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Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling
Interstitial cells have a crucial role in cardiac fibrosis and repair of the mammalian heart. Single-cell profiling using droplet-based technology has revolutionized the investigation of cell states and identities. Here, we present a protocol for the efficient isolation of high-quality live nucleate...
Autores principales: | Forte, Elvira, Daigle, Sandra, Rosenthal, Nadia A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501728/ https://www.ncbi.nlm.nih.gov/pubmed/33000003 http://dx.doi.org/10.1016/j.xpro.2020.100077 |
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