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Development Perspective of Bioelectrocatalysis-Based Biosensors

Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and...

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Detalles Bibliográficos
Autores principales: Adachi, Taiki, Kitazumi, Yuki, Shirai, Osamu, Kano, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506675/
https://www.ncbi.nlm.nih.gov/pubmed/32858975
http://dx.doi.org/10.3390/s20174826
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author Adachi, Taiki
Kitazumi, Yuki
Shirai, Osamu
Kano, Kenji
author_facet Adachi, Taiki
Kitazumi, Yuki
Shirai, Osamu
Kano, Kenji
author_sort Adachi, Taiki
collection PubMed
description Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.
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spelling pubmed-75066752020-09-26 Development Perspective of Bioelectrocatalysis-Based Biosensors Adachi, Taiki Kitazumi, Yuki Shirai, Osamu Kano, Kenji Sensors (Basel) Review Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination. MDPI 2020-08-26 /pmc/articles/PMC7506675/ /pubmed/32858975 http://dx.doi.org/10.3390/s20174826 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Adachi, Taiki
Kitazumi, Yuki
Shirai, Osamu
Kano, Kenji
Development Perspective of Bioelectrocatalysis-Based Biosensors
title Development Perspective of Bioelectrocatalysis-Based Biosensors
title_full Development Perspective of Bioelectrocatalysis-Based Biosensors
title_fullStr Development Perspective of Bioelectrocatalysis-Based Biosensors
title_full_unstemmed Development Perspective of Bioelectrocatalysis-Based Biosensors
title_short Development Perspective of Bioelectrocatalysis-Based Biosensors
title_sort development perspective of bioelectrocatalysis-based biosensors
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506675/
https://www.ncbi.nlm.nih.gov/pubmed/32858975
http://dx.doi.org/10.3390/s20174826
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