Efficient One-Step Knockout by Electroporation of Ribonucleoproteins Into Zona-Intact Bovine Embryos

Somatic cell nuclear transfer or cytoplasm microinjection have been used to generate genome-edited farm animals; however, these methods have several drawbacks that reduce their efficiency. This study aimed to develop electroporation conditions that allow delivery of CRISPR/Cas9 system to bovine zygo...

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Detalles Bibliográficos
Autores principales: Camargo, Luiz Sergio Almeida, Owen, Joseph R., Van Eenennaam, Alison L., Ross, Pablo Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504904/
https://www.ncbi.nlm.nih.gov/pubmed/33133156
http://dx.doi.org/10.3389/fgene.2020.570069

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504904/
https://www.ncbi.nlm.nih.gov/pubmed/33133156
http://dx.doi.org/10.3389/fgene.2020.570069

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