Quantitative Formation of Monomeric G‐Quadruplex DNA from Multimeric Structures of c‐Myc Promoter Sequence

G‐Quadruplex (G4)‐forming DNA sequences have a tendency to form stable multimeric structures. This can be problematic for studies with synthetic oligodeoxynucleotides. Herein, we describe a method that quantitatively converts multimeric intermolecular structures of the Pu27 sequence from the c‐myc p...

Descripción completa

Detalles Bibliográficos
Autores principales: Rauser, Valerie, Weinhold, Elmar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496815/
https://www.ncbi.nlm.nih.gov/pubmed/32267052
http://dx.doi.org/10.1002/cbic.202000159
Descripción
Sumario:G‐Quadruplex (G4)‐forming DNA sequences have a tendency to form stable multimeric structures. This can be problematic for studies with synthetic oligodeoxynucleotides. Herein, we describe a method that quantitatively converts multimeric intermolecular structures of the Pu27 sequence from the c‐myc promoter into the desired monomeric G4 by alkaline treatment and refolding.

Ejemplares similares