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Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin
Human pluripotent stem cells (hPSCs) are a potent source of clinically relevant mesenchymal stem cells (MSCs) that confer functional and structural benefits in cell therapy and tissue regeneration. Obtaining sufficient numbers of MSCs in a short period of time and enhancing the differentiation poten...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7507081/ https://www.ncbi.nlm.nih.gov/pubmed/32995130 http://dx.doi.org/10.1002/advs.202001365 |
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author | Cha, Byung‐Hyun Kim, Jin‐Su Bello, Alvin Lee, Geun‐Hui Kim, Do‐Hyun Kim, Byoung Ju Arai, Yoshie Choi, Bogyu Park, Hansoo Lee, Soo‐Hong |
author_facet | Cha, Byung‐Hyun Kim, Jin‐Su Bello, Alvin Lee, Geun‐Hui Kim, Do‐Hyun Kim, Byoung Ju Arai, Yoshie Choi, Bogyu Park, Hansoo Lee, Soo‐Hong |
author_sort | Cha, Byung‐Hyun |
collection | PubMed |
description | Human pluripotent stem cells (hPSCs) are a potent source of clinically relevant mesenchymal stem cells (MSCs) that confer functional and structural benefits in cell therapy and tissue regeneration. Obtaining sufficient numbers of MSCs in a short period of time and enhancing the differentiation potential of MSCs can be offered the potential to improve the regenerative activity of MSCs therapy. In addition, the underlying processes in the isolation and derivation of MSCs from hPSCs are still poorly understood and controlled. To overcome these clinical needs, an efficient and simplified technique on the isolation of MSCs from spontaneously differentiated human embryonic stem cells (hESCs) via integrin α5β1 (fibronectin (FN) receptor)‐to‐FN interactions (hESC‐FN‐MSCs) is successfully developed. It is demonstrated that hESC‐FN‐MSCs exhibit a typical MSC surface phenotype, cellular morphology, with the whole transcriptome similar to conventional adult MSCs; but show higher proliferative capacity, more efficient trilineage differentiation, enhanced cytokine secretion, and attenuated cellular senescence. In addition, the therapeutic potential and regenerative capacity of the isolated hESC‐FN‐MSCs are confirmed by in vitro and in vivo multilineage differentiation. This novel method will be useful in the generation of abundant amounts of clinically relevant MSCs for stem cell therapeutics and regenerative medicine. |
format | Online Article Text |
id | pubmed-7507081 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75070812020-09-28 Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin Cha, Byung‐Hyun Kim, Jin‐Su Bello, Alvin Lee, Geun‐Hui Kim, Do‐Hyun Kim, Byoung Ju Arai, Yoshie Choi, Bogyu Park, Hansoo Lee, Soo‐Hong Adv Sci (Weinh) Full Papers Human pluripotent stem cells (hPSCs) are a potent source of clinically relevant mesenchymal stem cells (MSCs) that confer functional and structural benefits in cell therapy and tissue regeneration. Obtaining sufficient numbers of MSCs in a short period of time and enhancing the differentiation potential of MSCs can be offered the potential to improve the regenerative activity of MSCs therapy. In addition, the underlying processes in the isolation and derivation of MSCs from hPSCs are still poorly understood and controlled. To overcome these clinical needs, an efficient and simplified technique on the isolation of MSCs from spontaneously differentiated human embryonic stem cells (hESCs) via integrin α5β1 (fibronectin (FN) receptor)‐to‐FN interactions (hESC‐FN‐MSCs) is successfully developed. It is demonstrated that hESC‐FN‐MSCs exhibit a typical MSC surface phenotype, cellular morphology, with the whole transcriptome similar to conventional adult MSCs; but show higher proliferative capacity, more efficient trilineage differentiation, enhanced cytokine secretion, and attenuated cellular senescence. In addition, the therapeutic potential and regenerative capacity of the isolated hESC‐FN‐MSCs are confirmed by in vitro and in vivo multilineage differentiation. This novel method will be useful in the generation of abundant amounts of clinically relevant MSCs for stem cell therapeutics and regenerative medicine. John Wiley and Sons Inc. 2020-07-19 /pmc/articles/PMC7507081/ /pubmed/32995130 http://dx.doi.org/10.1002/advs.202001365 Text en © 2020 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Cha, Byung‐Hyun Kim, Jin‐Su Bello, Alvin Lee, Geun‐Hui Kim, Do‐Hyun Kim, Byoung Ju Arai, Yoshie Choi, Bogyu Park, Hansoo Lee, Soo‐Hong Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin |
title | Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin |
title_full | Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin |
title_fullStr | Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin |
title_full_unstemmed | Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin |
title_short | Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin |
title_sort | efficient isolation and enrichment of mesenchymal stem cells from human embryonic stem cells by utilizing the interaction between integrin α5β1 and fibronectin |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7507081/ https://www.ncbi.nlm.nih.gov/pubmed/32995130 http://dx.doi.org/10.1002/advs.202001365 |
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