Deletion of morpholino binding sites (DeMOBS) to assess specificity of morphant phenotypes

Two complimentary approaches are widely used to study gene function in zebrafish: induction of genetic mutations, usually using targeted nucleases such as CRISPR/Cas9, and suppression of gene expression, typically using Morpholino oligomers. Neither method is perfect. Morpholinos (MOs) sometimes pro...

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Detalles Bibliográficos
Autores principales: Cunningham, Carlee MacPherson, Bellipanni, Gianfranco, Habas, Raymond, Balciunas, Darius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506532/
https://www.ncbi.nlm.nih.gov/pubmed/32958829
http://dx.doi.org/10.1038/s41598-020-71708-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506532/
https://www.ncbi.nlm.nih.gov/pubmed/32958829
http://dx.doi.org/10.1038/s41598-020-71708-1

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