Cargando…

Preferred crystallographic orientation of cellulose in plant primary cell walls

Cellulose, the most abundant biopolymer on earth, is a versatile, energy rich material found in the cell walls of plants, bacteria, algae, and tunicates. It is well established that cellulose is crystalline, although the orientational order of cellulose crystallites normal to the plane of the cell w...

Descripción completa

Detalles Bibliográficos
Autores principales: Ye, Dan, Rongpipi, Sintu, Kiemle, Sarah N., Barnes, William J., Chaves, Arielle M., Zhu, Chenhui, Norman, Victoria A., Liebman-Peláez, Alexander, Hexemer, Alexander, Toney, Michael F., Roberts, Alison W., Anderson, Charles T., Cosgrove, Daniel J., Gomez, Esther W., Gomez, Enrique D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501228/
https://www.ncbi.nlm.nih.gov/pubmed/32948753
http://dx.doi.org/10.1038/s41467-020-18449-x
_version_ 1783583997791567872
author Ye, Dan
Rongpipi, Sintu
Kiemle, Sarah N.
Barnes, William J.
Chaves, Arielle M.
Zhu, Chenhui
Norman, Victoria A.
Liebman-Peláez, Alexander
Hexemer, Alexander
Toney, Michael F.
Roberts, Alison W.
Anderson, Charles T.
Cosgrove, Daniel J.
Gomez, Esther W.
Gomez, Enrique D.
author_facet Ye, Dan
Rongpipi, Sintu
Kiemle, Sarah N.
Barnes, William J.
Chaves, Arielle M.
Zhu, Chenhui
Norman, Victoria A.
Liebman-Peláez, Alexander
Hexemer, Alexander
Toney, Michael F.
Roberts, Alison W.
Anderson, Charles T.
Cosgrove, Daniel J.
Gomez, Esther W.
Gomez, Enrique D.
author_sort Ye, Dan
collection PubMed
description Cellulose, the most abundant biopolymer on earth, is a versatile, energy rich material found in the cell walls of plants, bacteria, algae, and tunicates. It is well established that cellulose is crystalline, although the orientational order of cellulose crystallites normal to the plane of the cell wall has not been characterized. A preferred orientational alignment of cellulose crystals could be an important determinant of the mechanical properties of the cell wall and of cellulose-cellulose and cellulose-matrix interactions. Here, the crystalline structures of cellulose in primary cell walls of onion (Allium cepa), the model eudicot Arabidopsis (Arabidopsis thaliana), and moss (Physcomitrella patens) were examined through grazing incidence wide angle X-ray scattering (GIWAXS). We find that GIWAXS can decouple diffraction from cellulose and epicuticular wax crystals in cell walls. Pole figures constructed from a combination of GIWAXS and X-ray rocking scans reveal that cellulose crystals have a preferred crystallographic orientation with the (200) and (110)/([Formula: see text] ) planes preferentially stacked parallel to the cell wall. This orientational ordering of cellulose crystals, termed texturing in materials science, represents a previously unreported measure of cellulose organization and contradicts the predominant hypothesis of twisting of microfibrils in plant primary cell walls.
format Online
Article
Text
id pubmed-7501228
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-75012282020-10-01 Preferred crystallographic orientation of cellulose in plant primary cell walls Ye, Dan Rongpipi, Sintu Kiemle, Sarah N. Barnes, William J. Chaves, Arielle M. Zhu, Chenhui Norman, Victoria A. Liebman-Peláez, Alexander Hexemer, Alexander Toney, Michael F. Roberts, Alison W. Anderson, Charles T. Cosgrove, Daniel J. Gomez, Esther W. Gomez, Enrique D. Nat Commun Article Cellulose, the most abundant biopolymer on earth, is a versatile, energy rich material found in the cell walls of plants, bacteria, algae, and tunicates. It is well established that cellulose is crystalline, although the orientational order of cellulose crystallites normal to the plane of the cell wall has not been characterized. A preferred orientational alignment of cellulose crystals could be an important determinant of the mechanical properties of the cell wall and of cellulose-cellulose and cellulose-matrix interactions. Here, the crystalline structures of cellulose in primary cell walls of onion (Allium cepa), the model eudicot Arabidopsis (Arabidopsis thaliana), and moss (Physcomitrella patens) were examined through grazing incidence wide angle X-ray scattering (GIWAXS). We find that GIWAXS can decouple diffraction from cellulose and epicuticular wax crystals in cell walls. Pole figures constructed from a combination of GIWAXS and X-ray rocking scans reveal that cellulose crystals have a preferred crystallographic orientation with the (200) and (110)/([Formula: see text] ) planes preferentially stacked parallel to the cell wall. This orientational ordering of cellulose crystals, termed texturing in materials science, represents a previously unreported measure of cellulose organization and contradicts the predominant hypothesis of twisting of microfibrils in plant primary cell walls. Nature Publishing Group UK 2020-09-18 /pmc/articles/PMC7501228/ /pubmed/32948753 http://dx.doi.org/10.1038/s41467-020-18449-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ye, Dan
Rongpipi, Sintu
Kiemle, Sarah N.
Barnes, William J.
Chaves, Arielle M.
Zhu, Chenhui
Norman, Victoria A.
Liebman-Peláez, Alexander
Hexemer, Alexander
Toney, Michael F.
Roberts, Alison W.
Anderson, Charles T.
Cosgrove, Daniel J.
Gomez, Esther W.
Gomez, Enrique D.
Preferred crystallographic orientation of cellulose in plant primary cell walls
title Preferred crystallographic orientation of cellulose in plant primary cell walls
title_full Preferred crystallographic orientation of cellulose in plant primary cell walls
title_fullStr Preferred crystallographic orientation of cellulose in plant primary cell walls
title_full_unstemmed Preferred crystallographic orientation of cellulose in plant primary cell walls
title_short Preferred crystallographic orientation of cellulose in plant primary cell walls
title_sort preferred crystallographic orientation of cellulose in plant primary cell walls
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501228/
https://www.ncbi.nlm.nih.gov/pubmed/32948753
http://dx.doi.org/10.1038/s41467-020-18449-x
work_keys_str_mv AT yedan preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT rongpipisintu preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT kiemlesarahn preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT barneswilliamj preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT chavesariellem preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT zhuchenhui preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT normanvictoriaa preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT liebmanpelaezalexander preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT hexemeralexander preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT toneymichaelf preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT robertsalisonw preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT andersoncharlest preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT cosgrovedanielj preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT gomezestherw preferredcrystallographicorientationofcelluloseinplantprimarycellwalls
AT gomezenriqued preferredcrystallographicorientationofcelluloseinplantprimarycellwalls