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Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction
The display of complex proteins on the surface of cells is of great importance for protein engineering and other fields of biotechnology. Herein, we describe a modular approach, in which the membrane anchor protein Lpp‐OmpA and a protein of interest (passenger) are expressed independently as genetic...
Autores principales: | Gallus, Sabrina, Peschke, Theo, Paulsen, Malte, Burgahn, Teresa, Niemeyer, Christof M., Rabe, Kersten S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497234/ https://www.ncbi.nlm.nih.gov/pubmed/32182402 http://dx.doi.org/10.1002/cbic.202000102 |
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