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Kappa opioid receptors in the central amygdala modulate spinal nociceptive processing through an action on amygdala CRF neurons

The amygdala plays an important role in the emotional-affective aspects of behaviors and pain, but can also modulate sensory aspect of pain (“nociception”), likely through coupling to descending modulatory systems. Here we explored the functional coupling of the amygdala to spinal nociception. We fo...

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Autores principales: Ji, Guangchen, Neugebauer, Volker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501648/
https://www.ncbi.nlm.nih.gov/pubmed/32948219
http://dx.doi.org/10.1186/s13041-020-00669-3
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author Ji, Guangchen
Neugebauer, Volker
author_facet Ji, Guangchen
Neugebauer, Volker
author_sort Ji, Guangchen
collection PubMed
description The amygdala plays an important role in the emotional-affective aspects of behaviors and pain, but can also modulate sensory aspect of pain (“nociception”), likely through coupling to descending modulatory systems. Here we explored the functional coupling of the amygdala to spinal nociception. We found that pharmacological activation of neurons in the central nucleus of the amygdala (CeA) increased the activity of spinal dorsal horn neurons; and this effect was blocked by optogenetic silencing of corticotropin releasing factor (CRF) positive CeA neurons. A kappa opioid receptor (KOR) agonist (U-69,593) was administered into the CeA by microdialysis. KOR was targeted because of their role in averse-affective behaviors through actions in limbic brain regions. Extracellular single-unit recordings were made of CeA neurons or spinal dorsal horn neurons in anesthetized transgenic Crh-Cre rats. Neurons responded more strongly to noxious than innocuous stimuli. U-69,593 increased the responses of CeA and spinal neurons to innocuous and noxious mechanical stimulation of peripheral tissues. The facilitatory effect of the agonist was blocked by optical silencing of CRF-CeA neurons though light activation of halorhodopsin expressed in these neurons by viral-vector. The CRF system in the amygdala has been implicated in aversiveness and pain modulation. The results suggest that the amygdala can modulate spinal nociceptive processing in a positive direction through CRF-CeA neurons and that KOR activation in the amygdala (CeA) has pro-nociceptive effects.
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spelling pubmed-75016482020-09-22 Kappa opioid receptors in the central amygdala modulate spinal nociceptive processing through an action on amygdala CRF neurons Ji, Guangchen Neugebauer, Volker Mol Brain Short Report The amygdala plays an important role in the emotional-affective aspects of behaviors and pain, but can also modulate sensory aspect of pain (“nociception”), likely through coupling to descending modulatory systems. Here we explored the functional coupling of the amygdala to spinal nociception. We found that pharmacological activation of neurons in the central nucleus of the amygdala (CeA) increased the activity of spinal dorsal horn neurons; and this effect was blocked by optogenetic silencing of corticotropin releasing factor (CRF) positive CeA neurons. A kappa opioid receptor (KOR) agonist (U-69,593) was administered into the CeA by microdialysis. KOR was targeted because of their role in averse-affective behaviors through actions in limbic brain regions. Extracellular single-unit recordings were made of CeA neurons or spinal dorsal horn neurons in anesthetized transgenic Crh-Cre rats. Neurons responded more strongly to noxious than innocuous stimuli. U-69,593 increased the responses of CeA and spinal neurons to innocuous and noxious mechanical stimulation of peripheral tissues. The facilitatory effect of the agonist was blocked by optical silencing of CRF-CeA neurons though light activation of halorhodopsin expressed in these neurons by viral-vector. The CRF system in the amygdala has been implicated in aversiveness and pain modulation. The results suggest that the amygdala can modulate spinal nociceptive processing in a positive direction through CRF-CeA neurons and that KOR activation in the amygdala (CeA) has pro-nociceptive effects. BioMed Central 2020-09-18 /pmc/articles/PMC7501648/ /pubmed/32948219 http://dx.doi.org/10.1186/s13041-020-00669-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Ji, Guangchen
Neugebauer, Volker
Kappa opioid receptors in the central amygdala modulate spinal nociceptive processing through an action on amygdala CRF neurons
title Kappa opioid receptors in the central amygdala modulate spinal nociceptive processing through an action on amygdala CRF neurons
title_full Kappa opioid receptors in the central amygdala modulate spinal nociceptive processing through an action on amygdala CRF neurons
title_fullStr Kappa opioid receptors in the central amygdala modulate spinal nociceptive processing through an action on amygdala CRF neurons
title_full_unstemmed Kappa opioid receptors in the central amygdala modulate spinal nociceptive processing through an action on amygdala CRF neurons
title_short Kappa opioid receptors in the central amygdala modulate spinal nociceptive processing through an action on amygdala CRF neurons
title_sort kappa opioid receptors in the central amygdala modulate spinal nociceptive processing through an action on amygdala crf neurons
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501648/
https://www.ncbi.nlm.nih.gov/pubmed/32948219
http://dx.doi.org/10.1186/s13041-020-00669-3
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