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Regulation of Gene Activation by Competitive Cross Talking Pathways

The phenomenon of a gene expression burst has been attributed to random transitions between the active and inactive states of a gene. However, the mechanisms underlying regulation of the activation process in response to environmental changes remain unclear. Here, we model gene activation as a conse...

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Detalles Bibliográficos
Autores principales: Jiao, Feng, Zhu, Chunjuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499120/
https://www.ncbi.nlm.nih.gov/pubmed/32861266
http://dx.doi.org/10.1016/j.bpj.2020.08.011
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author Jiao, Feng
Zhu, Chunjuan
author_facet Jiao, Feng
Zhu, Chunjuan
author_sort Jiao, Feng
collection PubMed
description The phenomenon of a gene expression burst has been attributed to random transitions between the active and inactive states of a gene. However, the mechanisms underlying regulation of the activation process in response to environmental changes remain unclear. Here, we model gene activation as a consequence of the competitive cross talk between a weak basal pathway and an inducible signaling pathway and reveal rich expression dynamics along with intricate dependence of noise and Fano factor on mean expression levels. These theoretical results are in good agreement with a large experimental data set in Escherichia coli, yeast, and mammalian cells. Furthermore, both theoretical analyses and supporting biological evidence converge to demonstrate the existence of a tradeoff that governs the sharp up- and downregulation of gene expression, suggesting an ordered scenario that activates a gene under varying conditions. These regulation modes, together with cross talk pathways, may provide new guidance for the analysis and interpretation of genetic data in various applications, ranging from genetic engineering to therapeutic targets of disease.
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spelling pubmed-74991202020-10-10 Regulation of Gene Activation by Competitive Cross Talking Pathways Jiao, Feng Zhu, Chunjuan Biophys J Articles The phenomenon of a gene expression burst has been attributed to random transitions between the active and inactive states of a gene. However, the mechanisms underlying regulation of the activation process in response to environmental changes remain unclear. Here, we model gene activation as a consequence of the competitive cross talk between a weak basal pathway and an inducible signaling pathway and reveal rich expression dynamics along with intricate dependence of noise and Fano factor on mean expression levels. These theoretical results are in good agreement with a large experimental data set in Escherichia coli, yeast, and mammalian cells. Furthermore, both theoretical analyses and supporting biological evidence converge to demonstrate the existence of a tradeoff that governs the sharp up- and downregulation of gene expression, suggesting an ordered scenario that activates a gene under varying conditions. These regulation modes, together with cross talk pathways, may provide new guidance for the analysis and interpretation of genetic data in various applications, ranging from genetic engineering to therapeutic targets of disease. The Biophysical Society 2020-09-15 2020-08-19 /pmc/articles/PMC7499120/ /pubmed/32861266 http://dx.doi.org/10.1016/j.bpj.2020.08.011 Text en © 2020 Biophysical Society. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Articles
Jiao, Feng
Zhu, Chunjuan
Regulation of Gene Activation by Competitive Cross Talking Pathways
title Regulation of Gene Activation by Competitive Cross Talking Pathways
title_full Regulation of Gene Activation by Competitive Cross Talking Pathways
title_fullStr Regulation of Gene Activation by Competitive Cross Talking Pathways
title_full_unstemmed Regulation of Gene Activation by Competitive Cross Talking Pathways
title_short Regulation of Gene Activation by Competitive Cross Talking Pathways
title_sort regulation of gene activation by competitive cross talking pathways
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499120/
https://www.ncbi.nlm.nih.gov/pubmed/32861266
http://dx.doi.org/10.1016/j.bpj.2020.08.011
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