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Impedimetric Determination of Kanamycin in Milk with Aptasensor Based on Carbon Black-Oligolactide Composite

The determination of antibiotics in food is important due to their negative effect on human health related to antimicrobial resistance problem, renal toxicity, and allergic effects. We propose an impedimetric aptasensor for the determination of kanamycin A (KANA), which was assembled on the glassy c...

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Autores principales: Kulikova, Tatiana, Gorbatchuk, Vladimir, Stoikov, Ivan, Rogov, Alexey, Evtugyn, Gennady, Hianik, Tibor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506709/
https://www.ncbi.nlm.nih.gov/pubmed/32839389
http://dx.doi.org/10.3390/s20174738
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author Kulikova, Tatiana
Gorbatchuk, Vladimir
Stoikov, Ivan
Rogov, Alexey
Evtugyn, Gennady
Hianik, Tibor
author_facet Kulikova, Tatiana
Gorbatchuk, Vladimir
Stoikov, Ivan
Rogov, Alexey
Evtugyn, Gennady
Hianik, Tibor
author_sort Kulikova, Tatiana
collection PubMed
description The determination of antibiotics in food is important due to their negative effect on human health related to antimicrobial resistance problem, renal toxicity, and allergic effects. We propose an impedimetric aptasensor for the determination of kanamycin A (KANA), which was assembled on the glassy carbon electrode by the deposition of carbon black in a chitosan matrix followed by carbodiimide binding of aminated aptamer mixed with oligolactide derivative of thiacalix[4]arene in a cone configuration. The assembling was monitored by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. In the presence of the KANA, the charge transfer resistance of the inner interface surprisingly decreased with the analyte concentration within 0.7 and 50 nM (limit of detection 0.3 nM). This was attributed to the partial shielding of the negative charge of the aptamer and of its support, a highly porous 3D structure of the surface layer caused by a macrocyclic core of the carrier. The use of electrostatic assembling in the presence of cationic polyelectrolyte decreased tenfold the detectable concentration of KANA. The aptasensor was successfully tested in the determination of KANA in spiked milk and yogurt with recoveries within 95% and 115%.
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spelling pubmed-75067092020-09-26 Impedimetric Determination of Kanamycin in Milk with Aptasensor Based on Carbon Black-Oligolactide Composite Kulikova, Tatiana Gorbatchuk, Vladimir Stoikov, Ivan Rogov, Alexey Evtugyn, Gennady Hianik, Tibor Sensors (Basel) Article The determination of antibiotics in food is important due to their negative effect on human health related to antimicrobial resistance problem, renal toxicity, and allergic effects. We propose an impedimetric aptasensor for the determination of kanamycin A (KANA), which was assembled on the glassy carbon electrode by the deposition of carbon black in a chitosan matrix followed by carbodiimide binding of aminated aptamer mixed with oligolactide derivative of thiacalix[4]arene in a cone configuration. The assembling was monitored by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. In the presence of the KANA, the charge transfer resistance of the inner interface surprisingly decreased with the analyte concentration within 0.7 and 50 nM (limit of detection 0.3 nM). This was attributed to the partial shielding of the negative charge of the aptamer and of its support, a highly porous 3D structure of the surface layer caused by a macrocyclic core of the carrier. The use of electrostatic assembling in the presence of cationic polyelectrolyte decreased tenfold the detectable concentration of KANA. The aptasensor was successfully tested in the determination of KANA in spiked milk and yogurt with recoveries within 95% and 115%. MDPI 2020-08-21 /pmc/articles/PMC7506709/ /pubmed/32839389 http://dx.doi.org/10.3390/s20174738 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kulikova, Tatiana
Gorbatchuk, Vladimir
Stoikov, Ivan
Rogov, Alexey
Evtugyn, Gennady
Hianik, Tibor
Impedimetric Determination of Kanamycin in Milk with Aptasensor Based on Carbon Black-Oligolactide Composite
title Impedimetric Determination of Kanamycin in Milk with Aptasensor Based on Carbon Black-Oligolactide Composite
title_full Impedimetric Determination of Kanamycin in Milk with Aptasensor Based on Carbon Black-Oligolactide Composite
title_fullStr Impedimetric Determination of Kanamycin in Milk with Aptasensor Based on Carbon Black-Oligolactide Composite
title_full_unstemmed Impedimetric Determination of Kanamycin in Milk with Aptasensor Based on Carbon Black-Oligolactide Composite
title_short Impedimetric Determination of Kanamycin in Milk with Aptasensor Based on Carbon Black-Oligolactide Composite
title_sort impedimetric determination of kanamycin in milk with aptasensor based on carbon black-oligolactide composite
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506709/
https://www.ncbi.nlm.nih.gov/pubmed/32839389
http://dx.doi.org/10.3390/s20174738
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