modulation of β‐catenin expression. Single cell sequencing confirmed MVPC as multipotential vascular progenitors, thus, genetic depletion resulted in alveolar simplification with reduced adaptive angiogenesis. Following vascular endothelial injury, Wnt activation in MVPC was sufficient to elicit an emphysema‐like phenotype characterized by increased MLI, fibrosis, and MVPC driven adaptive angiogenesis. Lastly, activation of Wnt/β‐catenin signaling skewed the profile of human and murine MVPC toward an adaptive phenotype. These data suggest that lung MVPC drive angiogenesis in response to injury and regulate the microvascular niche as well as subsequent distal lung tissue architecture via Wnt signaling. |
format | Online Article Text |
id | pubmed-7496763 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74967632020-09-25 Resident mesenchymal vascular progenitors modulate adaptive angiogenesis and pulmonary remodeling via regulation of canonical Wnt signaling Summers, Megan E. Richmond, Bradley W. Menon, Swapna Sheridan, Ryan M. Kropski, Jonathan A. Majka, Sarah A. Taketo, M. Mark Bastarache, Julie A. West, James D. De Langhe, Stijn Geraghty, Patrick Klemm, Dwight J. Chu, Hong Wei Friedman, Rachel S. Tao, Yuankai K. Foronjy, Robert F. Majka, Susan M. FASEB J Research Articles Adaptive angiogenesis is necessary for tissue repair, however, it may also be associated with the exacerbation of injury and development of chronic disease. In these studies, we demonstrate that lung mesenchymal vascular progenitor cells (MVPC) modulate adaptive angiogenesis via lineage trace, depletion of MVPC, and modulation of β‐catenin expression. Single cell sequencing confirmed MVPC as multipotential vascular progenitors, thus, genetic depletion resulted in alveolar simplification with reduced adaptive angiogenesis. Following vascular endothelial injury, Wnt activation in MVPC was sufficient to elicit an emphysema‐like phenotype characterized by increased MLI, fibrosis, and MVPC driven adaptive angiogenesis. Lastly, activation of Wnt/β‐catenin signaling skewed the profile of human and murine MVPC toward an adaptive phenotype. These data suggest that lung MVPC drive angiogenesis in response to injury and regulate the microvascular niche as well as subsequent distal lung tissue architecture via Wnt signaling. John Wiley and Sons Inc. 2020-06-13 2020-08 /pmc/articles/PMC7496763/ /pubmed/32533805 http://dx.doi.org/10.1096/fj.202000629R Text en © 2020 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Summers, Megan E. Richmond, Bradley W. Menon, Swapna Sheridan, Ryan M. Kropski, Jonathan A. Majka, Sarah A. Taketo, M. Mark Bastarache, Julie A. West, James D. De Langhe, Stijn Geraghty, Patrick Klemm, Dwight J. Chu, Hong Wei Friedman, Rachel S. Tao, Yuankai K. Foronjy, Robert F. Majka, Susan M. Resident mesenchymal vascular progenitors modulate adaptive angiogenesis and pulmonary remodeling via regulation of canonical Wnt signaling |
title | Resident mesenchymal vascular progenitors modulate adaptive angiogenesis and pulmonary remodeling via regulation of canonical Wnt signaling |
title_full | Resident mesenchymal vascular progenitors modulate adaptive angiogenesis and pulmonary remodeling via regulation of canonical Wnt signaling |
title_fullStr | Resident mesenchymal vascular progenitors modulate adaptive angiogenesis and pulmonary remodeling via regulation of canonical Wnt signaling |
title_full_unstemmed | Resident mesenchymal vascular progenitors modulate adaptive angiogenesis and pulmonary remodeling via regulation of canonical Wnt signaling |
title_short | Resident mesenchymal vascular progenitors modulate adaptive angiogenesis and pulmonary remodeling via regulation of canonical Wnt signaling |
title_sort | resident mesenchymal vascular progenitors modulate adaptive angiogenesis and pulmonary remodeling via regulation of canonical wnt signaling |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496763/ https://www.ncbi.nlm.nih.gov/pubmed/32533805 http://dx.doi.org/10.1096/fj.202000629R |
work_keys_str_mv | AT summersmegane residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT richmondbradleyw residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT menonswapna residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT sheridanryanm residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT kropskijonathana residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT majkasaraha residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT taketommark residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT bastarachejuliea residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT westjamesd residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT delanghestijn residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT geraghtypatrick residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT klemmdwightj residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT chuhongwei residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT friedmanrachels residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT taoyuankaik residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT foronjyrobertf residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling AT majkasusanm residentmesenchymalvascularprogenitorsmodulateadaptiveangiogenesisandpulmonaryremodelingviaregulationofcanonicalwntsignaling |