Protocol for Efficient Protein Synthesis Detection by Click Chemistry in Colorectal Cancer Patient-Derived Organoids Grown In Vitro

Here, we describe a protocol to detect and visualize protein synthesis by click-chemistry-based immunofluorescence in patient-derived organoids (PDOs) in vitro. The protocol uses O-propargyl puromycin (OPP), an analog of puromycin that enters the acceptor site of ribosomes and is incorporated into n...

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Detalles Bibliográficos
Autores principales: Morral, Clara, Stanisavljevic, Jelena, Batlle, Eduard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501725/
https://www.ncbi.nlm.nih.gov/pubmed/33000005
http://dx.doi.org/10.1016/j.xpro.2020.100103

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