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BIOMASS YIELD 1 regulates sorghum biomass and grain yield via the shikimate pathway

Biomass and grain yield are key agronomic traits in sorghum (Sorghum bicolor); however, the molecular mechanisms that regulate these traits are not well understood. Here, we characterized the biomass yield 1 (by1) mutant, which displays a dramatically altered phenotype that includes reduced plant he...

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Autores principales: Chen, Jun, Zhu, Mengjiao, Liu, Ruixiang, Zhang, Meijing, Lv, Ya, Liu, Yishan, Xiao, Xin, Yuan, Jianhua, Cai, Hongwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501818/
https://www.ncbi.nlm.nih.gov/pubmed/32497182
http://dx.doi.org/10.1093/jxb/eraa275
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author Chen, Jun
Zhu, Mengjiao
Liu, Ruixiang
Zhang, Meijing
Lv, Ya
Liu, Yishan
Xiao, Xin
Yuan, Jianhua
Cai, Hongwei
author_facet Chen, Jun
Zhu, Mengjiao
Liu, Ruixiang
Zhang, Meijing
Lv, Ya
Liu, Yishan
Xiao, Xin
Yuan, Jianhua
Cai, Hongwei
author_sort Chen, Jun
collection PubMed
description Biomass and grain yield are key agronomic traits in sorghum (Sorghum bicolor); however, the molecular mechanisms that regulate these traits are not well understood. Here, we characterized the biomass yield 1 (by1) mutant, which displays a dramatically altered phenotype that includes reduced plant height, narrow stems, erect and narrow leaves, and abnormal floral organs. Histological analysis suggested that these phenotypic defects are mainly caused by inhibited cell elongation and abnormal floral organ development. Map-based cloning revealed that BY1 encodes a 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS) that catalyses the first step of the shikimate pathway. BY1 was localized in chloroplasts and was ubiquitously distributed in the organs examined, particularly in the roots, stems, leaves, and panicles, which was consistent with its role in biomass production and grain yield. Transcriptome analysis and metabolic profiling revealed that BY1 was involved in primary metabolism and that it affected the biosynthesis of various secondary metabolites, especially flavonoids. Taken together, these findings demonstrate that BY1 affects biomass and grain yield in sorghum by regulating primary and secondary metabolism via the shikimate pathway. Moreover, our results provide important insights into the relationship between plant development and metabolism.
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spelling pubmed-75018182020-09-23 BIOMASS YIELD 1 regulates sorghum biomass and grain yield via the shikimate pathway Chen, Jun Zhu, Mengjiao Liu, Ruixiang Zhang, Meijing Lv, Ya Liu, Yishan Xiao, Xin Yuan, Jianhua Cai, Hongwei J Exp Bot Research Papers Biomass and grain yield are key agronomic traits in sorghum (Sorghum bicolor); however, the molecular mechanisms that regulate these traits are not well understood. Here, we characterized the biomass yield 1 (by1) mutant, which displays a dramatically altered phenotype that includes reduced plant height, narrow stems, erect and narrow leaves, and abnormal floral organs. Histological analysis suggested that these phenotypic defects are mainly caused by inhibited cell elongation and abnormal floral organ development. Map-based cloning revealed that BY1 encodes a 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS) that catalyses the first step of the shikimate pathway. BY1 was localized in chloroplasts and was ubiquitously distributed in the organs examined, particularly in the roots, stems, leaves, and panicles, which was consistent with its role in biomass production and grain yield. Transcriptome analysis and metabolic profiling revealed that BY1 was involved in primary metabolism and that it affected the biosynthesis of various secondary metabolites, especially flavonoids. Taken together, these findings demonstrate that BY1 affects biomass and grain yield in sorghum by regulating primary and secondary metabolism via the shikimate pathway. Moreover, our results provide important insights into the relationship between plant development and metabolism. Oxford University Press 2020-06-04 /pmc/articles/PMC7501818/ /pubmed/32497182 http://dx.doi.org/10.1093/jxb/eraa275 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Chen, Jun
Zhu, Mengjiao
Liu, Ruixiang
Zhang, Meijing
Lv, Ya
Liu, Yishan
Xiao, Xin
Yuan, Jianhua
Cai, Hongwei
BIOMASS YIELD 1 regulates sorghum biomass and grain yield via the shikimate pathway
title BIOMASS YIELD 1 regulates sorghum biomass and grain yield via the shikimate pathway
title_full BIOMASS YIELD 1 regulates sorghum biomass and grain yield via the shikimate pathway
title_fullStr BIOMASS YIELD 1 regulates sorghum biomass and grain yield via the shikimate pathway
title_full_unstemmed BIOMASS YIELD 1 regulates sorghum biomass and grain yield via the shikimate pathway
title_short BIOMASS YIELD 1 regulates sorghum biomass and grain yield via the shikimate pathway
title_sort biomass yield 1 regulates sorghum biomass and grain yield via the shikimate pathway
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501818/
https://www.ncbi.nlm.nih.gov/pubmed/32497182
http://dx.doi.org/10.1093/jxb/eraa275
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