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Development Perspective of Bioelectrocatalysis-Based Biosensors
Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506675/ https://www.ncbi.nlm.nih.gov/pubmed/32858975 http://dx.doi.org/10.3390/s20174826 |
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author | Adachi, Taiki Kitazumi, Yuki Shirai, Osamu Kano, Kenji |
author_facet | Adachi, Taiki Kitazumi, Yuki Shirai, Osamu Kano, Kenji |
author_sort | Adachi, Taiki |
collection | PubMed |
description | Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination. |
format | Online Article Text |
id | pubmed-7506675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75066752020-09-26 Development Perspective of Bioelectrocatalysis-Based Biosensors Adachi, Taiki Kitazumi, Yuki Shirai, Osamu Kano, Kenji Sensors (Basel) Review Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination. MDPI 2020-08-26 /pmc/articles/PMC7506675/ /pubmed/32858975 http://dx.doi.org/10.3390/s20174826 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Adachi, Taiki Kitazumi, Yuki Shirai, Osamu Kano, Kenji Development Perspective of Bioelectrocatalysis-Based Biosensors |
title | Development Perspective of Bioelectrocatalysis-Based Biosensors |
title_full | Development Perspective of Bioelectrocatalysis-Based Biosensors |
title_fullStr | Development Perspective of Bioelectrocatalysis-Based Biosensors |
title_full_unstemmed | Development Perspective of Bioelectrocatalysis-Based Biosensors |
title_short | Development Perspective of Bioelectrocatalysis-Based Biosensors |
title_sort | development perspective of bioelectrocatalysis-based biosensors |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506675/ https://www.ncbi.nlm.nih.gov/pubmed/32858975 http://dx.doi.org/10.3390/s20174826 |
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