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Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease
AIM: To profile DNA methylation changes of primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC). MATERIALS & METHODS: Patients with: PBC, PSC with inflammatory bowel disease (IBD), PSC without IBD, and age-, sex-matched controls were profiled for methylomes of peripheral bl...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Medicine Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506472/ https://www.ncbi.nlm.nih.gov/pubmed/32914644 http://dx.doi.org/10.2217/epi-2020-0048 |
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author | Moore, Raymond M Sun, Zhifu Juran, Brian D Lazaridis, Konstantinos N |
author_facet | Moore, Raymond M Sun, Zhifu Juran, Brian D Lazaridis, Konstantinos N |
author_sort | Moore, Raymond M |
collection | PubMed |
description | AIM: To profile DNA methylation changes of primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC). MATERIALS & METHODS: Patients with: PBC, PSC with inflammatory bowel disease (IBD), PSC without IBD, and age-, sex-matched controls were profiled for methylomes of peripheral blood by reduced representation bisulfite sequencing. Differentially methylated CpG (DMC) and differentially methylated region (DMR) were detected and compared. RESULTS: We identified consistently altered DMCs and DMRs across diseases with involvement in key pathways. Many similarities noted between two subtypes of PSC, interestingly few existed between PBC and PSC. DMRs were highly enriched with transcription factor binding. Top DMC changes were validated in liver tissue of an independent cohort. CONCLUSION: Methylome profiling provides insights to PBC and PSC. |
format | Online Article Text |
id | pubmed-7506472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Future Medicine Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-75064722020-09-30 Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease Moore, Raymond M Sun, Zhifu Juran, Brian D Lazaridis, Konstantinos N Epigenomics Preliminary Communication AIM: To profile DNA methylation changes of primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC). MATERIALS & METHODS: Patients with: PBC, PSC with inflammatory bowel disease (IBD), PSC without IBD, and age-, sex-matched controls were profiled for methylomes of peripheral blood by reduced representation bisulfite sequencing. Differentially methylated CpG (DMC) and differentially methylated region (DMR) were detected and compared. RESULTS: We identified consistently altered DMCs and DMRs across diseases with involvement in key pathways. Many similarities noted between two subtypes of PSC, interestingly few existed between PBC and PSC. DMRs were highly enriched with transcription factor binding. Top DMC changes were validated in liver tissue of an independent cohort. CONCLUSION: Methylome profiling provides insights to PBC and PSC. Future Medicine Ltd 2020-09-11 2020-08 /pmc/articles/PMC7506472/ /pubmed/32914644 http://dx.doi.org/10.2217/epi-2020-0048 Text en © 2020 Konstantinos N Lazaridis This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Preliminary Communication Moore, Raymond M Sun, Zhifu Juran, Brian D Lazaridis, Konstantinos N Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease |
title | Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease |
title_full | Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease |
title_fullStr | Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease |
title_full_unstemmed | Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease |
title_short | Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease |
title_sort | genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease |
topic | Preliminary Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506472/ https://www.ncbi.nlm.nih.gov/pubmed/32914644 http://dx.doi.org/10.2217/epi-2020-0048 |
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