Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling

Interstitial cells have a crucial role in cardiac fibrosis and repair of the mammalian heart. Single-cell profiling using droplet-based technology has revolutionized the investigation of cell states and identities. Here, we present a protocol for the efficient isolation of high-quality live nucleate...

Descripción completa

Detalles Bibliográficos
Autores principales: Forte, Elvira, Daigle, Sandra, Rosenthal, Nadia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501728/
https://www.ncbi.nlm.nih.gov/pubmed/33000003
http://dx.doi.org/10.1016/j.xpro.2020.100077

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501728/
https://www.ncbi.nlm.nih.gov/pubmed/33000003
http://dx.doi.org/10.1016/j.xpro.2020.100077

Ejemplares similares