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Los patrones electroforéticos de proteínas salivales permiten diferenciar los grupos transandino y cisandino de las especies de Rhodnius de Colombia
INTRODUCTION: Rhodnius (Hemiptera: Reduviidae: Triatominae) species are made up of haematophagous insect vectors for Trypanosoma cruzi (Chagas’ disease aetiological agent) and T. rangeli, an infective parasite that is not pathogenic for vertebrate hosts. The study of their salivary protein diversity...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Nacional de Salud
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7505504/ https://www.ncbi.nlm.nih.gov/pubmed/32673466 http://dx.doi.org/10.7705/biomedica.4992 |
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author | Meneses, Arlid Rodríguez, Cristian Camilo Suárez, Yazmín Carranza, Julio César Vallejo, Gustavo Adolfo |
author_facet | Meneses, Arlid Rodríguez, Cristian Camilo Suárez, Yazmín Carranza, Julio César Vallejo, Gustavo Adolfo |
author_sort | Meneses, Arlid |
collection | PubMed |
description | INTRODUCTION: Rhodnius (Hemiptera: Reduviidae: Triatominae) species are made up of haematophagous insect vectors for Trypanosoma cruzi (Chagas’ disease aetiological agent) and T. rangeli, an infective parasite that is not pathogenic for vertebrate hosts. The study of their salivary protein diversity enables the obtention of characteristic one-dimensional electrophoretic profiles of some triatomine species; however, few reports have dealt with Rhodnius species salivary proteins electrophoretic patterns. OBJECTIVE: To compare R. colombiensis, R. pallescens, R. pictipes, R. prolixus, and R. robustus’ salivary proteins one-dimensional electrophoretic profiles. MATERIALS AND METHODS: SDS-PAGE was used for obtaining electrophoretic profiles of saliva from the species under study. The unweighted pair group method with arithmetic mean (UPGMA) was used for constructing a phenogram. RESULTS: Electrophoretic profiles of soluble saliva had protein bands ranging from 15 to 45 kDa, thereby enabling the five species studied to be differentiated. The phenogram revealed two main groups, one formed by the Pictipes and Prolixus cis-Andean groups and another consisting of the Pallescens trans-Andean group. CONCLUSION: Differences were revealed regarding R. colombiensis, R. pallescens, R. pictipes, R. prolixus, and R. robustus electrophoretic profiles of salivary proteins; their variability facilitated constructing a phenogram which was taxonomically congruent with the groups from the genus Rhodnius. |
format | Online Article Text |
id | pubmed-7505504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Instituto Nacional de Salud |
record_format | MEDLINE/PubMed |
spelling | pubmed-75055042020-09-22 Los patrones electroforéticos de proteínas salivales permiten diferenciar los grupos transandino y cisandino de las especies de Rhodnius de Colombia Meneses, Arlid Rodríguez, Cristian Camilo Suárez, Yazmín Carranza, Julio César Vallejo, Gustavo Adolfo Biomedica Comunicación Breve INTRODUCTION: Rhodnius (Hemiptera: Reduviidae: Triatominae) species are made up of haematophagous insect vectors for Trypanosoma cruzi (Chagas’ disease aetiological agent) and T. rangeli, an infective parasite that is not pathogenic for vertebrate hosts. The study of their salivary protein diversity enables the obtention of characteristic one-dimensional electrophoretic profiles of some triatomine species; however, few reports have dealt with Rhodnius species salivary proteins electrophoretic patterns. OBJECTIVE: To compare R. colombiensis, R. pallescens, R. pictipes, R. prolixus, and R. robustus’ salivary proteins one-dimensional electrophoretic profiles. MATERIALS AND METHODS: SDS-PAGE was used for obtaining electrophoretic profiles of saliva from the species under study. The unweighted pair group method with arithmetic mean (UPGMA) was used for constructing a phenogram. RESULTS: Electrophoretic profiles of soluble saliva had protein bands ranging from 15 to 45 kDa, thereby enabling the five species studied to be differentiated. The phenogram revealed two main groups, one formed by the Pictipes and Prolixus cis-Andean groups and another consisting of the Pallescens trans-Andean group. CONCLUSION: Differences were revealed regarding R. colombiensis, R. pallescens, R. pictipes, R. prolixus, and R. robustus electrophoretic profiles of salivary proteins; their variability facilitated constructing a phenogram which was taxonomically congruent with the groups from the genus Rhodnius. Instituto Nacional de Salud 2020-06-30 /pmc/articles/PMC7505504/ /pubmed/32673466 http://dx.doi.org/10.7705/biomedica.4992 Text en https://creativecommons.org/licenses/by/4.0/ Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons |
spellingShingle | Comunicación Breve Meneses, Arlid Rodríguez, Cristian Camilo Suárez, Yazmín Carranza, Julio César Vallejo, Gustavo Adolfo Los patrones electroforéticos de proteínas salivales permiten diferenciar los grupos transandino y cisandino de las especies de Rhodnius de Colombia |
title | Los patrones electroforéticos de proteínas salivales permiten diferenciar los grupos transandino y cisandino de las especies de Rhodnius de Colombia |
title_full | Los patrones electroforéticos de proteínas salivales permiten diferenciar los grupos transandino y cisandino de las especies de Rhodnius de Colombia |
title_fullStr | Los patrones electroforéticos de proteínas salivales permiten diferenciar los grupos transandino y cisandino de las especies de Rhodnius de Colombia |
title_full_unstemmed | Los patrones electroforéticos de proteínas salivales permiten diferenciar los grupos transandino y cisandino de las especies de Rhodnius de Colombia |
title_short | Los patrones electroforéticos de proteínas salivales permiten diferenciar los grupos transandino y cisandino de las especies de Rhodnius de Colombia |
title_sort | los patrones electroforéticos de proteínas salivales permiten diferenciar los grupos transandino y cisandino de las especies de rhodnius de colombia |
topic | Comunicación Breve |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7505504/ https://www.ncbi.nlm.nih.gov/pubmed/32673466 http://dx.doi.org/10.7705/biomedica.4992 |
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