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Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA(LPS)) Occurs in Rough Strains of Escherichia coli R1, R2, and R4
Enterobacterial common antigen (ECA) is a conserved surface antigen characteristic for Enterobacteriaceae. It is consisting of trisaccharide repeating unit, →3)-α-d-Fucp4NAc-(1→4)-β-d-ManpNAcA-(1→4)-α-d-GlcpNAc-(1→, where prevailing forms include ECA linked to phosphatidylglycerol (ECA(PG)) and cycl...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504096/ https://www.ncbi.nlm.nih.gov/pubmed/32839412 http://dx.doi.org/10.3390/ijms21176038 |
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author | Maciejewska, Anna Kaszowska, Marta Jachymek, Wojciech Lugowski, Czeslaw Lukasiewicz, Jolanta |
author_facet | Maciejewska, Anna Kaszowska, Marta Jachymek, Wojciech Lugowski, Czeslaw Lukasiewicz, Jolanta |
author_sort | Maciejewska, Anna |
collection | PubMed |
description | Enterobacterial common antigen (ECA) is a conserved surface antigen characteristic for Enterobacteriaceae. It is consisting of trisaccharide repeating unit, →3)-α-d-Fucp4NAc-(1→4)-β-d-ManpNAcA-(1→4)-α-d-GlcpNAc-(1→, where prevailing forms include ECA linked to phosphatidylglycerol (ECA(PG)) and cyclic ECA (ECA(CYC)). Lipopolysaccharide (LPS)-associated form (ECA(LPS)) has been proved to date only for rough Shigella sonnei phase II. Depending on the structure organization, ECA constitutes surface antigen (ECA(PG) and ECA(LPS)) or maintains the outer membrane permeability barrier (ECA(CYC)). The existence of LPS was hypothesized in the 1960–80s on the basis of serological observations. Only a few Escherichia coli strains (i.e., R1, R2, R3, R4, and K-12) have led to the generation of anti-ECA antibodies upon immunization, excluding ECA(PG) as an immunogen and conjecturing ECA(LPS) as the only immunogenic form. Here, we presented a structural survey of ECA(LPS) in E. coli R1, R2, R3, and R4 to correlate previous serological observations with the presence of ECA(LPS). The low yields of ECA(LPS) were identified in the R1, R2, and R4 strains, where ECA occupied outer core residues of LPS that used to be substituted by O-specific polysaccharide in the case of smooth LPS. Previously published observations and hypotheses regarding the immunogenicity and biosynthesis of ECA(LPS) were discussed and correlated with presented herein structural data. |
format | Online Article Text |
id | pubmed-7504096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75040962020-09-24 Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA(LPS)) Occurs in Rough Strains of Escherichia coli R1, R2, and R4 Maciejewska, Anna Kaszowska, Marta Jachymek, Wojciech Lugowski, Czeslaw Lukasiewicz, Jolanta Int J Mol Sci Article Enterobacterial common antigen (ECA) is a conserved surface antigen characteristic for Enterobacteriaceae. It is consisting of trisaccharide repeating unit, →3)-α-d-Fucp4NAc-(1→4)-β-d-ManpNAcA-(1→4)-α-d-GlcpNAc-(1→, where prevailing forms include ECA linked to phosphatidylglycerol (ECA(PG)) and cyclic ECA (ECA(CYC)). Lipopolysaccharide (LPS)-associated form (ECA(LPS)) has been proved to date only for rough Shigella sonnei phase II. Depending on the structure organization, ECA constitutes surface antigen (ECA(PG) and ECA(LPS)) or maintains the outer membrane permeability barrier (ECA(CYC)). The existence of LPS was hypothesized in the 1960–80s on the basis of serological observations. Only a few Escherichia coli strains (i.e., R1, R2, R3, R4, and K-12) have led to the generation of anti-ECA antibodies upon immunization, excluding ECA(PG) as an immunogen and conjecturing ECA(LPS) as the only immunogenic form. Here, we presented a structural survey of ECA(LPS) in E. coli R1, R2, R3, and R4 to correlate previous serological observations with the presence of ECA(LPS). The low yields of ECA(LPS) were identified in the R1, R2, and R4 strains, where ECA occupied outer core residues of LPS that used to be substituted by O-specific polysaccharide in the case of smooth LPS. Previously published observations and hypotheses regarding the immunogenicity and biosynthesis of ECA(LPS) were discussed and correlated with presented herein structural data. MDPI 2020-08-21 /pmc/articles/PMC7504096/ /pubmed/32839412 http://dx.doi.org/10.3390/ijms21176038 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Maciejewska, Anna Kaszowska, Marta Jachymek, Wojciech Lugowski, Czeslaw Lukasiewicz, Jolanta Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA(LPS)) Occurs in Rough Strains of Escherichia coli R1, R2, and R4 |
title | Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA(LPS)) Occurs in Rough Strains of Escherichia coli R1, R2, and R4 |
title_full | Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA(LPS)) Occurs in Rough Strains of Escherichia coli R1, R2, and R4 |
title_fullStr | Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA(LPS)) Occurs in Rough Strains of Escherichia coli R1, R2, and R4 |
title_full_unstemmed | Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA(LPS)) Occurs in Rough Strains of Escherichia coli R1, R2, and R4 |
title_short | Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA(LPS)) Occurs in Rough Strains of Escherichia coli R1, R2, and R4 |
title_sort | lipopolysaccharide-linked enterobacterial common antigen (eca(lps)) occurs in rough strains of escherichia coli r1, r2, and r4 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504096/ https://www.ncbi.nlm.nih.gov/pubmed/32839412 http://dx.doi.org/10.3390/ijms21176038 |
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