Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling

Interstitial cells have a crucial role in cardiac fibrosis and repair of the mammalian heart. Single-cell profiling using droplet-based technology has revolutionized the investigation of cell states and identities. Here, we present a protocol for the efficient isolation of high-quality live nucleate...

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Detalles Bibliográficos
Autores principales: Forte, Elvira, Daigle, Sandra, Rosenthal, Nadia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501728/
https://www.ncbi.nlm.nih.gov/pubmed/33000003
http://dx.doi.org/10.1016/j.xpro.2020.100077
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author Forte, Elvira
Daigle, Sandra
Rosenthal, Nadia A.
author_facet Forte, Elvira
Daigle, Sandra
Rosenthal, Nadia A.
author_sort Forte, Elvira
collection PubMed
description Interstitial cells have a crucial role in cardiac fibrosis and repair of the mammalian heart. Single-cell profiling using droplet-based technology has revolutionized the investigation of cell states and identities. Here, we present a protocol for the efficient isolation of high-quality live nucleated non-cardiomyocytes from adult murine heart, for unbiased single-cell RNA sequencing using 10× Chromium technology. This protocol has been applied to homeostatic and injured hearts from different mouse strains. For complete details on the use and execution of this protocol, please refer to Forte et al. (2020).
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spelling pubmed-75017282020-09-28 Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling Forte, Elvira Daigle, Sandra Rosenthal, Nadia A. STAR Protoc Protocol Interstitial cells have a crucial role in cardiac fibrosis and repair of the mammalian heart. Single-cell profiling using droplet-based technology has revolutionized the investigation of cell states and identities. Here, we present a protocol for the efficient isolation of high-quality live nucleated non-cardiomyocytes from adult murine heart, for unbiased single-cell RNA sequencing using 10× Chromium technology. This protocol has been applied to homeostatic and injured hearts from different mouse strains. For complete details on the use and execution of this protocol, please refer to Forte et al. (2020). Elsevier 2020-08-01 /pmc/articles/PMC7501728/ /pubmed/33000003 http://dx.doi.org/10.1016/j.xpro.2020.100077 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Forte, Elvira
Daigle, Sandra
Rosenthal, Nadia A.
Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling
title Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling
title_full Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling
title_fullStr Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling
title_full_unstemmed Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling
title_short Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling
title_sort protocol for isolation of cardiac interstitial cells from adult murine hearts for unbiased single cell profiling
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501728/
https://www.ncbi.nlm.nih.gov/pubmed/33000003
http://dx.doi.org/10.1016/j.xpro.2020.100077
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