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Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease

Gene expression profiles of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) were evaluated in peripheral blood leukocytes of children with nonalcoholic fatty liver disease (NAFLD). Gene expression patterns were correlated with their plasma protein counterparts, systemic paramete...

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Autores principales: Trojanek, Joanna B., Michałkiewicz, Jacek, Grzywa-Czuba, Renata, Jańczyk, Wojciech, Gackowska, Lidia, Kubiszewska, Izabela, Helmin-Basa, Anna, Wierzbicka-Rucińska, Aldona, Szalecki, Mieczysław, Socha, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501567/
https://www.ncbi.nlm.nih.gov/pubmed/32963496
http://dx.doi.org/10.1155/2020/8327945
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author Trojanek, Joanna B.
Michałkiewicz, Jacek
Grzywa-Czuba, Renata
Jańczyk, Wojciech
Gackowska, Lidia
Kubiszewska, Izabela
Helmin-Basa, Anna
Wierzbicka-Rucińska, Aldona
Szalecki, Mieczysław
Socha, Piotr
author_facet Trojanek, Joanna B.
Michałkiewicz, Jacek
Grzywa-Czuba, Renata
Jańczyk, Wojciech
Gackowska, Lidia
Kubiszewska, Izabela
Helmin-Basa, Anna
Wierzbicka-Rucińska, Aldona
Szalecki, Mieczysław
Socha, Piotr
author_sort Trojanek, Joanna B.
collection PubMed
description Gene expression profiles of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) were evaluated in peripheral blood leukocytes of children with nonalcoholic fatty liver disease (NAFLD). Gene expression patterns were correlated with their plasma protein counterparts, systemic parameters of liver injury, and selected markers of inflammation. The MMP-2, MMP-9, MMP-12, MMP-14, TIMP-1, TIMP-2, TGF-β, and IL-6 transcripts levels were tested by the real-time PCR. Plasma concentrations of MMP-9, TIMP-1, MMP-9/TIMP-1 ratio, MMP-2/TIMP-2 ratio, sCD14, leptin, resistin, IL-1 beta, and IL-6 and serum markers of liver injury were estimated by ELISA. The MMP-9, TIMP-2 expression levels, plasma amounts of MMP-9, TIMP-1, and the MMP-9/TIMP-1 ratio were increased in children with NAFLD. Concentrations of AST, ALT, GGT, and leptin were elevated in serum patients with NAFLD, while concentration of other inflammatory or liver injury markers was unchanged. The MMP-2 and MMP-9 levels correlated with serum liver injury parameters (ALT and GGT concentrations, respectively); there were no other correlations between MMP/TIMP gene expression profiles, their plasma counterparts, and serum inflammatory markers. Association of MMP-2 and MMP-9 expression with serum liver injury parameters (ALT, GGT) may suggest leukocyte engagement in the early stages of NAFLD development which possibly precedes subsequent systemic inflammatory responses.
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spelling pubmed-75015672020-09-21 Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease Trojanek, Joanna B. Michałkiewicz, Jacek Grzywa-Czuba, Renata Jańczyk, Wojciech Gackowska, Lidia Kubiszewska, Izabela Helmin-Basa, Anna Wierzbicka-Rucińska, Aldona Szalecki, Mieczysław Socha, Piotr Mediators Inflamm Research Article Gene expression profiles of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) were evaluated in peripheral blood leukocytes of children with nonalcoholic fatty liver disease (NAFLD). Gene expression patterns were correlated with their plasma protein counterparts, systemic parameters of liver injury, and selected markers of inflammation. The MMP-2, MMP-9, MMP-12, MMP-14, TIMP-1, TIMP-2, TGF-β, and IL-6 transcripts levels were tested by the real-time PCR. Plasma concentrations of MMP-9, TIMP-1, MMP-9/TIMP-1 ratio, MMP-2/TIMP-2 ratio, sCD14, leptin, resistin, IL-1 beta, and IL-6 and serum markers of liver injury were estimated by ELISA. The MMP-9, TIMP-2 expression levels, plasma amounts of MMP-9, TIMP-1, and the MMP-9/TIMP-1 ratio were increased in children with NAFLD. Concentrations of AST, ALT, GGT, and leptin were elevated in serum patients with NAFLD, while concentration of other inflammatory or liver injury markers was unchanged. The MMP-2 and MMP-9 levels correlated with serum liver injury parameters (ALT and GGT concentrations, respectively); there were no other correlations between MMP/TIMP gene expression profiles, their plasma counterparts, and serum inflammatory markers. Association of MMP-2 and MMP-9 expression with serum liver injury parameters (ALT, GGT) may suggest leukocyte engagement in the early stages of NAFLD development which possibly precedes subsequent systemic inflammatory responses. Hindawi 2020-09-10 /pmc/articles/PMC7501567/ /pubmed/32963496 http://dx.doi.org/10.1155/2020/8327945 Text en Copyright © 2020 Joanna B. Trojanek et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Trojanek, Joanna B.
Michałkiewicz, Jacek
Grzywa-Czuba, Renata
Jańczyk, Wojciech
Gackowska, Lidia
Kubiszewska, Izabela
Helmin-Basa, Anna
Wierzbicka-Rucińska, Aldona
Szalecki, Mieczysław
Socha, Piotr
Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease
title Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease
title_full Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease
title_fullStr Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease
title_full_unstemmed Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease
title_short Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease
title_sort expression of matrix metalloproteinases and their tissue inhibitors in peripheral blood leukocytes and plasma of children with nonalcoholic fatty liver disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501567/
https://www.ncbi.nlm.nih.gov/pubmed/32963496
http://dx.doi.org/10.1155/2020/8327945
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