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Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012–2018
OBJECTIVE. To describe the resistance profile and the genetic characteristics of Escherichia coli isolates that harbor the mobilizable colistin resistance gene mcr-1 in Argentina. METHODS. This was a retrospective study of 192 E. coli isolates positive for mcr-1 obtained from 69 hospitals of Buenos...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Organización Panamericana de la Salud
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498280/ https://www.ncbi.nlm.nih.gov/pubmed/32973904 http://dx.doi.org/10.26633/RPSP.2020.55 |
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author | Faccone, Diego Rapoport, Melina Albornoz, Ezequiel Celaya, Federico De Mendieta, Juan De Belder, Denise Lucero, Celeste Gomez, Sonia Danze, Diego Pasteran, Fernando Corso, Alejandra |
author_facet | Faccone, Diego Rapoport, Melina Albornoz, Ezequiel Celaya, Federico De Mendieta, Juan De Belder, Denise Lucero, Celeste Gomez, Sonia Danze, Diego Pasteran, Fernando Corso, Alejandra |
author_sort | Faccone, Diego |
collection | PubMed |
description | OBJECTIVE. To describe the resistance profile and the genetic characteristics of Escherichia coli isolates that harbor the mobilizable colistin resistance gene mcr-1 in Argentina. METHODS. This was a retrospective study of 192 E. coli isolates positive for mcr-1 obtained from 69 hospitals of Buenos Aires City and 14 Argentinean provinces in 2012 – 2018. The antimicrobial susceptibility was performed by agar diffusion, broth macrodilution, and/or agar dilution. Standard polymerase chain reaction (PCR) was performed to detect resistance genes and incompatibility groups; specific PCR was applied to discriminate between blaCTX-M allelic groups and mcr-1.5 variant. The genetic relatedness among isolates was evaluated by XbaI-pulsed field gel electrophoresis and multilocus sequence typing in a subset of isolates. RESULTS. All E. coli isolates showed minimal inhibitory concentrations to colistin ≥ 4μg/mL; nearly 50% were resistant to third-generation cephalosporins, with CTX-M-2 being the main extended-spectrum β-lactamase detected. Five E. coli were carbapenemase-producers (3 NDM, 2 KPC). The mcr-1.5 variant was detected in 13.5% of the isolates. No genetic relationship was observed among the mcr-1-positive E. coli clinical isolates, but a high proportion (164/192; 85.4%) of IncI2 plasmids was detected. CONCLUSIONS. The presence of IncI2 plasmids among highly diverse E. coli clones suggests that the mcr-1 gene’s wide distribution in Argentina may be driven by the horizontal transmission of IncI2 plasmids. |
format | Online Article Text |
id | pubmed-7498280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Organización Panamericana de la Salud |
record_format | MEDLINE/PubMed |
spelling | pubmed-74982802020-09-23 Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012–2018 Faccone, Diego Rapoport, Melina Albornoz, Ezequiel Celaya, Federico De Mendieta, Juan De Belder, Denise Lucero, Celeste Gomez, Sonia Danze, Diego Pasteran, Fernando Corso, Alejandra Rev Panam Salud Publica Original Research OBJECTIVE. To describe the resistance profile and the genetic characteristics of Escherichia coli isolates that harbor the mobilizable colistin resistance gene mcr-1 in Argentina. METHODS. This was a retrospective study of 192 E. coli isolates positive for mcr-1 obtained from 69 hospitals of Buenos Aires City and 14 Argentinean provinces in 2012 – 2018. The antimicrobial susceptibility was performed by agar diffusion, broth macrodilution, and/or agar dilution. Standard polymerase chain reaction (PCR) was performed to detect resistance genes and incompatibility groups; specific PCR was applied to discriminate between blaCTX-M allelic groups and mcr-1.5 variant. The genetic relatedness among isolates was evaluated by XbaI-pulsed field gel electrophoresis and multilocus sequence typing in a subset of isolates. RESULTS. All E. coli isolates showed minimal inhibitory concentrations to colistin ≥ 4μg/mL; nearly 50% were resistant to third-generation cephalosporins, with CTX-M-2 being the main extended-spectrum β-lactamase detected. Five E. coli were carbapenemase-producers (3 NDM, 2 KPC). The mcr-1.5 variant was detected in 13.5% of the isolates. No genetic relationship was observed among the mcr-1-positive E. coli clinical isolates, but a high proportion (164/192; 85.4%) of IncI2 plasmids was detected. CONCLUSIONS. The presence of IncI2 plasmids among highly diverse E. coli clones suggests that the mcr-1 gene’s wide distribution in Argentina may be driven by the horizontal transmission of IncI2 plasmids. Organización Panamericana de la Salud 2020-09-23 /pmc/articles/PMC7498280/ /pubmed/32973904 http://dx.doi.org/10.26633/RPSP.2020.55 Text en https://creativecommons.org/licenses/by-nc-nd/3.0/igo/legalcode This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 IGO License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited. No modifications or commercial use of this article are permitted. In any reproduction of this article there should not be any suggestion that PAHO or this article endorse any specific organization or products. The use of the PAHO logo is not permitted. This notice should be preserved along with the article’s original URL. |
spellingShingle | Original Research Faccone, Diego Rapoport, Melina Albornoz, Ezequiel Celaya, Federico De Mendieta, Juan De Belder, Denise Lucero, Celeste Gomez, Sonia Danze, Diego Pasteran, Fernando Corso, Alejandra Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012–2018 |
title | Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012–2018 |
title_full | Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012–2018 |
title_fullStr | Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012–2018 |
title_full_unstemmed | Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012–2018 |
title_short | Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012–2018 |
title_sort | plasmidic resistance to colistin mediated by mcr-1 gene in escherichia coli clinical isolates in argentina: a retrospective study, 2012–2018 |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498280/ https://www.ncbi.nlm.nih.gov/pubmed/32973904 http://dx.doi.org/10.26633/RPSP.2020.55 |
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