A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging

The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocy...

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Detalles Bibliográficos
Autores principales: Wang, Zongcheng, Zheng, Huihuang, Zhang, Chengliang, Tang, Dongfang, Wu, Qiyao, Dessie, Wubliker, Jiang, Yuren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506812/
https://www.ncbi.nlm.nih.gov/pubmed/32846934
http://dx.doi.org/10.3390/s20174768
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author Wang, Zongcheng
Zheng, Huihuang
Zhang, Chengliang
Tang, Dongfang
Wu, Qiyao
Dessie, Wubliker
Jiang, Yuren
author_facet Wang, Zongcheng
Zheng, Huihuang
Zhang, Chengliang
Tang, Dongfang
Wu, Qiyao
Dessie, Wubliker
Jiang, Yuren
author_sort Wang, Zongcheng
collection PubMed
description The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound YZ-A4 as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor YZ-A4 to selenocysteine was from 0 to 32 μM, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that YZ-A4 could be used for fluorescence imaging of selenocysteine in living cells.
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spelling pubmed-75068122020-09-26 A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging Wang, Zongcheng Zheng, Huihuang Zhang, Chengliang Tang, Dongfang Wu, Qiyao Dessie, Wubliker Jiang, Yuren Sensors (Basel) Letter The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound YZ-A4 as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor YZ-A4 to selenocysteine was from 0 to 32 μM, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that YZ-A4 could be used for fluorescence imaging of selenocysteine in living cells. MDPI 2020-08-24 /pmc/articles/PMC7506812/ /pubmed/32846934 http://dx.doi.org/10.3390/s20174768 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Letter
Wang, Zongcheng
Zheng, Huihuang
Zhang, Chengliang
Tang, Dongfang
Wu, Qiyao
Dessie, Wubliker
Jiang, Yuren
A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging
title A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging
title_full A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging
title_fullStr A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging
title_full_unstemmed A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging
title_short A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging
title_sort red emissive fluorescent turn-on sensor for the rapid detection of selenocysteine and its application in living cells imaging
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506812/
https://www.ncbi.nlm.nih.gov/pubmed/32846934
http://dx.doi.org/10.3390/s20174768
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