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Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro
Human dental pulp harbours unique stem cell population exhibiting mesenchymal stem/stromal cell (MSC) characteristics. This study aimed to analyse the differentiation potential and other essential functional and morphological features of dental pulp stem cells (DPSCs) in comparison with Wharton’s je...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504399/ https://www.ncbi.nlm.nih.gov/pubmed/32859105 http://dx.doi.org/10.3390/ijms21176172 |
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author | Labedz-Maslowska, Anna Bryniarska, Natalia Kubiak, Andrzej Kaczmarzyk, Tomasz Sekula-Stryjewska, Malgorzata Noga, Sylwia Boruczkowski, Dariusz Madeja, Zbigniew Zuba-Surma, Ewa |
author_facet | Labedz-Maslowska, Anna Bryniarska, Natalia Kubiak, Andrzej Kaczmarzyk, Tomasz Sekula-Stryjewska, Malgorzata Noga, Sylwia Boruczkowski, Dariusz Madeja, Zbigniew Zuba-Surma, Ewa |
author_sort | Labedz-Maslowska, Anna |
collection | PubMed |
description | Human dental pulp harbours unique stem cell population exhibiting mesenchymal stem/stromal cell (MSC) characteristics. This study aimed to analyse the differentiation potential and other essential functional and morphological features of dental pulp stem cells (DPSCs) in comparison with Wharton’s jelly-derived MSCs from the umbilical cord (UC-MSCs), and to evaluate the osteogenic differentiation of DPSCs in 3D culture with a hypoxic microenvironment resembling the stem cell niche. Human DPSCs as well as UC-MSCs were isolated from primary human tissues and were subjected to a series of experiments. We established a multiantigenic profile of DPSCs with CD45(−)/CD14(−)/CD34(−)/CD29(+)/CD44(+)/CD73(+)/CD90(+)/CD105(+)/Stro-1(+)/HLA-DR(−) (using flow cytometry) and confirmed their tri-lineage osteogenic, chondrogenic, and adipogenic differentiation potential (using qRT-PCR and histochemical staining) in comparison with the UC-MSCs. The results also demonstrated the potency of DPSCs to differentiate into osteoblasts in vitro. Moreover, we showed that the DPSCs exhibit limited cardiomyogenic and endothelial differentiation potential. Decreased proliferation and metabolic activity as well as increased osteogenic differentiation of DPSCs in vitro, attributed to 3D cell encapsulation and low oxygen concentration, were also observed. DPSCs exhibiting elevated osteogenic potential may serve as potential candidates for a cell-based product for advanced therapy, particularly for bone repair. Novel tissue engineering approaches combining DPSCs, 3D biomaterial scaffolds, and other stimulating chemical factors may represent innovative strategies for pro-regenerative therapies. |
format | Online Article Text |
id | pubmed-7504399 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75043992020-09-24 Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro Labedz-Maslowska, Anna Bryniarska, Natalia Kubiak, Andrzej Kaczmarzyk, Tomasz Sekula-Stryjewska, Malgorzata Noga, Sylwia Boruczkowski, Dariusz Madeja, Zbigniew Zuba-Surma, Ewa Int J Mol Sci Article Human dental pulp harbours unique stem cell population exhibiting mesenchymal stem/stromal cell (MSC) characteristics. This study aimed to analyse the differentiation potential and other essential functional and morphological features of dental pulp stem cells (DPSCs) in comparison with Wharton’s jelly-derived MSCs from the umbilical cord (UC-MSCs), and to evaluate the osteogenic differentiation of DPSCs in 3D culture with a hypoxic microenvironment resembling the stem cell niche. Human DPSCs as well as UC-MSCs were isolated from primary human tissues and were subjected to a series of experiments. We established a multiantigenic profile of DPSCs with CD45(−)/CD14(−)/CD34(−)/CD29(+)/CD44(+)/CD73(+)/CD90(+)/CD105(+)/Stro-1(+)/HLA-DR(−) (using flow cytometry) and confirmed their tri-lineage osteogenic, chondrogenic, and adipogenic differentiation potential (using qRT-PCR and histochemical staining) in comparison with the UC-MSCs. The results also demonstrated the potency of DPSCs to differentiate into osteoblasts in vitro. Moreover, we showed that the DPSCs exhibit limited cardiomyogenic and endothelial differentiation potential. Decreased proliferation and metabolic activity as well as increased osteogenic differentiation of DPSCs in vitro, attributed to 3D cell encapsulation and low oxygen concentration, were also observed. DPSCs exhibiting elevated osteogenic potential may serve as potential candidates for a cell-based product for advanced therapy, particularly for bone repair. Novel tissue engineering approaches combining DPSCs, 3D biomaterial scaffolds, and other stimulating chemical factors may represent innovative strategies for pro-regenerative therapies. MDPI 2020-08-26 /pmc/articles/PMC7504399/ /pubmed/32859105 http://dx.doi.org/10.3390/ijms21176172 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Labedz-Maslowska, Anna Bryniarska, Natalia Kubiak, Andrzej Kaczmarzyk, Tomasz Sekula-Stryjewska, Malgorzata Noga, Sylwia Boruczkowski, Dariusz Madeja, Zbigniew Zuba-Surma, Ewa Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro |
title | Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro |
title_full | Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro |
title_fullStr | Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro |
title_full_unstemmed | Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro |
title_short | Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro |
title_sort | multilineage differentiation potential of human dental pulp stem cells—impact of 3d and hypoxic environment on osteogenesis in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504399/ https://www.ncbi.nlm.nih.gov/pubmed/32859105 http://dx.doi.org/10.3390/ijms21176172 |
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