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Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones

The large catalytic subunit of acetohydroxyacid synthase (AHAS, EC 2.2.1.6) of Thermotoga maritima (TmcAHAS) was prepared in this study. It possesses high specific activity and excellent stability. The protein and a whole cell catalyst overexpressing the protein were applied to the preparation of α-...

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Autores principales: Liang, Yan-Fei, Yan, Le-Tian, Yue, Qiao, Zhao, Ji-Kui, Luo, Cai-Yun, Gao, Feng, Li, Heng, Gao, Wen-Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7505981/
https://www.ncbi.nlm.nih.gov/pubmed/32958806
http://dx.doi.org/10.1038/s41598-020-72416-6
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author Liang, Yan-Fei
Yan, Le-Tian
Yue, Qiao
Zhao, Ji-Kui
Luo, Cai-Yun
Gao, Feng
Li, Heng
Gao, Wen-Yun
author_facet Liang, Yan-Fei
Yan, Le-Tian
Yue, Qiao
Zhao, Ji-Kui
Luo, Cai-Yun
Gao, Feng
Li, Heng
Gao, Wen-Yun
author_sort Liang, Yan-Fei
collection PubMed
description The large catalytic subunit of acetohydroxyacid synthase (AHAS, EC 2.2.1.6) of Thermotoga maritima (TmcAHAS) was prepared in this study. It possesses high specific activity and excellent stability. The protein and a whole cell catalyst overexpressing the protein were applied to the preparation of α-hydroxyketones including acetoin (AC), 3-hydroxy-2-pentanone (HP), and (R)-phenylacetylcarbinol (R-PAC). The results show that AC and HP could be produced in high yields (84% and 62%, respectively), while R-PAC could be synthesized in a high yield (about 78%) with an R/S ratio of 9:1. Therefore, TmcAHAS and the whole cell catalyst overexpressing the protein could be practically useful bio-catalysts in the preparation of α-hydroxyketones including AC, HP, and R-PAC. To the best of our knowledge, this is the first time that bacterial AHAS was used as a catalyst to prepare HP with a good yield, and also the first time that TmcAHAS was employed to synthesize AC and R-PAC.
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spelling pubmed-75059812020-09-22 Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones Liang, Yan-Fei Yan, Le-Tian Yue, Qiao Zhao, Ji-Kui Luo, Cai-Yun Gao, Feng Li, Heng Gao, Wen-Yun Sci Rep Article The large catalytic subunit of acetohydroxyacid synthase (AHAS, EC 2.2.1.6) of Thermotoga maritima (TmcAHAS) was prepared in this study. It possesses high specific activity and excellent stability. The protein and a whole cell catalyst overexpressing the protein were applied to the preparation of α-hydroxyketones including acetoin (AC), 3-hydroxy-2-pentanone (HP), and (R)-phenylacetylcarbinol (R-PAC). The results show that AC and HP could be produced in high yields (84% and 62%, respectively), while R-PAC could be synthesized in a high yield (about 78%) with an R/S ratio of 9:1. Therefore, TmcAHAS and the whole cell catalyst overexpressing the protein could be practically useful bio-catalysts in the preparation of α-hydroxyketones including AC, HP, and R-PAC. To the best of our knowledge, this is the first time that bacterial AHAS was used as a catalyst to prepare HP with a good yield, and also the first time that TmcAHAS was employed to synthesize AC and R-PAC. Nature Publishing Group UK 2020-09-21 /pmc/articles/PMC7505981/ /pubmed/32958806 http://dx.doi.org/10.1038/s41598-020-72416-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liang, Yan-Fei
Yan, Le-Tian
Yue, Qiao
Zhao, Ji-Kui
Luo, Cai-Yun
Gao, Feng
Li, Heng
Gao, Wen-Yun
Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones
title Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones
title_full Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones
title_fullStr Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones
title_full_unstemmed Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones
title_short Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones
title_sort preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of thermotoga maritima and its application in the syntheses of α-hydroxyketones
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7505981/
https://www.ncbi.nlm.nih.gov/pubmed/32958806
http://dx.doi.org/10.1038/s41598-020-72416-6
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