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Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction

The display of complex proteins on the surface of cells is of great importance for protein engineering and other fields of biotechnology. Herein, we describe a modular approach, in which the membrane anchor protein Lpp‐OmpA and a protein of interest (passenger) are expressed independently as genetic...

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Detalles Bibliográficos
Autores principales: Gallus, Sabrina, Peschke, Theo, Paulsen, Malte, Burgahn, Teresa, Niemeyer, Christof M., Rabe, Kersten S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497234/
https://www.ncbi.nlm.nih.gov/pubmed/32182402
http://dx.doi.org/10.1002/cbic.202000102