Quantitative Formation of Monomeric G‐Quadruplex DNA from Multimeric Structures of c‐Myc Promoter Sequence

G‐Quadruplex (G4)‐forming DNA sequences have a tendency to form stable multimeric structures. This can be problematic for studies with synthetic oligodeoxynucleotides. Herein, we describe a method that quantitatively converts multimeric intermolecular structures of the Pu27 sequence from the c‐myc p...

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Detalles Bibliográficos
Autores principales: Rauser, Valerie, Weinhold, Elmar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496815/
https://www.ncbi.nlm.nih.gov/pubmed/32267052
http://dx.doi.org/10.1002/cbic.202000159
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author Rauser, Valerie
Weinhold, Elmar
author_facet Rauser, Valerie
Weinhold, Elmar
author_sort Rauser, Valerie
collection PubMed
description G‐Quadruplex (G4)‐forming DNA sequences have a tendency to form stable multimeric structures. This can be problematic for studies with synthetic oligodeoxynucleotides. Herein, we describe a method that quantitatively converts multimeric intermolecular structures of the Pu27 sequence from the c‐myc promoter into the desired monomeric G4 by alkaline treatment and refolding.
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spelling pubmed-74968152020-09-25 Quantitative Formation of Monomeric G‐Quadruplex DNA from Multimeric Structures of c‐Myc Promoter Sequence Rauser, Valerie Weinhold, Elmar Chembiochem Communications G‐Quadruplex (G4)‐forming DNA sequences have a tendency to form stable multimeric structures. This can be problematic for studies with synthetic oligodeoxynucleotides. Herein, we describe a method that quantitatively converts multimeric intermolecular structures of the Pu27 sequence from the c‐myc promoter into the desired monomeric G4 by alkaline treatment and refolding. John Wiley and Sons Inc. 2020-05-11 2020-09-01 /pmc/articles/PMC7496815/ /pubmed/32267052 http://dx.doi.org/10.1002/cbic.202000159 Text en © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Rauser, Valerie
Weinhold, Elmar
Quantitative Formation of Monomeric G‐Quadruplex DNA from Multimeric Structures of c‐Myc Promoter Sequence
title Quantitative Formation of Monomeric G‐Quadruplex DNA from Multimeric Structures of c‐Myc Promoter Sequence
title_full Quantitative Formation of Monomeric G‐Quadruplex DNA from Multimeric Structures of c‐Myc Promoter Sequence
title_fullStr Quantitative Formation of Monomeric G‐Quadruplex DNA from Multimeric Structures of c‐Myc Promoter Sequence
title_full_unstemmed Quantitative Formation of Monomeric G‐Quadruplex DNA from Multimeric Structures of c‐Myc Promoter Sequence
title_short Quantitative Formation of Monomeric G‐Quadruplex DNA from Multimeric Structures of c‐Myc Promoter Sequence
title_sort quantitative formation of monomeric g‐quadruplex dna from multimeric structures of c‐myc promoter sequence
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496815/
https://www.ncbi.nlm.nih.gov/pubmed/32267052
http://dx.doi.org/10.1002/cbic.202000159
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