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Gestational Diabetes Type 2: Variation in High-Density Lipoproteins Composition and Function

Aims: Class A2 gestational diabetes mellitus (GDMA2) has short- and long-term effects on the mother and child. These may include abnormalities of placentation, damage to endothelial cells and cardiovascular disease. This research investigated the function and composition of high-density lipoproteins...

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Autores principales: Pasternak, Yael, Biron-Shental, Tal, Ohana, Meital, Einbinder, Yael, Arbib, Nissim, Benchetrit, Sydney, Zitman-Gal, Tali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504441/
https://www.ncbi.nlm.nih.gov/pubmed/32872663
http://dx.doi.org/10.3390/ijms21176281
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author Pasternak, Yael
Biron-Shental, Tal
Ohana, Meital
Einbinder, Yael
Arbib, Nissim
Benchetrit, Sydney
Zitman-Gal, Tali
author_facet Pasternak, Yael
Biron-Shental, Tal
Ohana, Meital
Einbinder, Yael
Arbib, Nissim
Benchetrit, Sydney
Zitman-Gal, Tali
author_sort Pasternak, Yael
collection PubMed
description Aims: Class A2 gestational diabetes mellitus (GDMA2) has short- and long-term effects on the mother and child. These may include abnormalities of placentation, damage to endothelial cells and cardiovascular disease. This research investigated the function and composition of high-density lipoproteins (HDL) among women with GDMA2 and their fetuses. Methods: Thirty pregnant women were recruited during admission for delivery. The function and expression of HDL, paraoxonase1 (PON1) and apolipoprotein A1 (APOA1) in the blood samples and the placental tissue were evaluated. The effect of HDL on migration of endothelial cells was measured in vitro. Results: Compared to normal pregnancy (NP), APOA1 in the maternal plasma of women with GDMA2 was decreased. More APOA1 and PON1 were released from HDL of women with GDMA2, compared to NP. Placental APOA1 and PON1 were decreased in GDMA2. For endothelial cells stimulated with TNFα, HDL cell migration was decreased when cells were evaluated with NP-HDL, as compared to GDMA2-HDL. Conclusions: GDMA2 affects the composition and function of HDL in plasma. Changes in HDL commonly seen in GDMA2 were observed in maternal and placental samples, but not in cord samples. These results might indicate a placental role in protecting the fetus by preserving the components and functions of HDL and require further investigation.
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spelling pubmed-75044412020-09-24 Gestational Diabetes Type 2: Variation in High-Density Lipoproteins Composition and Function Pasternak, Yael Biron-Shental, Tal Ohana, Meital Einbinder, Yael Arbib, Nissim Benchetrit, Sydney Zitman-Gal, Tali Int J Mol Sci Article Aims: Class A2 gestational diabetes mellitus (GDMA2) has short- and long-term effects on the mother and child. These may include abnormalities of placentation, damage to endothelial cells and cardiovascular disease. This research investigated the function and composition of high-density lipoproteins (HDL) among women with GDMA2 and their fetuses. Methods: Thirty pregnant women were recruited during admission for delivery. The function and expression of HDL, paraoxonase1 (PON1) and apolipoprotein A1 (APOA1) in the blood samples and the placental tissue were evaluated. The effect of HDL on migration of endothelial cells was measured in vitro. Results: Compared to normal pregnancy (NP), APOA1 in the maternal plasma of women with GDMA2 was decreased. More APOA1 and PON1 were released from HDL of women with GDMA2, compared to NP. Placental APOA1 and PON1 were decreased in GDMA2. For endothelial cells stimulated with TNFα, HDL cell migration was decreased when cells were evaluated with NP-HDL, as compared to GDMA2-HDL. Conclusions: GDMA2 affects the composition and function of HDL in plasma. Changes in HDL commonly seen in GDMA2 were observed in maternal and placental samples, but not in cord samples. These results might indicate a placental role in protecting the fetus by preserving the components and functions of HDL and require further investigation. MDPI 2020-08-30 /pmc/articles/PMC7504441/ /pubmed/32872663 http://dx.doi.org/10.3390/ijms21176281 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pasternak, Yael
Biron-Shental, Tal
Ohana, Meital
Einbinder, Yael
Arbib, Nissim
Benchetrit, Sydney
Zitman-Gal, Tali
Gestational Diabetes Type 2: Variation in High-Density Lipoproteins Composition and Function
title Gestational Diabetes Type 2: Variation in High-Density Lipoproteins Composition and Function
title_full Gestational Diabetes Type 2: Variation in High-Density Lipoproteins Composition and Function
title_fullStr Gestational Diabetes Type 2: Variation in High-Density Lipoproteins Composition and Function
title_full_unstemmed Gestational Diabetes Type 2: Variation in High-Density Lipoproteins Composition and Function
title_short Gestational Diabetes Type 2: Variation in High-Density Lipoproteins Composition and Function
title_sort gestational diabetes type 2: variation in high-density lipoproteins composition and function
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504441/
https://www.ncbi.nlm.nih.gov/pubmed/32872663
http://dx.doi.org/10.3390/ijms21176281
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