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Characterizing the Relapse Potential in Different Luminal Subtypes of Breast Cancers with Functional Proteomics

Poor prognosis due to the high relapse and metastasis rates of breast cancer has been particularly linked to the luminal B subtype. The current study utilized MCF-7 and ZR-75-1 to investigate various luminal subtypes of breast cancers that have discrepant expressions in the estrogen receptor (ER) an...

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Autores principales: Lin, Tung-Yi, Wang, Pei-Wen, Huang, Chun-Hsun, Yang, Pei-Ming, Pan, Tai-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504407/
https://www.ncbi.nlm.nih.gov/pubmed/32846884
http://dx.doi.org/10.3390/ijms21176077
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author Lin, Tung-Yi
Wang, Pei-Wen
Huang, Chun-Hsun
Yang, Pei-Ming
Pan, Tai-Long
author_facet Lin, Tung-Yi
Wang, Pei-Wen
Huang, Chun-Hsun
Yang, Pei-Ming
Pan, Tai-Long
author_sort Lin, Tung-Yi
collection PubMed
description Poor prognosis due to the high relapse and metastasis rates of breast cancer has been particularly linked to the luminal B subtype. The current study utilized MCF-7 and ZR-75-1 to investigate various luminal subtypes of breast cancers that have discrepant expressions in the estrogen receptor (ER) and human epidermal growth factor receptor 2 (HER2). Understanding of the differential protein profiles and the associated pathways could help alleviate the malignance and promote the long-term survival rate of breast cancer patients. Functional proteome tools were applied to comprehensively delineate the global protein alterations that reflect the varieties of biological features between the two subtypes. In this study, a total of 11 proteins with significant and meaningful changes were identified. These protein targets including PRX2, CK19, nucleophosmin and cathepsin D were mostly involved in cell differentiation or proliferation. Particularly, cathepsin D was highly expressed in the luminal B subtype. Moreover, the level of cathepsin-D was also upregulated in the clinical metastatic tissues. Accordingly, the RNA interference-mediated silencing of cathepsin D stimulated ER expression but suppressed the level of HER2. The knockdown of cathepsin D enhanced the level of ZO-1 and a remarkable decrease in N-cadherin was also detected. Again, the matrix metalloproteinases (MMP) activity was impaired under the cathepsin D abolishment. Collectively, this study represented a modality to explore novel relationships in a proteome complex and highlighted the functional roles of cathepsin D in treatment options for different subtypes of breast cancer.
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spelling pubmed-75044072020-09-24 Characterizing the Relapse Potential in Different Luminal Subtypes of Breast Cancers with Functional Proteomics Lin, Tung-Yi Wang, Pei-Wen Huang, Chun-Hsun Yang, Pei-Ming Pan, Tai-Long Int J Mol Sci Article Poor prognosis due to the high relapse and metastasis rates of breast cancer has been particularly linked to the luminal B subtype. The current study utilized MCF-7 and ZR-75-1 to investigate various luminal subtypes of breast cancers that have discrepant expressions in the estrogen receptor (ER) and human epidermal growth factor receptor 2 (HER2). Understanding of the differential protein profiles and the associated pathways could help alleviate the malignance and promote the long-term survival rate of breast cancer patients. Functional proteome tools were applied to comprehensively delineate the global protein alterations that reflect the varieties of biological features between the two subtypes. In this study, a total of 11 proteins with significant and meaningful changes were identified. These protein targets including PRX2, CK19, nucleophosmin and cathepsin D were mostly involved in cell differentiation or proliferation. Particularly, cathepsin D was highly expressed in the luminal B subtype. Moreover, the level of cathepsin-D was also upregulated in the clinical metastatic tissues. Accordingly, the RNA interference-mediated silencing of cathepsin D stimulated ER expression but suppressed the level of HER2. The knockdown of cathepsin D enhanced the level of ZO-1 and a remarkable decrease in N-cadherin was also detected. Again, the matrix metalloproteinases (MMP) activity was impaired under the cathepsin D abolishment. Collectively, this study represented a modality to explore novel relationships in a proteome complex and highlighted the functional roles of cathepsin D in treatment options for different subtypes of breast cancer. MDPI 2020-08-24 /pmc/articles/PMC7504407/ /pubmed/32846884 http://dx.doi.org/10.3390/ijms21176077 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Tung-Yi
Wang, Pei-Wen
Huang, Chun-Hsun
Yang, Pei-Ming
Pan, Tai-Long
Characterizing the Relapse Potential in Different Luminal Subtypes of Breast Cancers with Functional Proteomics
title Characterizing the Relapse Potential in Different Luminal Subtypes of Breast Cancers with Functional Proteomics
title_full Characterizing the Relapse Potential in Different Luminal Subtypes of Breast Cancers with Functional Proteomics
title_fullStr Characterizing the Relapse Potential in Different Luminal Subtypes of Breast Cancers with Functional Proteomics
title_full_unstemmed Characterizing the Relapse Potential in Different Luminal Subtypes of Breast Cancers with Functional Proteomics
title_short Characterizing the Relapse Potential in Different Luminal Subtypes of Breast Cancers with Functional Proteomics
title_sort characterizing the relapse potential in different luminal subtypes of breast cancers with functional proteomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504407/
https://www.ncbi.nlm.nih.gov/pubmed/32846884
http://dx.doi.org/10.3390/ijms21176077
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