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Anti-c-fms Antibody Prevents Osteoclast Formation and Bone Resorption in Co-Culture of Osteoblasts and Osteoclast Precursors In Vitro and in Ovariectomized Mice

Osteoporosis morphology is characterized by bone resorption and decreases in micro-architecture parameters. Anti-osteoporosis therapy targets osteoclasts because bone resorption is a unique function of osteoclasts. Anti-c-fms antibodies against the receptor for macrophage colony-stimulating factor (...

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Autores principales: Nara, Yasuhiko, Kitaura, Hideki, Ogawa, Saika, Shen, Wei-Ren, Qi, Jiawei, Ohori, Fumitoshi, Noguchi, Takahiro, Marahleh, Aseel, Pramusita, Adya, Kinjo, Ria, Mizoguchi, Itaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504124/
https://www.ncbi.nlm.nih.gov/pubmed/32854340
http://dx.doi.org/10.3390/ijms21176120
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author Nara, Yasuhiko
Kitaura, Hideki
Ogawa, Saika
Shen, Wei-Ren
Qi, Jiawei
Ohori, Fumitoshi
Noguchi, Takahiro
Marahleh, Aseel
Pramusita, Adya
Kinjo, Ria
Mizoguchi, Itaru
author_facet Nara, Yasuhiko
Kitaura, Hideki
Ogawa, Saika
Shen, Wei-Ren
Qi, Jiawei
Ohori, Fumitoshi
Noguchi, Takahiro
Marahleh, Aseel
Pramusita, Adya
Kinjo, Ria
Mizoguchi, Itaru
author_sort Nara, Yasuhiko
collection PubMed
description Osteoporosis morphology is characterized by bone resorption and decreases in micro-architecture parameters. Anti-osteoporosis therapy targets osteoclasts because bone resorption is a unique function of osteoclasts. Anti-c-fms antibodies against the receptor for macrophage colony-stimulating factor (M-CSF) inhibit osteoclast formation and bone resorption in vitro and in vivo. However, the effect of anti-c-fms antibodies on bone resorption in ovariectomized (OVX) mice is unknown. In this study, we evaluated the effect of anti-c-fms antibodies on osteoclast formation and bone resorption in osteoblast–osteoclast precursor co-culture in vitro and in OVX mice. Osteoblast and osteoclast precursor co-cultures treated with anti-c-fms antibodies showed significantly inhibited osteoclast formation, while cultures without anti-c-fms antibody treatment showed osteoclast formation. However, anti-c-fms antibodies did not change the receptor activator of nuclear factor kappa-B ligand (RANKL) or osteoprotegrin (OPG) expression during osteoblast and osteoclast differentiation in vitro. These results indicate that anti-c-fms antibodies directly affected osteoclast formation from osteoclast precursors in co-culture. OVX mice were treated with intraperitoneal injections of anti-c-fms antibody. The trabecular bone structure of the femur was assessed by micro-computer tomography. The anti-c-fms antibody inhibited osteoclast formation and bone loss compared with PBS-treated OVX mice. These results indicate potential for the therapeutic application of anti-c-fms antibodies for postmenopausal osteoporosis.
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spelling pubmed-75041242020-09-24 Anti-c-fms Antibody Prevents Osteoclast Formation and Bone Resorption in Co-Culture of Osteoblasts and Osteoclast Precursors In Vitro and in Ovariectomized Mice Nara, Yasuhiko Kitaura, Hideki Ogawa, Saika Shen, Wei-Ren Qi, Jiawei Ohori, Fumitoshi Noguchi, Takahiro Marahleh, Aseel Pramusita, Adya Kinjo, Ria Mizoguchi, Itaru Int J Mol Sci Article Osteoporosis morphology is characterized by bone resorption and decreases in micro-architecture parameters. Anti-osteoporosis therapy targets osteoclasts because bone resorption is a unique function of osteoclasts. Anti-c-fms antibodies against the receptor for macrophage colony-stimulating factor (M-CSF) inhibit osteoclast formation and bone resorption in vitro and in vivo. However, the effect of anti-c-fms antibodies on bone resorption in ovariectomized (OVX) mice is unknown. In this study, we evaluated the effect of anti-c-fms antibodies on osteoclast formation and bone resorption in osteoblast–osteoclast precursor co-culture in vitro and in OVX mice. Osteoblast and osteoclast precursor co-cultures treated with anti-c-fms antibodies showed significantly inhibited osteoclast formation, while cultures without anti-c-fms antibody treatment showed osteoclast formation. However, anti-c-fms antibodies did not change the receptor activator of nuclear factor kappa-B ligand (RANKL) or osteoprotegrin (OPG) expression during osteoblast and osteoclast differentiation in vitro. These results indicate that anti-c-fms antibodies directly affected osteoclast formation from osteoclast precursors in co-culture. OVX mice were treated with intraperitoneal injections of anti-c-fms antibody. The trabecular bone structure of the femur was assessed by micro-computer tomography. The anti-c-fms antibody inhibited osteoclast formation and bone loss compared with PBS-treated OVX mice. These results indicate potential for the therapeutic application of anti-c-fms antibodies for postmenopausal osteoporosis. MDPI 2020-08-25 /pmc/articles/PMC7504124/ /pubmed/32854340 http://dx.doi.org/10.3390/ijms21176120 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nara, Yasuhiko
Kitaura, Hideki
Ogawa, Saika
Shen, Wei-Ren
Qi, Jiawei
Ohori, Fumitoshi
Noguchi, Takahiro
Marahleh, Aseel
Pramusita, Adya
Kinjo, Ria
Mizoguchi, Itaru
Anti-c-fms Antibody Prevents Osteoclast Formation and Bone Resorption in Co-Culture of Osteoblasts and Osteoclast Precursors In Vitro and in Ovariectomized Mice
title Anti-c-fms Antibody Prevents Osteoclast Formation and Bone Resorption in Co-Culture of Osteoblasts and Osteoclast Precursors In Vitro and in Ovariectomized Mice
title_full Anti-c-fms Antibody Prevents Osteoclast Formation and Bone Resorption in Co-Culture of Osteoblasts and Osteoclast Precursors In Vitro and in Ovariectomized Mice
title_fullStr Anti-c-fms Antibody Prevents Osteoclast Formation and Bone Resorption in Co-Culture of Osteoblasts and Osteoclast Precursors In Vitro and in Ovariectomized Mice
title_full_unstemmed Anti-c-fms Antibody Prevents Osteoclast Formation and Bone Resorption in Co-Culture of Osteoblasts and Osteoclast Precursors In Vitro and in Ovariectomized Mice
title_short Anti-c-fms Antibody Prevents Osteoclast Formation and Bone Resorption in Co-Culture of Osteoblasts and Osteoclast Precursors In Vitro and in Ovariectomized Mice
title_sort anti-c-fms antibody prevents osteoclast formation and bone resorption in co-culture of osteoblasts and osteoclast precursors in vitro and in ovariectomized mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504124/
https://www.ncbi.nlm.nih.gov/pubmed/32854340
http://dx.doi.org/10.3390/ijms21176120
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