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Vigilancia nacional de aislamientos clínicos de Enterococcus faecalis resistentes al linezolid portadores del gen optrA en Colombia, 2014-2019

OBJECTIVE. To describe the epidemiological, phenotypical and genetic characteristics of clinical isolates carrying the optrA gene identified in antimicrobial resistance surveillance by the laboratory of the National Institute of Health of Colombia. METHODS. Between October 2014 and February 2019, 25...

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Autores principales: Saavedra, Sandra Yamile, Bernal, Johan Fabian, Montilla-Escudero, Efrain, Torres, German, Rodríguez, Mabel Karina, Hidalgo, Andrea Melissa, Ovalle, María Victoria, Rivera, Sandra, Perez-Gutierrez, Enrique, Duarte, Carolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Organización Panamericana de la Salud 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7505479/
https://www.ncbi.nlm.nih.gov/pubmed/32968369
http://dx.doi.org/10.26633/RPSP.2020.104
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author Saavedra, Sandra Yamile
Bernal, Johan Fabian
Montilla-Escudero, Efrain
Torres, German
Rodríguez, Mabel Karina
Hidalgo, Andrea Melissa
Ovalle, María Victoria
Rivera, Sandra
Perez-Gutierrez, Enrique
Duarte, Carolina
author_facet Saavedra, Sandra Yamile
Bernal, Johan Fabian
Montilla-Escudero, Efrain
Torres, German
Rodríguez, Mabel Karina
Hidalgo, Andrea Melissa
Ovalle, María Victoria
Rivera, Sandra
Perez-Gutierrez, Enrique
Duarte, Carolina
author_sort Saavedra, Sandra Yamile
collection PubMed
description OBJECTIVE. To describe the epidemiological, phenotypical and genetic characteristics of clinical isolates carrying the optrA gene identified in antimicrobial resistance surveillance by the laboratory of the National Institute of Health of Colombia. METHODS. Between October 2014 and February 2019, 25 isolates of Enterococcus spp. resistant to linezolid were received. Antimicrobial identification and sensitivity were determined using Vitek 2 and the minimum inhibitory concentration (MIC) to linezolid was established with E-test. The optrA gene was detected by PCR, and the genetic diversity of optrA-positive isolates was tested with Diversilab(®). Six isolates were selected to perform whole genome sequencing. RESULTS. The optrA gene was confirmed in 23/25 isolates of E. faecalis from seven departments in Colombia. The isolates presented a MIC to linezolid between 8 and >256µg/mL. Typing by Diversilab(®) showed a wide genetic variability. All the isolates analyzed by whole genome sequencing showed the resistance genes fexA, ermB, lsaA, tet(M), tet(L) and dfrG in addition to optrA and were negative for other mechanisms of resistance to linezolid. Three type sequences and three optrA variants were identified: ST16 (optrA-2), ST476 (optrA-5) and ST618 (optrA-6). The genetic environment of the optrA-2 (ST16) isolates presented the impB, fex, optrA segment, associated with plasmid, while in two isolates (optrA-6 and optrA-5) the transferable chromosomal element Tn6674-like was found. CONCLUSION. OptrA-positive clinical isolates present a high genetic diversity, with different optrA clones and variants related to two types of structures and different mobile genetic elements.
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spelling pubmed-75054792020-09-22 Vigilancia nacional de aislamientos clínicos de Enterococcus faecalis resistentes al linezolid portadores del gen optrA en Colombia, 2014-2019 Saavedra, Sandra Yamile Bernal, Johan Fabian Montilla-Escudero, Efrain Torres, German Rodríguez, Mabel Karina Hidalgo, Andrea Melissa Ovalle, María Victoria Rivera, Sandra Perez-Gutierrez, Enrique Duarte, Carolina Rev Panam Salud Publica Investigación Original OBJECTIVE. To describe the epidemiological, phenotypical and genetic characteristics of clinical isolates carrying the optrA gene identified in antimicrobial resistance surveillance by the laboratory of the National Institute of Health of Colombia. METHODS. Between October 2014 and February 2019, 25 isolates of Enterococcus spp. resistant to linezolid were received. Antimicrobial identification and sensitivity were determined using Vitek 2 and the minimum inhibitory concentration (MIC) to linezolid was established with E-test. The optrA gene was detected by PCR, and the genetic diversity of optrA-positive isolates was tested with Diversilab(®). Six isolates were selected to perform whole genome sequencing. RESULTS. The optrA gene was confirmed in 23/25 isolates of E. faecalis from seven departments in Colombia. The isolates presented a MIC to linezolid between 8 and >256µg/mL. Typing by Diversilab(®) showed a wide genetic variability. All the isolates analyzed by whole genome sequencing showed the resistance genes fexA, ermB, lsaA, tet(M), tet(L) and dfrG in addition to optrA and were negative for other mechanisms of resistance to linezolid. Three type sequences and three optrA variants were identified: ST16 (optrA-2), ST476 (optrA-5) and ST618 (optrA-6). The genetic environment of the optrA-2 (ST16) isolates presented the impB, fex, optrA segment, associated with plasmid, while in two isolates (optrA-6 and optrA-5) the transferable chromosomal element Tn6674-like was found. CONCLUSION. OptrA-positive clinical isolates present a high genetic diversity, with different optrA clones and variants related to two types of structures and different mobile genetic elements. Organización Panamericana de la Salud 2020-09-22 /pmc/articles/PMC7505479/ /pubmed/32968369 http://dx.doi.org/10.26633/RPSP.2020.104 Text en https://creativecommons.org/licenses/by-nc-nd/3.0/igo/legalcode Este es un artículo de acceso abierto distribuido bajo los términos de la licencia Creative Commons Attribution-NonCommercial-NoDerivs 3.0 IGO, que permite su uso, distribución y reproducción en cualquier medio, siempre que el trabajo original se cite de la manera adecuada. No se permiten modificaciones a los artículos ni su uso comercial. Al reproducir un artículo no debe haber ningún indicio de que la OPS o el artículo avalan a una organización o un producto específico. El uso del logo de la OPS no está permitido. Esta leyenda debe conservarse, junto con la URL original del artículo.
spellingShingle Investigación Original
Saavedra, Sandra Yamile
Bernal, Johan Fabian
Montilla-Escudero, Efrain
Torres, German
Rodríguez, Mabel Karina
Hidalgo, Andrea Melissa
Ovalle, María Victoria
Rivera, Sandra
Perez-Gutierrez, Enrique
Duarte, Carolina
Vigilancia nacional de aislamientos clínicos de Enterococcus faecalis resistentes al linezolid portadores del gen optrA en Colombia, 2014-2019
title Vigilancia nacional de aislamientos clínicos de Enterococcus faecalis resistentes al linezolid portadores del gen optrA en Colombia, 2014-2019
title_full Vigilancia nacional de aislamientos clínicos de Enterococcus faecalis resistentes al linezolid portadores del gen optrA en Colombia, 2014-2019
title_fullStr Vigilancia nacional de aislamientos clínicos de Enterococcus faecalis resistentes al linezolid portadores del gen optrA en Colombia, 2014-2019
title_full_unstemmed Vigilancia nacional de aislamientos clínicos de Enterococcus faecalis resistentes al linezolid portadores del gen optrA en Colombia, 2014-2019
title_short Vigilancia nacional de aislamientos clínicos de Enterococcus faecalis resistentes al linezolid portadores del gen optrA en Colombia, 2014-2019
title_sort vigilancia nacional de aislamientos clínicos de enterococcus faecalis resistentes al linezolid portadores del gen optra en colombia, 2014-2019
topic Investigación Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7505479/
https://www.ncbi.nlm.nih.gov/pubmed/32968369
http://dx.doi.org/10.26633/RPSP.2020.104
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