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In Vitro Studies on Antioxidant and Anti-Parasitic Activities of Compounds Isolated from Rauvolfia caffra Sond

As part of an ongoing study of natural products from local medicinal plants, the methanol extract of stem bark of Rauvolfia caffra Sond was investigated for biological activity. Column chromatography and preparative thin-layer chromatography were used to isolate lupeol (1), raucaffricine (2), N-meth...

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Autores principales: Tlhapi, Dorcas B., Ramaite, Isaiah D. I., Anokwuru, Chinedu P., van Ree, Teunis, Hoppe, Heinrich C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503976/
https://www.ncbi.nlm.nih.gov/pubmed/32825216
http://dx.doi.org/10.3390/molecules25173781
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author Tlhapi, Dorcas B.
Ramaite, Isaiah D. I.
Anokwuru, Chinedu P.
van Ree, Teunis
Hoppe, Heinrich C.
author_facet Tlhapi, Dorcas B.
Ramaite, Isaiah D. I.
Anokwuru, Chinedu P.
van Ree, Teunis
Hoppe, Heinrich C.
author_sort Tlhapi, Dorcas B.
collection PubMed
description As part of an ongoing study of natural products from local medicinal plants, the methanol extract of stem bark of Rauvolfia caffra Sond was investigated for biological activity. Column chromatography and preparative thin-layer chromatography were used to isolate lupeol (1), raucaffricine (2), N-methylsarpagine (3), and spegatrine (4). The crude extract, fractions and isolated compounds were tested for anti-oxidant, antitrypanosomal and anti-proliferation activities. Two fractions displayed high DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity and reducing power with IC(50) (The half maximal inhibitory concentration) and IC(0.5) values of 0.022 ± 0.003 mg/mL and 0.036 ± 0.007 mg/mL, and 0.518 ± 0.044 mg/mL and 1.076 ± 0.136 mg/mL, respectively. Spegatrine (4) was identified as the main antioxidant compound in R. caffra with IC(50) and IC(0.5) values of 0.119 ± 0.067 mg/mL and 0.712 ± 0 mg/mL, respectively. One fraction displayed high antitrypanosomal activity with an IC(50) value of 18.50 μg/mL. However, the major constituent of this fraction, raucaffricine (2), was not active. The crude extract, fractions and pure compounds did not display any cytotoxic effect at a concentration of 50 μg/mL against HeLa cells. This study shows directions for further in vitro studies on the antioxidant and antitrypanosomal activities of Rauvolfia caffra Sond.
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spelling pubmed-75039762020-09-27 In Vitro Studies on Antioxidant and Anti-Parasitic Activities of Compounds Isolated from Rauvolfia caffra Sond Tlhapi, Dorcas B. Ramaite, Isaiah D. I. Anokwuru, Chinedu P. van Ree, Teunis Hoppe, Heinrich C. Molecules Communication As part of an ongoing study of natural products from local medicinal plants, the methanol extract of stem bark of Rauvolfia caffra Sond was investigated for biological activity. Column chromatography and preparative thin-layer chromatography were used to isolate lupeol (1), raucaffricine (2), N-methylsarpagine (3), and spegatrine (4). The crude extract, fractions and isolated compounds were tested for anti-oxidant, antitrypanosomal and anti-proliferation activities. Two fractions displayed high DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity and reducing power with IC(50) (The half maximal inhibitory concentration) and IC(0.5) values of 0.022 ± 0.003 mg/mL and 0.036 ± 0.007 mg/mL, and 0.518 ± 0.044 mg/mL and 1.076 ± 0.136 mg/mL, respectively. Spegatrine (4) was identified as the main antioxidant compound in R. caffra with IC(50) and IC(0.5) values of 0.119 ± 0.067 mg/mL and 0.712 ± 0 mg/mL, respectively. One fraction displayed high antitrypanosomal activity with an IC(50) value of 18.50 μg/mL. However, the major constituent of this fraction, raucaffricine (2), was not active. The crude extract, fractions and pure compounds did not display any cytotoxic effect at a concentration of 50 μg/mL against HeLa cells. This study shows directions for further in vitro studies on the antioxidant and antitrypanosomal activities of Rauvolfia caffra Sond. MDPI 2020-08-20 /pmc/articles/PMC7503976/ /pubmed/32825216 http://dx.doi.org/10.3390/molecules25173781 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Tlhapi, Dorcas B.
Ramaite, Isaiah D. I.
Anokwuru, Chinedu P.
van Ree, Teunis
Hoppe, Heinrich C.
In Vitro Studies on Antioxidant and Anti-Parasitic Activities of Compounds Isolated from Rauvolfia caffra Sond
title In Vitro Studies on Antioxidant and Anti-Parasitic Activities of Compounds Isolated from Rauvolfia caffra Sond
title_full In Vitro Studies on Antioxidant and Anti-Parasitic Activities of Compounds Isolated from Rauvolfia caffra Sond
title_fullStr In Vitro Studies on Antioxidant and Anti-Parasitic Activities of Compounds Isolated from Rauvolfia caffra Sond
title_full_unstemmed In Vitro Studies on Antioxidant and Anti-Parasitic Activities of Compounds Isolated from Rauvolfia caffra Sond
title_short In Vitro Studies on Antioxidant and Anti-Parasitic Activities of Compounds Isolated from Rauvolfia caffra Sond
title_sort in vitro studies on antioxidant and anti-parasitic activities of compounds isolated from rauvolfia caffra sond
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503976/
https://www.ncbi.nlm.nih.gov/pubmed/32825216
http://dx.doi.org/10.3390/molecules25173781
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