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Promoter methylation and expression pattern of DLX3, ATF4, and FRA1 genes during osteoblastic differentiation of adipose-derived mesenchymal stem cells

[Image: see text] Introduction: Nowadays, mesenchymal stem cells are touted as suitable cell supply for the restoration of injured bone tissue. The existence of osteogenic differentiation makes these cells capable of replenishing damaged cells in the least possible time. It has been shown that epige...

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Autores principales: Rahimzadeh, Sevda, Rahbarghazi, Reza, Aslani, Somayeh, Rajabi, Hadi, Latifi, Zeinab, Farshdousti Hagh, Majid, Nourazarian, Alireza, Nozad Charoudeh, Hojjatollah, Nouri, Mohammad, Abhari, Alireza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7502906/
https://www.ncbi.nlm.nih.gov/pubmed/32983940
http://dx.doi.org/10.34172/bi.2020.31
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author Rahimzadeh, Sevda
Rahbarghazi, Reza
Aslani, Somayeh
Rajabi, Hadi
Latifi, Zeinab
Farshdousti Hagh, Majid
Nourazarian, Alireza
Nozad Charoudeh, Hojjatollah
Nouri, Mohammad
Abhari, Alireza
author_facet Rahimzadeh, Sevda
Rahbarghazi, Reza
Aslani, Somayeh
Rajabi, Hadi
Latifi, Zeinab
Farshdousti Hagh, Majid
Nourazarian, Alireza
Nozad Charoudeh, Hojjatollah
Nouri, Mohammad
Abhari, Alireza
author_sort Rahimzadeh, Sevda
collection PubMed
description [Image: see text] Introduction: Nowadays, mesenchymal stem cells are touted as suitable cell supply for the restoration of injured bone tissue. The existence of osteogenic differentiation makes these cells capable of replenishing damaged cells in the least possible time. It has been shown that epigenetic modifications, especially DNA methylation, contribute to the regulation of various transcription factors during phenotype acquisition. Hence, we concentrated on the correlation between the promoter methylation and the expression of genes DLX3, ATF4 , and FRA1 during osteoblastic differentiation of adipose-derived mesenchymal stem cells in vitro after 21 days. Methods: Adipose-derived mesenchymal stem cells were cultured in osteogenesis differentiation medium supplemented with 0.1 µM dexamethasone, 10 mM β-glycerol phosphate, and 50 µM ascorbate-2-phosphate for 21 days. RNA and DNA extraction was done on days 0, 7, 14, and 21. Promoter methylation and expression levels of genes DLX3 , ATF4 , and FRA1 were analyzed by methylation-specific quantitative PCR and real-time PCR assays, respectively. Results: We found an upward expression trend with the increasing time for genes DLX3, ATF4, and FRA1 in stem cells committed to osteoblast-like lineage compared to the control group (P <0.05). On the contrary, methylation-specific quantitative PCR displayed decreased methylation rates of DLX3 and ATF4 genes, but not FRA1 , over time compared to the non-treated control cells (P <0.05). Bright-field images exhibited red-colored calcified deposits around Alizarin Red S-stained cells after 21 days compared to the control group. Statistical analysis showed a strong correlation between the transcription of genes DLX3 and ATF4 and methylation rate (P <0.05). Conclusion: In particular, osteoblastic differentiation of adipose-derived mesenchymal stem cells enhances DLX3 and ATF4 transcriptions by reducing methylation rate for 21 days.
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spelling pubmed-75029062020-09-25 Promoter methylation and expression pattern of DLX3, ATF4, and FRA1 genes during osteoblastic differentiation of adipose-derived mesenchymal stem cells Rahimzadeh, Sevda Rahbarghazi, Reza Aslani, Somayeh Rajabi, Hadi Latifi, Zeinab Farshdousti Hagh, Majid Nourazarian, Alireza Nozad Charoudeh, Hojjatollah Nouri, Mohammad Abhari, Alireza Bioimpacts Original Research [Image: see text] Introduction: Nowadays, mesenchymal stem cells are touted as suitable cell supply for the restoration of injured bone tissue. The existence of osteogenic differentiation makes these cells capable of replenishing damaged cells in the least possible time. It has been shown that epigenetic modifications, especially DNA methylation, contribute to the regulation of various transcription factors during phenotype acquisition. Hence, we concentrated on the correlation between the promoter methylation and the expression of genes DLX3, ATF4 , and FRA1 during osteoblastic differentiation of adipose-derived mesenchymal stem cells in vitro after 21 days. Methods: Adipose-derived mesenchymal stem cells were cultured in osteogenesis differentiation medium supplemented with 0.1 µM dexamethasone, 10 mM β-glycerol phosphate, and 50 µM ascorbate-2-phosphate for 21 days. RNA and DNA extraction was done on days 0, 7, 14, and 21. Promoter methylation and expression levels of genes DLX3 , ATF4 , and FRA1 were analyzed by methylation-specific quantitative PCR and real-time PCR assays, respectively. Results: We found an upward expression trend with the increasing time for genes DLX3, ATF4, and FRA1 in stem cells committed to osteoblast-like lineage compared to the control group (P <0.05). On the contrary, methylation-specific quantitative PCR displayed decreased methylation rates of DLX3 and ATF4 genes, but not FRA1 , over time compared to the non-treated control cells (P <0.05). Bright-field images exhibited red-colored calcified deposits around Alizarin Red S-stained cells after 21 days compared to the control group. Statistical analysis showed a strong correlation between the transcription of genes DLX3 and ATF4 and methylation rate (P <0.05). Conclusion: In particular, osteoblastic differentiation of adipose-derived mesenchymal stem cells enhances DLX3 and ATF4 transcriptions by reducing methylation rate for 21 days. Tabriz University of Medical Sciences 2020 2019-11-25 /pmc/articles/PMC7502906/ /pubmed/32983940 http://dx.doi.org/10.34172/bi.2020.31 Text en © 2020 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Research
Rahimzadeh, Sevda
Rahbarghazi, Reza
Aslani, Somayeh
Rajabi, Hadi
Latifi, Zeinab
Farshdousti Hagh, Majid
Nourazarian, Alireza
Nozad Charoudeh, Hojjatollah
Nouri, Mohammad
Abhari, Alireza
Promoter methylation and expression pattern of DLX3, ATF4, and FRA1 genes during osteoblastic differentiation of adipose-derived mesenchymal stem cells
title Promoter methylation and expression pattern of DLX3, ATF4, and FRA1 genes during osteoblastic differentiation of adipose-derived mesenchymal stem cells
title_full Promoter methylation and expression pattern of DLX3, ATF4, and FRA1 genes during osteoblastic differentiation of adipose-derived mesenchymal stem cells
title_fullStr Promoter methylation and expression pattern of DLX3, ATF4, and FRA1 genes during osteoblastic differentiation of adipose-derived mesenchymal stem cells
title_full_unstemmed Promoter methylation and expression pattern of DLX3, ATF4, and FRA1 genes during osteoblastic differentiation of adipose-derived mesenchymal stem cells
title_short Promoter methylation and expression pattern of DLX3, ATF4, and FRA1 genes during osteoblastic differentiation of adipose-derived mesenchymal stem cells
title_sort promoter methylation and expression pattern of dlx3, atf4, and fra1 genes during osteoblastic differentiation of adipose-derived mesenchymal stem cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7502906/
https://www.ncbi.nlm.nih.gov/pubmed/32983940
http://dx.doi.org/10.34172/bi.2020.31
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