Cargando…
Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells on Amine-Functionalized Titanium Using Humidified Ammonia Supplied Nonthermal Atmospheric Pressure Plasma
The surface molecular chemistry, such as amine functionality, of biomaterials plays a crucial role in the osteogenic activity of relevant cells and tissues during hard tissue regeneration. Here, we examined the possibilities of creating amine functionalities on the surface of titanium by using the n...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503675/ https://www.ncbi.nlm.nih.gov/pubmed/32846976 http://dx.doi.org/10.3390/ijms21176085 |
_version_ | 1783584449143767040 |
---|---|
author | Kwon, Jae-Sung Choi, Sung-Hwan Choi, Eun Ha Kim, Kwang-Mahn Chu, Paul K. |
author_facet | Kwon, Jae-Sung Choi, Sung-Hwan Choi, Eun Ha Kim, Kwang-Mahn Chu, Paul K. |
author_sort | Kwon, Jae-Sung |
collection | PubMed |
description | The surface molecular chemistry, such as amine functionality, of biomaterials plays a crucial role in the osteogenic activity of relevant cells and tissues during hard tissue regeneration. Here, we examined the possibilities of creating amine functionalities on the surface of titanium by using the nonthermal atmospheric pressure plasma jet (NTAPPJ) method with humidified ammonia, and the effects on human mesenchymal stem cell (hMSC) were investigated. Titanium samples were subjected to NTAPPJ treatments using nitrogen (N-P), air (A-P), or humidified ammonia (NA-P) as the plasma gas, while control (C-P) samples were not subjected to plasma treatment. After plasma exposure, all treatment groups showed increased hydrophilicity and had more attached cells than the C-P. Among the plasma-treated samples, the A-P and NA-P showed surface oxygen functionalities and exhibited greater cell proliferation than the C-P and N-P. The NA-P additionally showed surface amine-related functionalities and exhibited a higher level of alkaline phosphatase activity and osteocalcin expression than the other samples. The results can be explained by increases in fibronectin absorption and focal adhesion kinase gene expression on the NA-P samples. These findings suggest that NTAPPJ technology with humidified ammonia as a gas source has clinical potential for hard tissue generation. |
format | Online Article Text |
id | pubmed-7503675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75036752020-09-27 Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells on Amine-Functionalized Titanium Using Humidified Ammonia Supplied Nonthermal Atmospheric Pressure Plasma Kwon, Jae-Sung Choi, Sung-Hwan Choi, Eun Ha Kim, Kwang-Mahn Chu, Paul K. Int J Mol Sci Article The surface molecular chemistry, such as amine functionality, of biomaterials plays a crucial role in the osteogenic activity of relevant cells and tissues during hard tissue regeneration. Here, we examined the possibilities of creating amine functionalities on the surface of titanium by using the nonthermal atmospheric pressure plasma jet (NTAPPJ) method with humidified ammonia, and the effects on human mesenchymal stem cell (hMSC) were investigated. Titanium samples were subjected to NTAPPJ treatments using nitrogen (N-P), air (A-P), or humidified ammonia (NA-P) as the plasma gas, while control (C-P) samples were not subjected to plasma treatment. After plasma exposure, all treatment groups showed increased hydrophilicity and had more attached cells than the C-P. Among the plasma-treated samples, the A-P and NA-P showed surface oxygen functionalities and exhibited greater cell proliferation than the C-P and N-P. The NA-P additionally showed surface amine-related functionalities and exhibited a higher level of alkaline phosphatase activity and osteocalcin expression than the other samples. The results can be explained by increases in fibronectin absorption and focal adhesion kinase gene expression on the NA-P samples. These findings suggest that NTAPPJ technology with humidified ammonia as a gas source has clinical potential for hard tissue generation. MDPI 2020-08-24 /pmc/articles/PMC7503675/ /pubmed/32846976 http://dx.doi.org/10.3390/ijms21176085 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kwon, Jae-Sung Choi, Sung-Hwan Choi, Eun Ha Kim, Kwang-Mahn Chu, Paul K. Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells on Amine-Functionalized Titanium Using Humidified Ammonia Supplied Nonthermal Atmospheric Pressure Plasma |
title | Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells on Amine-Functionalized Titanium Using Humidified Ammonia Supplied Nonthermal Atmospheric Pressure Plasma |
title_full | Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells on Amine-Functionalized Titanium Using Humidified Ammonia Supplied Nonthermal Atmospheric Pressure Plasma |
title_fullStr | Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells on Amine-Functionalized Titanium Using Humidified Ammonia Supplied Nonthermal Atmospheric Pressure Plasma |
title_full_unstemmed | Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells on Amine-Functionalized Titanium Using Humidified Ammonia Supplied Nonthermal Atmospheric Pressure Plasma |
title_short | Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells on Amine-Functionalized Titanium Using Humidified Ammonia Supplied Nonthermal Atmospheric Pressure Plasma |
title_sort | enhanced osteogenic differentiation of human mesenchymal stem cells on amine-functionalized titanium using humidified ammonia supplied nonthermal atmospheric pressure plasma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503675/ https://www.ncbi.nlm.nih.gov/pubmed/32846976 http://dx.doi.org/10.3390/ijms21176085 |
work_keys_str_mv | AT kwonjaesung enhancedosteogenicdifferentiationofhumanmesenchymalstemcellsonaminefunctionalizedtitaniumusinghumidifiedammoniasuppliednonthermalatmosphericpressureplasma AT choisunghwan enhancedosteogenicdifferentiationofhumanmesenchymalstemcellsonaminefunctionalizedtitaniumusinghumidifiedammoniasuppliednonthermalatmosphericpressureplasma AT choieunha enhancedosteogenicdifferentiationofhumanmesenchymalstemcellsonaminefunctionalizedtitaniumusinghumidifiedammoniasuppliednonthermalatmosphericpressureplasma AT kimkwangmahn enhancedosteogenicdifferentiationofhumanmesenchymalstemcellsonaminefunctionalizedtitaniumusinghumidifiedammoniasuppliednonthermalatmosphericpressureplasma AT chupaulk enhancedosteogenicdifferentiationofhumanmesenchymalstemcellsonaminefunctionalizedtitaniumusinghumidifiedammoniasuppliednonthermalatmosphericpressureplasma |