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Immunomodulation and Generation of Tolerogenic Dendritic Cells by Probiotic Bacteria in Patients with Inflammatory Bowel Disease

In inflammatory bowel diseases (IBD), the therapeutic benefit and mucosal healing from specific probiotics may relate to the modulation of dendritic cells (DCs). Herein, we assessed the immunomodulatory effects of four probiotic strains including Lactobacillus salivarius, Bifidobacterium bifidum, Ba...

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Autores principales: Ghavami, Shaghayegh Baradaran, Yadegar, Abbas, Aghdaei, Hamid Asadzadeh, Sorrentino, Dario, Farmani, Maryam, Mir, Adil Shamim, Azimirad, Masoumeh, Balaii, Hedieh, Shahrokh, Shabnam, Zali, Mohammad Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503552/
https://www.ncbi.nlm.nih.gov/pubmed/32872480
http://dx.doi.org/10.3390/ijms21176266
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author Ghavami, Shaghayegh Baradaran
Yadegar, Abbas
Aghdaei, Hamid Asadzadeh
Sorrentino, Dario
Farmani, Maryam
Mir, Adil Shamim
Azimirad, Masoumeh
Balaii, Hedieh
Shahrokh, Shabnam
Zali, Mohammad Reza
author_facet Ghavami, Shaghayegh Baradaran
Yadegar, Abbas
Aghdaei, Hamid Asadzadeh
Sorrentino, Dario
Farmani, Maryam
Mir, Adil Shamim
Azimirad, Masoumeh
Balaii, Hedieh
Shahrokh, Shabnam
Zali, Mohammad Reza
author_sort Ghavami, Shaghayegh Baradaran
collection PubMed
description In inflammatory bowel diseases (IBD), the therapeutic benefit and mucosal healing from specific probiotics may relate to the modulation of dendritic cells (DCs). Herein, we assessed the immunomodulatory effects of four probiotic strains including Lactobacillus salivarius, Bifidobacterium bifidum, Bacillus coagulans and Bacillus subtilis natto on the expression of co-stimulatory molecules, cytokine production and gene expression of signal-transducing receptors in DCs from IBD patients. Human monocyte-derived DCs from IBD patients and healthy controls were exposed to four probiotic strains. The expression of co-stimulatory molecules was assessed and supernatants were analyzed for anti-inflammatory cytokines. The gene expression of toll-like receptors (TLRs), IL-12p40 and integrin αvβ8 were also analyzed. CD80 and CD86 were induced by most probiotic strains in ulcerative colitis (UC) patients whereas only B. bifidum induced CD80 and CD86 expression in Crohn’s disease (CD) patients. IL-10 and TGF-β production was increased in a dose-independent manner while TLR expression was decreased by all probiotic bacteria except B. bifidum in DCs from UC patients. TLR-4 and TLR-9 expression was significantly downregulated while integrin ß8 was significantly increased in the DCs from CD patients. IL-12p40 expression was only significantly downregulated in DCs from CD patients. Our findings point to the general beneficial effects of probiotics in DC immunomodulation and indicate that probiotic bacteria favorably modulate the expression of co-stimulatory molecules, proinflammatory cytokines and TLRs in DCs from IBD patients.
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spelling pubmed-75035522020-09-23 Immunomodulation and Generation of Tolerogenic Dendritic Cells by Probiotic Bacteria in Patients with Inflammatory Bowel Disease Ghavami, Shaghayegh Baradaran Yadegar, Abbas Aghdaei, Hamid Asadzadeh Sorrentino, Dario Farmani, Maryam Mir, Adil Shamim Azimirad, Masoumeh Balaii, Hedieh Shahrokh, Shabnam Zali, Mohammad Reza Int J Mol Sci Article In inflammatory bowel diseases (IBD), the therapeutic benefit and mucosal healing from specific probiotics may relate to the modulation of dendritic cells (DCs). Herein, we assessed the immunomodulatory effects of four probiotic strains including Lactobacillus salivarius, Bifidobacterium bifidum, Bacillus coagulans and Bacillus subtilis natto on the expression of co-stimulatory molecules, cytokine production and gene expression of signal-transducing receptors in DCs from IBD patients. Human monocyte-derived DCs from IBD patients and healthy controls were exposed to four probiotic strains. The expression of co-stimulatory molecules was assessed and supernatants were analyzed for anti-inflammatory cytokines. The gene expression of toll-like receptors (TLRs), IL-12p40 and integrin αvβ8 were also analyzed. CD80 and CD86 were induced by most probiotic strains in ulcerative colitis (UC) patients whereas only B. bifidum induced CD80 and CD86 expression in Crohn’s disease (CD) patients. IL-10 and TGF-β production was increased in a dose-independent manner while TLR expression was decreased by all probiotic bacteria except B. bifidum in DCs from UC patients. TLR-4 and TLR-9 expression was significantly downregulated while integrin ß8 was significantly increased in the DCs from CD patients. IL-12p40 expression was only significantly downregulated in DCs from CD patients. Our findings point to the general beneficial effects of probiotics in DC immunomodulation and indicate that probiotic bacteria favorably modulate the expression of co-stimulatory molecules, proinflammatory cytokines and TLRs in DCs from IBD patients. MDPI 2020-08-29 /pmc/articles/PMC7503552/ /pubmed/32872480 http://dx.doi.org/10.3390/ijms21176266 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ghavami, Shaghayegh Baradaran
Yadegar, Abbas
Aghdaei, Hamid Asadzadeh
Sorrentino, Dario
Farmani, Maryam
Mir, Adil Shamim
Azimirad, Masoumeh
Balaii, Hedieh
Shahrokh, Shabnam
Zali, Mohammad Reza
Immunomodulation and Generation of Tolerogenic Dendritic Cells by Probiotic Bacteria in Patients with Inflammatory Bowel Disease
title Immunomodulation and Generation of Tolerogenic Dendritic Cells by Probiotic Bacteria in Patients with Inflammatory Bowel Disease
title_full Immunomodulation and Generation of Tolerogenic Dendritic Cells by Probiotic Bacteria in Patients with Inflammatory Bowel Disease
title_fullStr Immunomodulation and Generation of Tolerogenic Dendritic Cells by Probiotic Bacteria in Patients with Inflammatory Bowel Disease
title_full_unstemmed Immunomodulation and Generation of Tolerogenic Dendritic Cells by Probiotic Bacteria in Patients with Inflammatory Bowel Disease
title_short Immunomodulation and Generation of Tolerogenic Dendritic Cells by Probiotic Bacteria in Patients with Inflammatory Bowel Disease
title_sort immunomodulation and generation of tolerogenic dendritic cells by probiotic bacteria in patients with inflammatory bowel disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503552/
https://www.ncbi.nlm.nih.gov/pubmed/32872480
http://dx.doi.org/10.3390/ijms21176266
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