Cargando…

Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes

This study was aimed to evaluate the effect of mulberry leaf flavonoids (MLF) on oxidative stress, metabolic hormones, and milk production in Murrah buffaloes. Forty multiparous Murrah buffaloes (4 ± 1 lactations) with similar body weight (average 600 ± 50 Kg) and stage of lactation (90 ± 20 d) were...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Mengwei, Hassan, Faiz-ul, Tang, Zhenhua, Peng, Lijuan, Liang, Xin, Li, Lili, Peng, Kaiping, Xie, Fang, Yang, Chengjian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500204/
https://www.ncbi.nlm.nih.gov/pubmed/33102551
http://dx.doi.org/10.3389/fvets.2020.00599
_version_ 1783583819946786816
author Li, Mengwei
Hassan, Faiz-ul
Tang, Zhenhua
Peng, Lijuan
Liang, Xin
Li, Lili
Peng, Kaiping
Xie, Fang
Yang, Chengjian
author_facet Li, Mengwei
Hassan, Faiz-ul
Tang, Zhenhua
Peng, Lijuan
Liang, Xin
Li, Lili
Peng, Kaiping
Xie, Fang
Yang, Chengjian
author_sort Li, Mengwei
collection PubMed
description This study was aimed to evaluate the effect of mulberry leaf flavonoids (MLF) on oxidative stress, metabolic hormones, and milk production in Murrah buffaloes. Forty multiparous Murrah buffaloes (4 ± 1 lactations) with similar body weight (average 600 ± 50 Kg) and stage of lactation (90 ± 20 d) were randomly selected for this trial. Four treatment groups (10 buffaloes per group) with different doses of MLF included; control (0 g/d), MLF15 (15 g/d), MLF30 (30 g/d), and MLF45 (45 g/d). Buffaloes were fed with total mix ration consisting of grass (Pennisetum purpureum schum), brewery's grain and concentrate mixture for 5 weeks. Meteorological data including ambient temperature and relative humidity were recorded using the online dust monitoring system to calculate temperature-humidity index (THI). After 1 week of the adaptation, milk yield was recorded daily while physiological parameters (respiratory rate, rectal, and body surface temperature), and milk composition were measured weekly. At the end of the trial, blood samples were collected to analyze serum metabolic hormones including estradiol (E2), growth hormone (GH), prolactin (PRL), Tri-iodothyronine (T3), and Thyroxine (T4). Moreover, serum heat shock proteins (HSP), antioxidants enzymes including malondialdehyde (MDA), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) and blood biochemical indices were also analyzed. Results revealed a decrease (P = 0.012) in serum MDA level while increasing (P < 0.01) the HSP and serum GHS-Px contents in supplemented buffaloes. Treatment showed a linear and quadratic decrease (p = 0.001) in the serum T-AOC while reducing CAT contents linearly (p = 0.012) as compared to the control. However, no effect of treatment on serum SOD content was observed. Treatment resulted a linear increase (p = 0.001) in serum GH and PRL hormones while increasing serum E2 levels linearly (P < 0.001) and quadratically (P = 0.025). Treatment increased (p = 0.038) the daily milk yield as compared to the control. However, increase (P < 0.05) in serum T3 and T4 contents, fat corrected milk (4%) and milk protein (%) was observed only in MLF45. Moreover, we observed no change in serum biochemical indices except insulin which linearly increased (p = 0.002) in MLF45. Our findings indicated that MLF at 45 g per day is an appropriate level to enhance milk performance and alleviate heat stress in buffaloes.
format Online
Article
Text
id pubmed-7500204
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-75002042020-10-22 Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes Li, Mengwei Hassan, Faiz-ul Tang, Zhenhua Peng, Lijuan Liang, Xin Li, Lili Peng, Kaiping Xie, Fang Yang, Chengjian Front Vet Sci Veterinary Science This study was aimed to evaluate the effect of mulberry leaf flavonoids (MLF) on oxidative stress, metabolic hormones, and milk production in Murrah buffaloes. Forty multiparous Murrah buffaloes (4 ± 1 lactations) with similar body weight (average 600 ± 50 Kg) and stage of lactation (90 ± 20 d) were randomly selected for this trial. Four treatment groups (10 buffaloes per group) with different doses of MLF included; control (0 g/d), MLF15 (15 g/d), MLF30 (30 g/d), and MLF45 (45 g/d). Buffaloes were fed with total mix ration consisting of grass (Pennisetum purpureum schum), brewery's grain and concentrate mixture for 5 weeks. Meteorological data including ambient temperature and relative humidity were recorded using the online dust monitoring system to calculate temperature-humidity index (THI). After 1 week of the adaptation, milk yield was recorded daily while physiological parameters (respiratory rate, rectal, and body surface temperature), and milk composition were measured weekly. At the end of the trial, blood samples were collected to analyze serum metabolic hormones including estradiol (E2), growth hormone (GH), prolactin (PRL), Tri-iodothyronine (T3), and Thyroxine (T4). Moreover, serum heat shock proteins (HSP), antioxidants enzymes including malondialdehyde (MDA), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) and blood biochemical indices were also analyzed. Results revealed a decrease (P = 0.012) in serum MDA level while increasing (P < 0.01) the HSP and serum GHS-Px contents in supplemented buffaloes. Treatment showed a linear and quadratic decrease (p = 0.001) in the serum T-AOC while reducing CAT contents linearly (p = 0.012) as compared to the control. However, no effect of treatment on serum SOD content was observed. Treatment resulted a linear increase (p = 0.001) in serum GH and PRL hormones while increasing serum E2 levels linearly (P < 0.001) and quadratically (P = 0.025). Treatment increased (p = 0.038) the daily milk yield as compared to the control. However, increase (P < 0.05) in serum T3 and T4 contents, fat corrected milk (4%) and milk protein (%) was observed only in MLF45. Moreover, we observed no change in serum biochemical indices except insulin which linearly increased (p = 0.002) in MLF45. Our findings indicated that MLF at 45 g per day is an appropriate level to enhance milk performance and alleviate heat stress in buffaloes. Frontiers Media S.A. 2020-09-04 /pmc/articles/PMC7500204/ /pubmed/33102551 http://dx.doi.org/10.3389/fvets.2020.00599 Text en Copyright © 2020 Li, Hassan, Tang, Peng, Liang, Li, Peng, Xie and Yang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Li, Mengwei
Hassan, Faiz-ul
Tang, Zhenhua
Peng, Lijuan
Liang, Xin
Li, Lili
Peng, Kaiping
Xie, Fang
Yang, Chengjian
Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes
title Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes
title_full Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes
title_fullStr Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes
title_full_unstemmed Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes
title_short Mulberry Leaf Flavonoids Improve Milk Production, Antioxidant, and Metabolic Status of Water Buffaloes
title_sort mulberry leaf flavonoids improve milk production, antioxidant, and metabolic status of water buffaloes
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500204/
https://www.ncbi.nlm.nih.gov/pubmed/33102551
http://dx.doi.org/10.3389/fvets.2020.00599
work_keys_str_mv AT limengwei mulberryleafflavonoidsimprovemilkproductionantioxidantandmetabolicstatusofwaterbuffaloes
AT hassanfaizul mulberryleafflavonoidsimprovemilkproductionantioxidantandmetabolicstatusofwaterbuffaloes
AT tangzhenhua mulberryleafflavonoidsimprovemilkproductionantioxidantandmetabolicstatusofwaterbuffaloes
AT penglijuan mulberryleafflavonoidsimprovemilkproductionantioxidantandmetabolicstatusofwaterbuffaloes
AT liangxin mulberryleafflavonoidsimprovemilkproductionantioxidantandmetabolicstatusofwaterbuffaloes
AT lilili mulberryleafflavonoidsimprovemilkproductionantioxidantandmetabolicstatusofwaterbuffaloes
AT pengkaiping mulberryleafflavonoidsimprovemilkproductionantioxidantandmetabolicstatusofwaterbuffaloes
AT xiefang mulberryleafflavonoidsimprovemilkproductionantioxidantandmetabolicstatusofwaterbuffaloes
AT yangchengjian mulberryleafflavonoidsimprovemilkproductionantioxidantandmetabolicstatusofwaterbuffaloes