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Potential Anti-Diabetic Activity of Pueraria lobata Flower (Flos Puerariae) Extracts

Pueraria lobata (Wild.) Ohwi. (P. lobata) flowers known as ‘Kudzu flower’ contain isoflavonoids and essential oil components. They have a wide range of biological and pharmacological activities, including protective effects against non-alcoholic fatty liver disease, hyperglycemia, and hypolipidemia,...

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Autores principales: Lertpatipanpong, Pattawika, Janpaijit, Sakawrat, Park, Eul-Yong, Kim, Chong-Tai, Baek, Seung Joon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504745/
https://www.ncbi.nlm.nih.gov/pubmed/32878147
http://dx.doi.org/10.3390/molecules25173970
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author Lertpatipanpong, Pattawika
Janpaijit, Sakawrat
Park, Eul-Yong
Kim, Chong-Tai
Baek, Seung Joon
author_facet Lertpatipanpong, Pattawika
Janpaijit, Sakawrat
Park, Eul-Yong
Kim, Chong-Tai
Baek, Seung Joon
author_sort Lertpatipanpong, Pattawika
collection PubMed
description Pueraria lobata (Wild.) Ohwi. (P. lobata) flowers known as ‘Kudzu flower’ contain isoflavonoids and essential oil components. They have a wide range of biological and pharmacological activities, including protective effects against non-alcoholic fatty liver disease, hyperglycemia, and hypolipidemia, anti-mutagenic effects, and benefits for weight loss. However, the molecular mechanism of these effects remains unclear. Our study aimed to systematically examine the effects of flos puerariae crude extract (FPE) as an anti-diabetic agent using in vitro assays. The cytotoxicity of FPE was evaluated using MTS assay in L6 rat myocyte and 3T3-L1 murine fibroblast cell lines. PPARγ binding activity and adipogenesis were examined using dual-luciferase and differentiation assays, respectively. For investigating the anti-diabetic activity, glucose utilization, including GLUT4 protein expression, glucose uptake assay, and GLUT4 translocation using immunofluorescence microscopy were conducted in L6 cells. Furthermore, we assessed the antioxidant and anti-inflammatory activities of FPE. Our results demonstrated the ability to augment glucose uptake in L6 cells and enhance glucose utilization activity by increasing the expression of glucose transporter type 4 (GLUT4). In summary, our findings suggest that FPE may be a potential anti-diabetic substance for the treatment of diabetic patients and can prevent inflammatory or oxidation-related diseases.
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spelling pubmed-75047452020-09-26 Potential Anti-Diabetic Activity of Pueraria lobata Flower (Flos Puerariae) Extracts Lertpatipanpong, Pattawika Janpaijit, Sakawrat Park, Eul-Yong Kim, Chong-Tai Baek, Seung Joon Molecules Article Pueraria lobata (Wild.) Ohwi. (P. lobata) flowers known as ‘Kudzu flower’ contain isoflavonoids and essential oil components. They have a wide range of biological and pharmacological activities, including protective effects against non-alcoholic fatty liver disease, hyperglycemia, and hypolipidemia, anti-mutagenic effects, and benefits for weight loss. However, the molecular mechanism of these effects remains unclear. Our study aimed to systematically examine the effects of flos puerariae crude extract (FPE) as an anti-diabetic agent using in vitro assays. The cytotoxicity of FPE was evaluated using MTS assay in L6 rat myocyte and 3T3-L1 murine fibroblast cell lines. PPARγ binding activity and adipogenesis were examined using dual-luciferase and differentiation assays, respectively. For investigating the anti-diabetic activity, glucose utilization, including GLUT4 protein expression, glucose uptake assay, and GLUT4 translocation using immunofluorescence microscopy were conducted in L6 cells. Furthermore, we assessed the antioxidant and anti-inflammatory activities of FPE. Our results demonstrated the ability to augment glucose uptake in L6 cells and enhance glucose utilization activity by increasing the expression of glucose transporter type 4 (GLUT4). In summary, our findings suggest that FPE may be a potential anti-diabetic substance for the treatment of diabetic patients and can prevent inflammatory or oxidation-related diseases. MDPI 2020-08-31 /pmc/articles/PMC7504745/ /pubmed/32878147 http://dx.doi.org/10.3390/molecules25173970 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lertpatipanpong, Pattawika
Janpaijit, Sakawrat
Park, Eul-Yong
Kim, Chong-Tai
Baek, Seung Joon
Potential Anti-Diabetic Activity of Pueraria lobata Flower (Flos Puerariae) Extracts
title Potential Anti-Diabetic Activity of Pueraria lobata Flower (Flos Puerariae) Extracts
title_full Potential Anti-Diabetic Activity of Pueraria lobata Flower (Flos Puerariae) Extracts
title_fullStr Potential Anti-Diabetic Activity of Pueraria lobata Flower (Flos Puerariae) Extracts
title_full_unstemmed Potential Anti-Diabetic Activity of Pueraria lobata Flower (Flos Puerariae) Extracts
title_short Potential Anti-Diabetic Activity of Pueraria lobata Flower (Flos Puerariae) Extracts
title_sort potential anti-diabetic activity of pueraria lobata flower (flos puerariae) extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504745/
https://www.ncbi.nlm.nih.gov/pubmed/32878147
http://dx.doi.org/10.3390/molecules25173970
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