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Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease

AIM: To profile DNA methylation changes of primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC). MATERIALS & METHODS: Patients with: PBC, PSC with inflammatory bowel disease (IBD), PSC without IBD, and age-, sex-matched controls were profiled for methylomes of peripheral bl...

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Autores principales: Moore, Raymond M, Sun, Zhifu, Juran, Brian D, Lazaridis, Konstantinos N
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Medicine Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506472/
https://www.ncbi.nlm.nih.gov/pubmed/32914644
http://dx.doi.org/10.2217/epi-2020-0048
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author Moore, Raymond M
Sun, Zhifu
Juran, Brian D
Lazaridis, Konstantinos N
author_facet Moore, Raymond M
Sun, Zhifu
Juran, Brian D
Lazaridis, Konstantinos N
author_sort Moore, Raymond M
collection PubMed
description AIM: To profile DNA methylation changes of primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC). MATERIALS & METHODS: Patients with: PBC, PSC with inflammatory bowel disease (IBD), PSC without IBD, and age-, sex-matched controls were profiled for methylomes of peripheral blood by reduced representation bisulfite sequencing. Differentially methylated CpG (DMC) and differentially methylated region (DMR) were detected and compared. RESULTS: We identified consistently altered DMCs and DMRs across diseases with involvement in key pathways. Many similarities noted between two subtypes of PSC, interestingly few existed between PBC and PSC. DMRs were highly enriched with transcription factor binding. Top DMC changes were validated in liver tissue of an independent cohort. CONCLUSION: Methylome profiling provides insights to PBC and PSC.
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spelling pubmed-75064722020-09-30 Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease Moore, Raymond M Sun, Zhifu Juran, Brian D Lazaridis, Konstantinos N Epigenomics Preliminary Communication AIM: To profile DNA methylation changes of primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC). MATERIALS & METHODS: Patients with: PBC, PSC with inflammatory bowel disease (IBD), PSC without IBD, and age-, sex-matched controls were profiled for methylomes of peripheral blood by reduced representation bisulfite sequencing. Differentially methylated CpG (DMC) and differentially methylated region (DMR) were detected and compared. RESULTS: We identified consistently altered DMCs and DMRs across diseases with involvement in key pathways. Many similarities noted between two subtypes of PSC, interestingly few existed between PBC and PSC. DMRs were highly enriched with transcription factor binding. Top DMC changes were validated in liver tissue of an independent cohort. CONCLUSION: Methylome profiling provides insights to PBC and PSC. Future Medicine Ltd 2020-09-11 2020-08 /pmc/articles/PMC7506472/ /pubmed/32914644 http://dx.doi.org/10.2217/epi-2020-0048 Text en © 2020 Konstantinos N Lazaridis This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Preliminary Communication
Moore, Raymond M
Sun, Zhifu
Juran, Brian D
Lazaridis, Konstantinos N
Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease
title Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease
title_full Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease
title_fullStr Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease
title_full_unstemmed Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease
title_short Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease
title_sort genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease
topic Preliminary Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506472/
https://www.ncbi.nlm.nih.gov/pubmed/32914644
http://dx.doi.org/10.2217/epi-2020-0048
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