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Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks

Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass. However, the contribution of each hemicellulose type to the mechanical properties of secondary plant cell walls remains elusive. Here we homogen...

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Autores principales: Berglund, Jennie, Mikkelsen, Deirdre, Flanagan, Bernadine M., Dhital, Sushil, Gaunitz, Stefan, Henriksson, Gunnar, Lindström, Mikael E., Yakubov, Gleb E., Gidley, Michael J., Vilaplana, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499266/
https://www.ncbi.nlm.nih.gov/pubmed/32943624
http://dx.doi.org/10.1038/s41467-020-18390-z
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author Berglund, Jennie
Mikkelsen, Deirdre
Flanagan, Bernadine M.
Dhital, Sushil
Gaunitz, Stefan
Henriksson, Gunnar
Lindström, Mikael E.
Yakubov, Gleb E.
Gidley, Michael J.
Vilaplana, Francisco
author_facet Berglund, Jennie
Mikkelsen, Deirdre
Flanagan, Bernadine M.
Dhital, Sushil
Gaunitz, Stefan
Henriksson, Gunnar
Lindström, Mikael E.
Yakubov, Gleb E.
Gidley, Michael J.
Vilaplana, Francisco
author_sort Berglund, Jennie
collection PubMed
description Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass. However, the contribution of each hemicellulose type to the mechanical properties of secondary plant cell walls remains elusive. Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles. These composite hydrogels can be therefore envisioned as models of secondary plant cell walls prior to lignification. The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels. The wood hemicelluloses exhibit distinct biomechanical contributions, with glucomannans increasing the elastic modulus in compression, and xylans contributing to a dramatic increase of the elongation at break under tension. These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils. Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.
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spelling pubmed-74992662020-10-01 Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks Berglund, Jennie Mikkelsen, Deirdre Flanagan, Bernadine M. Dhital, Sushil Gaunitz, Stefan Henriksson, Gunnar Lindström, Mikael E. Yakubov, Gleb E. Gidley, Michael J. Vilaplana, Francisco Nat Commun Article Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass. However, the contribution of each hemicellulose type to the mechanical properties of secondary plant cell walls remains elusive. Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles. These composite hydrogels can be therefore envisioned as models of secondary plant cell walls prior to lignification. The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels. The wood hemicelluloses exhibit distinct biomechanical contributions, with glucomannans increasing the elastic modulus in compression, and xylans contributing to a dramatic increase of the elongation at break under tension. These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils. Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties. Nature Publishing Group UK 2020-09-17 /pmc/articles/PMC7499266/ /pubmed/32943624 http://dx.doi.org/10.1038/s41467-020-18390-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Berglund, Jennie
Mikkelsen, Deirdre
Flanagan, Bernadine M.
Dhital, Sushil
Gaunitz, Stefan
Henriksson, Gunnar
Lindström, Mikael E.
Yakubov, Gleb E.
Gidley, Michael J.
Vilaplana, Francisco
Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks
title Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks
title_full Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks
title_fullStr Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks
title_full_unstemmed Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks
title_short Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks
title_sort wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499266/
https://www.ncbi.nlm.nih.gov/pubmed/32943624
http://dx.doi.org/10.1038/s41467-020-18390-z
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