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Colistin Resistance of Non-pathogenic Strains of Escherichia Coli Occurring as Natural Intestinal Flora in Broiler Chickens Treated and not Treated with Colistin Sulphate
INTRODUCTION: A significant threat to public health is presented by antibiotic-resistant strains of bacteria, selective pressure on which results from antibiotic use. Colistin is an antibiotic commonly used in veterinary medicine, but also one of last resort in human medicine. Since the 2015 discove...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sciendo
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497749/ https://www.ncbi.nlm.nih.gov/pubmed/32984630 http://dx.doi.org/10.2478/jvetres-2020-0060 |
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author | Majewski, Michał Łukomska, Anna Wilczyński, Jarosław Wystalska, Danuta Racewicz, Przemysław Nowacka-Woszuk, Joanna Pszczola, Marcin Anusz, Krzysztof |
author_facet | Majewski, Michał Łukomska, Anna Wilczyński, Jarosław Wystalska, Danuta Racewicz, Przemysław Nowacka-Woszuk, Joanna Pszczola, Marcin Anusz, Krzysztof |
author_sort | Majewski, Michał |
collection | PubMed |
description | INTRODUCTION: A significant threat to public health is presented by antibiotic-resistant strains of bacteria, selective pressure on which results from antibiotic use. Colistin is an antibiotic commonly used in veterinary medicine, but also one of last resort in human medicine. Since the 2015 discovery in China of the mcr-1 gene encoding colistin resistance in Enterobacteriaceae, other countries have noted its presence. This study was to find the mcr-1 gene prevalence in E. coli isolated from poultry slaughtered in Poland. MATERIAL AND METHODS: Cloacal swabs were taken from December 2017 to October 2018 from broiler chickens in three regions. The samples (n = 158) were grouped as flocks treated with colistin sulphate (n = 87) and those not treated (n = 71). Resistance to antimicrobials commonly used in poultry was evaluated by minimum inhibitory concentration. The presence of the mcr-1 gene was confirmed by PCR. RESULTS: Isolates containing the mcr-1 gene were yielded by 11.27% of the samples from not treated flocks and 19.54% of those from treated flocks, but no statistically significant difference in the prevalence of the gene was seen between the groups. CONCLUSION: The results clearly preclude intensification of selective pressure for colistin resistance due to colistin sulphate treatment because they show that the avian gastrointestinal tract was already inhabited by colistin-resistant E. coli by the time the chickens came to the poultry house. |
format | Online Article Text |
id | pubmed-7497749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-74977492020-09-25 Colistin Resistance of Non-pathogenic Strains of Escherichia Coli Occurring as Natural Intestinal Flora in Broiler Chickens Treated and not Treated with Colistin Sulphate Majewski, Michał Łukomska, Anna Wilczyński, Jarosław Wystalska, Danuta Racewicz, Przemysław Nowacka-Woszuk, Joanna Pszczola, Marcin Anusz, Krzysztof J Vet Res Research Article INTRODUCTION: A significant threat to public health is presented by antibiotic-resistant strains of bacteria, selective pressure on which results from antibiotic use. Colistin is an antibiotic commonly used in veterinary medicine, but also one of last resort in human medicine. Since the 2015 discovery in China of the mcr-1 gene encoding colistin resistance in Enterobacteriaceae, other countries have noted its presence. This study was to find the mcr-1 gene prevalence in E. coli isolated from poultry slaughtered in Poland. MATERIAL AND METHODS: Cloacal swabs were taken from December 2017 to October 2018 from broiler chickens in three regions. The samples (n = 158) were grouped as flocks treated with colistin sulphate (n = 87) and those not treated (n = 71). Resistance to antimicrobials commonly used in poultry was evaluated by minimum inhibitory concentration. The presence of the mcr-1 gene was confirmed by PCR. RESULTS: Isolates containing the mcr-1 gene were yielded by 11.27% of the samples from not treated flocks and 19.54% of those from treated flocks, but no statistically significant difference in the prevalence of the gene was seen between the groups. CONCLUSION: The results clearly preclude intensification of selective pressure for colistin resistance due to colistin sulphate treatment because they show that the avian gastrointestinal tract was already inhabited by colistin-resistant E. coli by the time the chickens came to the poultry house. Sciendo 2020-09-16 /pmc/articles/PMC7497749/ /pubmed/32984630 http://dx.doi.org/10.2478/jvetres-2020-0060 Text en © 2020 M. Majewski et al. published by Sciendo http://creativecommons.org/licenses/by-nc-nd/3.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. |
spellingShingle | Research Article Majewski, Michał Łukomska, Anna Wilczyński, Jarosław Wystalska, Danuta Racewicz, Przemysław Nowacka-Woszuk, Joanna Pszczola, Marcin Anusz, Krzysztof Colistin Resistance of Non-pathogenic Strains of Escherichia Coli Occurring as Natural Intestinal Flora in Broiler Chickens Treated and not Treated with Colistin Sulphate |
title | Colistin Resistance of Non-pathogenic Strains of Escherichia Coli Occurring as Natural Intestinal Flora in Broiler Chickens Treated and not Treated with Colistin Sulphate |
title_full | Colistin Resistance of Non-pathogenic Strains of Escherichia Coli Occurring as Natural Intestinal Flora in Broiler Chickens Treated and not Treated with Colistin Sulphate |
title_fullStr | Colistin Resistance of Non-pathogenic Strains of Escherichia Coli Occurring as Natural Intestinal Flora in Broiler Chickens Treated and not Treated with Colistin Sulphate |
title_full_unstemmed | Colistin Resistance of Non-pathogenic Strains of Escherichia Coli Occurring as Natural Intestinal Flora in Broiler Chickens Treated and not Treated with Colistin Sulphate |
title_short | Colistin Resistance of Non-pathogenic Strains of Escherichia Coli Occurring as Natural Intestinal Flora in Broiler Chickens Treated and not Treated with Colistin Sulphate |
title_sort | colistin resistance of non-pathogenic strains of escherichia coli occurring as natural intestinal flora in broiler chickens treated and not treated with colistin sulphate |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497749/ https://www.ncbi.nlm.nih.gov/pubmed/32984630 http://dx.doi.org/10.2478/jvetres-2020-0060 |
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