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miRNA‐216 knockdown has effects to suppress osteosarcoma via stimulating PTEN
The aim of this study is to explain the effects and mechanism of miRNA‐216 in osteosarcoma. We firstly evaluated the PTEN expression in 30 pairs of tumor and adjacent tissues which were from the 30 osteosarcoma patients. In the following cell experiments, we measured the cell proliferation, cell cyc...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500758/ https://www.ncbi.nlm.nih.gov/pubmed/32994932 http://dx.doi.org/10.1002/fsn3.1587 |
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author | Jiang, Ping Yang, Xin Li, Yuanli Chen, Juan |
author_facet | Jiang, Ping Yang, Xin Li, Yuanli Chen, Juan |
author_sort | Jiang, Ping |
collection | PubMed |
description | The aim of this study is to explain the effects and mechanism of miRNA‐216 in osteosarcoma. We firstly evaluated the PTEN expression in 30 pairs of tumor and adjacent tissues which were from the 30 osteosarcoma patients. In the following cell experiments, we measured the cell proliferation, cell cycle, cell invasion, and migration abilities of NC (normal control) group, BL (blank) group, siRNA (miRNA‐216 inhibitor) group, and siRNA+PTEN inhibitor group. Furthermore, we measured the relative protein expression of difference groups by WB to explain the mechanism of miRNA‐216 in osteosarcoma. The PTEN was confirmed the target gene of miRNA‐216 by double luciferase target test. In conclusion, miRNA‐216 was an oncogene in osteosarcoma. miRNA‐216 knockdown had effects to suppress cancer cell proliferation, invasion and migration and improve cell apoptosis by keeping in G1 phase via PTEN. |
format | Online Article Text |
id | pubmed-7500758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75007582020-09-28 miRNA‐216 knockdown has effects to suppress osteosarcoma via stimulating PTEN Jiang, Ping Yang, Xin Li, Yuanli Chen, Juan Food Sci Nutr Original Research The aim of this study is to explain the effects and mechanism of miRNA‐216 in osteosarcoma. We firstly evaluated the PTEN expression in 30 pairs of tumor and adjacent tissues which were from the 30 osteosarcoma patients. In the following cell experiments, we measured the cell proliferation, cell cycle, cell invasion, and migration abilities of NC (normal control) group, BL (blank) group, siRNA (miRNA‐216 inhibitor) group, and siRNA+PTEN inhibitor group. Furthermore, we measured the relative protein expression of difference groups by WB to explain the mechanism of miRNA‐216 in osteosarcoma. The PTEN was confirmed the target gene of miRNA‐216 by double luciferase target test. In conclusion, miRNA‐216 was an oncogene in osteosarcoma. miRNA‐216 knockdown had effects to suppress cancer cell proliferation, invasion and migration and improve cell apoptosis by keeping in G1 phase via PTEN. John Wiley and Sons Inc. 2020-08-09 /pmc/articles/PMC7500758/ /pubmed/32994932 http://dx.doi.org/10.1002/fsn3.1587 Text en © 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Jiang, Ping Yang, Xin Li, Yuanli Chen, Juan miRNA‐216 knockdown has effects to suppress osteosarcoma via stimulating PTEN |
title | miRNA‐216 knockdown has effects to suppress osteosarcoma via stimulating PTEN |
title_full | miRNA‐216 knockdown has effects to suppress osteosarcoma via stimulating PTEN |
title_fullStr | miRNA‐216 knockdown has effects to suppress osteosarcoma via stimulating PTEN |
title_full_unstemmed | miRNA‐216 knockdown has effects to suppress osteosarcoma via stimulating PTEN |
title_short | miRNA‐216 knockdown has effects to suppress osteosarcoma via stimulating PTEN |
title_sort | mirna‐216 knockdown has effects to suppress osteosarcoma via stimulating pten |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500758/ https://www.ncbi.nlm.nih.gov/pubmed/32994932 http://dx.doi.org/10.1002/fsn3.1587 |
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