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Quercitrin Stimulates Hair Growth with Enhanced Expression of Growth Factors via Activation of MAPK/CREB Signaling Pathway

The present study aimed to investigate the molecular mechanism of quercitrin, a major constituent of Hottuynia cordata extract, for its hair growth stimulating activities in cultured human dermal papilla cells (hDPCs). Quercitrin enhanced the cell viability and cellular energy metabolism in cultured...

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Autores principales: Kim, Jaeyoon, Kim, Soon Re, Choi, Yun-Ho, Shin, Jae young, Kim, Chang Deok, Kang, Nae-Gyu, Park, Byung Cheol, Lee, Sanghwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504764/
https://www.ncbi.nlm.nih.gov/pubmed/32887384
http://dx.doi.org/10.3390/molecules25174004
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author Kim, Jaeyoon
Kim, Soon Re
Choi, Yun-Ho
Shin, Jae young
Kim, Chang Deok
Kang, Nae-Gyu
Park, Byung Cheol
Lee, Sanghwa
author_facet Kim, Jaeyoon
Kim, Soon Re
Choi, Yun-Ho
Shin, Jae young
Kim, Chang Deok
Kang, Nae-Gyu
Park, Byung Cheol
Lee, Sanghwa
author_sort Kim, Jaeyoon
collection PubMed
description The present study aimed to investigate the molecular mechanism of quercitrin, a major constituent of Hottuynia cordata extract, for its hair growth stimulating activities in cultured human dermal papilla cells (hDPCs). Quercitrin enhanced the cell viability and cellular energy metabolism in cultured hDPCs by stimulating the production of NAD(P)H and mitochondrial membrane potential (ΔΨ). The expression of Bcl2, an essential marker for anagen hair follicle and cell survival, was increased by quercitrin treatment. Quercitrin also increased the cell proliferation marker Ki67. The expression of growth factors—such as bFGF, KGF, PDGF-AA, and VEGF—were increased by quercitrin both in mRNA and protein levels. In addition, quercitrin was found to increase the phosphorylation of Akt, Erk, and CREB in cultured hDPCs, while inhibitors of MAPKs reversed the effects of quercitrin. Finally, quercitrin stimulated hair shaft growth in cultured human hair follicles. Our data obtained from present study are in line with those previously reported and demonstrate that quercitrin is (one of) the active compound(s) of Hottuynia cordata extract which showed hair growth promoting effects. It is strongly suggested that the hair growth stimulating activity of quercitrin was exerted by enhancing the cellular energy metabolism, increasing the production of growth factors via activation of MAPK/CREB signaling pathway.
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spelling pubmed-75047642020-09-26 Quercitrin Stimulates Hair Growth with Enhanced Expression of Growth Factors via Activation of MAPK/CREB Signaling Pathway Kim, Jaeyoon Kim, Soon Re Choi, Yun-Ho Shin, Jae young Kim, Chang Deok Kang, Nae-Gyu Park, Byung Cheol Lee, Sanghwa Molecules Article The present study aimed to investigate the molecular mechanism of quercitrin, a major constituent of Hottuynia cordata extract, for its hair growth stimulating activities in cultured human dermal papilla cells (hDPCs). Quercitrin enhanced the cell viability and cellular energy metabolism in cultured hDPCs by stimulating the production of NAD(P)H and mitochondrial membrane potential (ΔΨ). The expression of Bcl2, an essential marker for anagen hair follicle and cell survival, was increased by quercitrin treatment. Quercitrin also increased the cell proliferation marker Ki67. The expression of growth factors—such as bFGF, KGF, PDGF-AA, and VEGF—were increased by quercitrin both in mRNA and protein levels. In addition, quercitrin was found to increase the phosphorylation of Akt, Erk, and CREB in cultured hDPCs, while inhibitors of MAPKs reversed the effects of quercitrin. Finally, quercitrin stimulated hair shaft growth in cultured human hair follicles. Our data obtained from present study are in line with those previously reported and demonstrate that quercitrin is (one of) the active compound(s) of Hottuynia cordata extract which showed hair growth promoting effects. It is strongly suggested that the hair growth stimulating activity of quercitrin was exerted by enhancing the cellular energy metabolism, increasing the production of growth factors via activation of MAPK/CREB signaling pathway. MDPI 2020-09-02 /pmc/articles/PMC7504764/ /pubmed/32887384 http://dx.doi.org/10.3390/molecules25174004 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Jaeyoon
Kim, Soon Re
Choi, Yun-Ho
Shin, Jae young
Kim, Chang Deok
Kang, Nae-Gyu
Park, Byung Cheol
Lee, Sanghwa
Quercitrin Stimulates Hair Growth with Enhanced Expression of Growth Factors via Activation of MAPK/CREB Signaling Pathway
title Quercitrin Stimulates Hair Growth with Enhanced Expression of Growth Factors via Activation of MAPK/CREB Signaling Pathway
title_full Quercitrin Stimulates Hair Growth with Enhanced Expression of Growth Factors via Activation of MAPK/CREB Signaling Pathway
title_fullStr Quercitrin Stimulates Hair Growth with Enhanced Expression of Growth Factors via Activation of MAPK/CREB Signaling Pathway
title_full_unstemmed Quercitrin Stimulates Hair Growth with Enhanced Expression of Growth Factors via Activation of MAPK/CREB Signaling Pathway
title_short Quercitrin Stimulates Hair Growth with Enhanced Expression of Growth Factors via Activation of MAPK/CREB Signaling Pathway
title_sort quercitrin stimulates hair growth with enhanced expression of growth factors via activation of mapk/creb signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504764/
https://www.ncbi.nlm.nih.gov/pubmed/32887384
http://dx.doi.org/10.3390/molecules25174004
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