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Comparative thermo-stability of two Rift Valley fever virus vaccine candidate CL13T with a recombinant arMP-12ΔNSm21/384
Rift Valley fever (RVF) is a zoonotic, viral disease, transmitted by mosquitoes, characterized by high mortality rates in young animals. RVF is an endemic and enzootic disease in the Arabian Peninsula and Africa, causing public health and economic instability. Therefore, it is important to develop v...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Biomedical Informatics
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7505246/ https://www.ncbi.nlm.nih.gov/pubmed/32994680 http://dx.doi.org/10.6026/97320630016547 |
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author | Daouam, S Boumart, Z Elarkam, A Hamdi, J Tadlaoui, KO Ennaji, MM harraka, MEL |
author_facet | Daouam, S Boumart, Z Elarkam, A Hamdi, J Tadlaoui, KO Ennaji, MM harraka, MEL |
author_sort | Daouam, S |
collection | PubMed |
description | Rift Valley fever (RVF) is a zoonotic, viral disease, transmitted by mosquitoes, characterized by high mortality rates in young animals. RVF is an endemic and enzootic disease in the Arabian Peninsula and Africa, causing public health and economic instability. Therefore, it is important to develop vaccines to minimize outbreaks and combat the disease. We documented the stability of the thermo-stability of live attenuated RVF CL13T and recombinant arMP-12ΔNSm21/384 vaccine candidates at different temperatures, including these vaccine viruses in liquid and lyophilized form. The study revealed that both CL13T and recombinant arMP-12ΔNSm21/384 strains were stable for more than 18 months at 4°C. We show that at room temperatures (37°C and 45°C) the CL13T was less temperature sensitive than MP-12NSm-del in both lyophilized and liquid form. These findings are useful for the preparation of RVF vaccines that will avoid the need for a cold chain and therefore, will improve the application of the vaccines under field conditions. |
format | Online Article Text |
id | pubmed-7505246 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Biomedical Informatics |
record_format | MEDLINE/PubMed |
spelling | pubmed-75052462020-09-28 Comparative thermo-stability of two Rift Valley fever virus vaccine candidate CL13T with a recombinant arMP-12ΔNSm21/384 Daouam, S Boumart, Z Elarkam, A Hamdi, J Tadlaoui, KO Ennaji, MM harraka, MEL Bioinformation Research-Article Rift Valley fever (RVF) is a zoonotic, viral disease, transmitted by mosquitoes, characterized by high mortality rates in young animals. RVF is an endemic and enzootic disease in the Arabian Peninsula and Africa, causing public health and economic instability. Therefore, it is important to develop vaccines to minimize outbreaks and combat the disease. We documented the stability of the thermo-stability of live attenuated RVF CL13T and recombinant arMP-12ΔNSm21/384 vaccine candidates at different temperatures, including these vaccine viruses in liquid and lyophilized form. The study revealed that both CL13T and recombinant arMP-12ΔNSm21/384 strains were stable for more than 18 months at 4°C. We show that at room temperatures (37°C and 45°C) the CL13T was less temperature sensitive than MP-12NSm-del in both lyophilized and liquid form. These findings are useful for the preparation of RVF vaccines that will avoid the need for a cold chain and therefore, will improve the application of the vaccines under field conditions. Biomedical Informatics 2020-07-31 /pmc/articles/PMC7505246/ /pubmed/32994680 http://dx.doi.org/10.6026/97320630016547 Text en © 2020 Biomedical Informatics http://creativecommons.org/licenses/by/3.0/ This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License. |
spellingShingle | Research-Article Daouam, S Boumart, Z Elarkam, A Hamdi, J Tadlaoui, KO Ennaji, MM harraka, MEL Comparative thermo-stability of two Rift Valley fever virus vaccine candidate CL13T with a recombinant arMP-12ΔNSm21/384 |
title | Comparative thermo-stability of two Rift Valley fever virus vaccine candidate CL13T with a recombinant arMP-12ΔNSm21/384 |
title_full | Comparative thermo-stability of two Rift Valley fever virus vaccine candidate CL13T with a recombinant arMP-12ΔNSm21/384 |
title_fullStr | Comparative thermo-stability of two Rift Valley fever virus vaccine candidate CL13T with a recombinant arMP-12ΔNSm21/384 |
title_full_unstemmed | Comparative thermo-stability of two Rift Valley fever virus vaccine candidate CL13T with a recombinant arMP-12ΔNSm21/384 |
title_short | Comparative thermo-stability of two Rift Valley fever virus vaccine candidate CL13T with a recombinant arMP-12ΔNSm21/384 |
title_sort | comparative thermo-stability of two rift valley fever virus vaccine candidate cl13t with a recombinant armp-12δnsm21/384 |
topic | Research-Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7505246/ https://www.ncbi.nlm.nih.gov/pubmed/32994680 http://dx.doi.org/10.6026/97320630016547 |
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