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Identification and characterization of PEDV infection in rat crypt epithelial cells
Porcine epidemic diarrhea (PED) is a devastating enteric disease to the world's swine production. Porcine epidemic diarrhea virus (PEDV), as the PED causative agent, has been commonly propagated and investigated in Vero cells, as well as in IPEC-J2, a porcine epithelial cell-jejunum 2. However,...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497550/ https://www.ncbi.nlm.nih.gov/pubmed/32979749 http://dx.doi.org/10.1016/j.vetmic.2020.108848 |
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author | Chen, Jianing Cui, Yaru Wang, Zemei Liu, Guangliang |
author_facet | Chen, Jianing Cui, Yaru Wang, Zemei Liu, Guangliang |
author_sort | Chen, Jianing |
collection | PubMed |
description | Porcine epidemic diarrhea (PED) is a devastating enteric disease to the world's swine production. Porcine epidemic diarrhea virus (PEDV), as the PED causative agent, has been commonly propagated and investigated in Vero cells, as well as in IPEC-J2, a porcine epithelial cell-jejunum 2. However, Vero cells, which are defective in interferon production, cannot represent the host response in enteric cells while PEDV replicates poorly in IPEC-J2 cells. In this study, we observed that rat crypt epithelial cells (IEC-6) were highly susceptible to different subtypes of PEDV. The replication kinetics of PEDV in IEC-6 cells is similar to that in Vero cells, but it is much higher than in IPEC-J2 cells. Besides that, PEDV infection in IEC-6 cells can induce the production of inflammatory cytokines and interferon, especially the type III IFNs. Collectively, our findings suggest that IEC-6 is an ideal cell line for PEDV replication and immune response studies. |
format | Online Article Text |
id | pubmed-7497550 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74975502020-09-18 Identification and characterization of PEDV infection in rat crypt epithelial cells Chen, Jianing Cui, Yaru Wang, Zemei Liu, Guangliang Vet Microbiol Short Communication Porcine epidemic diarrhea (PED) is a devastating enteric disease to the world's swine production. Porcine epidemic diarrhea virus (PEDV), as the PED causative agent, has been commonly propagated and investigated in Vero cells, as well as in IPEC-J2, a porcine epithelial cell-jejunum 2. However, Vero cells, which are defective in interferon production, cannot represent the host response in enteric cells while PEDV replicates poorly in IPEC-J2 cells. In this study, we observed that rat crypt epithelial cells (IEC-6) were highly susceptible to different subtypes of PEDV. The replication kinetics of PEDV in IEC-6 cells is similar to that in Vero cells, but it is much higher than in IPEC-J2 cells. Besides that, PEDV infection in IEC-6 cells can induce the production of inflammatory cytokines and interferon, especially the type III IFNs. Collectively, our findings suggest that IEC-6 is an ideal cell line for PEDV replication and immune response studies. Elsevier B.V. 2020-10 2020-09-17 /pmc/articles/PMC7497550/ /pubmed/32979749 http://dx.doi.org/10.1016/j.vetmic.2020.108848 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Short Communication Chen, Jianing Cui, Yaru Wang, Zemei Liu, Guangliang Identification and characterization of PEDV infection in rat crypt epithelial cells |
title | Identification and characterization of PEDV infection in rat crypt epithelial cells |
title_full | Identification and characterization of PEDV infection in rat crypt epithelial cells |
title_fullStr | Identification and characterization of PEDV infection in rat crypt epithelial cells |
title_full_unstemmed | Identification and characterization of PEDV infection in rat crypt epithelial cells |
title_short | Identification and characterization of PEDV infection in rat crypt epithelial cells |
title_sort | identification and characterization of pedv infection in rat crypt epithelial cells |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497550/ https://www.ncbi.nlm.nih.gov/pubmed/32979749 http://dx.doi.org/10.1016/j.vetmic.2020.108848 |
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