Cargando…
Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors
Manufacturing of mesenchymal stromal cell (MSC)-based therapies for regenerative medicine requires the use of suitable supply of growth factors that enhance proliferation, cell stability and potency during cell expansion. Human blood derivatives such as human platelet lysate (hPL) have emerged as a...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503902/ https://www.ncbi.nlm.nih.gov/pubmed/32877987 http://dx.doi.org/10.3390/ijms21176284 |
_version_ | 1783584499767967744 |
---|---|
author | Cañas-Arboleda, Mariana Beltrán, Karl Medina, Carlos Camacho, Bernardo Salguero, Gustavo |
author_facet | Cañas-Arboleda, Mariana Beltrán, Karl Medina, Carlos Camacho, Bernardo Salguero, Gustavo |
author_sort | Cañas-Arboleda, Mariana |
collection | PubMed |
description | Manufacturing of mesenchymal stromal cell (MSC)-based therapies for regenerative medicine requires the use of suitable supply of growth factors that enhance proliferation, cell stability and potency during cell expansion. Human blood derivatives such as human platelet lysate (hPL) have emerged as a feasible alternative for cell growth supplement. Nevertheless, composition and functional characterization of hPL in the context of cell manufacturing is still under investigation, particularly regarding the content and function of pro-survival and pro-regenerative factors. We performed comparative analyses of hPL, human serum (hS) and fetal bovine serum (FBS) stability and potency to support Wharton’s jelly (WJ) MSC production. We demonstrated that hPL displayed low inter-batch variation and unique secretome profile that was not present in hS and FBS. Importantly, hPL-derived factors including PDGF family, EGF, TGF-alpha, angiogenin and RANTES were actively taken up by WJ-MSC to support efficient expansion. Moreover, hPL but not hS or FBS induced secretion of osteoprotegerin, HGF, IL-6 and GRO-alpha by WJ-MSC during the expansion phase. Thus, hPL is a suitable source of factors supporting viability, stability and potency of WJ-MSC and therefore constitutes an essential raw material that in combination with WJ-MSC introduces a great opportunity for the generation of potent regenerative medicine products. |
format | Online Article Text |
id | pubmed-7503902 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75039022020-09-27 Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors Cañas-Arboleda, Mariana Beltrán, Karl Medina, Carlos Camacho, Bernardo Salguero, Gustavo Int J Mol Sci Article Manufacturing of mesenchymal stromal cell (MSC)-based therapies for regenerative medicine requires the use of suitable supply of growth factors that enhance proliferation, cell stability and potency during cell expansion. Human blood derivatives such as human platelet lysate (hPL) have emerged as a feasible alternative for cell growth supplement. Nevertheless, composition and functional characterization of hPL in the context of cell manufacturing is still under investigation, particularly regarding the content and function of pro-survival and pro-regenerative factors. We performed comparative analyses of hPL, human serum (hS) and fetal bovine serum (FBS) stability and potency to support Wharton’s jelly (WJ) MSC production. We demonstrated that hPL displayed low inter-batch variation and unique secretome profile that was not present in hS and FBS. Importantly, hPL-derived factors including PDGF family, EGF, TGF-alpha, angiogenin and RANTES were actively taken up by WJ-MSC to support efficient expansion. Moreover, hPL but not hS or FBS induced secretion of osteoprotegerin, HGF, IL-6 and GRO-alpha by WJ-MSC during the expansion phase. Thus, hPL is a suitable source of factors supporting viability, stability and potency of WJ-MSC and therefore constitutes an essential raw material that in combination with WJ-MSC introduces a great opportunity for the generation of potent regenerative medicine products. MDPI 2020-08-31 /pmc/articles/PMC7503902/ /pubmed/32877987 http://dx.doi.org/10.3390/ijms21176284 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cañas-Arboleda, Mariana Beltrán, Karl Medina, Carlos Camacho, Bernardo Salguero, Gustavo Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors |
title | Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors |
title_full | Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors |
title_fullStr | Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors |
title_full_unstemmed | Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors |
title_short | Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors |
title_sort | human platelet lysate supports efficient expansion and stability of wharton’s jelly mesenchymal stromal cells via active uptake and release of soluble regenerative factors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503902/ https://www.ncbi.nlm.nih.gov/pubmed/32877987 http://dx.doi.org/10.3390/ijms21176284 |
work_keys_str_mv | AT canasarboledamariana humanplateletlysatesupportsefficientexpansionandstabilityofwhartonsjellymesenchymalstromalcellsviaactiveuptakeandreleaseofsolubleregenerativefactors AT beltrankarl humanplateletlysatesupportsefficientexpansionandstabilityofwhartonsjellymesenchymalstromalcellsviaactiveuptakeandreleaseofsolubleregenerativefactors AT medinacarlos humanplateletlysatesupportsefficientexpansionandstabilityofwhartonsjellymesenchymalstromalcellsviaactiveuptakeandreleaseofsolubleregenerativefactors AT camachobernardo humanplateletlysatesupportsefficientexpansionandstabilityofwhartonsjellymesenchymalstromalcellsviaactiveuptakeandreleaseofsolubleregenerativefactors AT salguerogustavo humanplateletlysatesupportsefficientexpansionandstabilityofwhartonsjellymesenchymalstromalcellsviaactiveuptakeandreleaseofsolubleregenerativefactors |