Cargando…

A modified method for enzymatic isolation of and subsequent wax extraction from Arabidopsis thaliana leaf cuticle

BACKGROUND: The plant cuticle represents one of the major adaptations of vascular plants to terrestrial life. Cuticular permeability and chemical composition differ among species. Arabidopsis thaliana is a widely used model for biochemical and molecular genetic studies in plants. However, attempts t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Vráblová, Martina, Vrábl, Daniel, Sokolová, Barbora, Marková, Dominika, Hronková, Marie
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7507672/ https://www.ncbi.nlm.nih.gov/pubmed/32973915 http://dx.doi.org/10.1186/s13007-020-00673-7

Ejemplares similares

Increased Cuticle Waxes by Overexpression of WSD1 Improves Osmotic Stress Tolerance in Arabidopsis thaliana and Camelina sativa
por: Abdullah, Hesham M., et al.
Publicado: (2021)

Chlorophyll a fluorescence, under half of the adaptive growth-irradiance, for high-throughput sensing of leaf-water deficit in Arabidopsis thaliana accessions
por: Mishra, Kumud B., et al.
Publicado: (2016)

The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana
por: Wagner, Lucas, et al.
Publicado: (2017)

Exogenous hormones influence Brassica napus leaf cuticular wax deposition and cuticle function
por: Yuan, Zheng, et al.
Publicado: (2020)

Metabolic profiling of laser microdissected vascular bundles of Arabidopsis thaliana
por: Schad, Martina, et al.
Publicado: (2005)

A device for single leaf labelling with CO(2) isotopes to study carbon allocation and partitioning in Arabidopsis thaliana
por: Kölling, Katharina, et al.
Publicado: (2013)

Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles
por: Seufert, Pascal, et al.
Publicado: (2022)

Methods for grafting Arabidopsis thaliana and Eutrema salsugineum
por: Li, Yan, et al.
Publicado: (2019)

Photolysis of caged cytokinin in single cells of Arabidopsis thaliana
por: Dow, Lachlan, et al.
Publicado: (2022)

Arabidopsis thaliana Cuticle Composition Contributes to Differential Defense Response to Botrytis cinerea
por: Aragón, Wendy, et al.
Publicado: (2021)

Growth and stress response in Arabidopsis thaliana, Nicotiana benthamiana, Glycine max, Solanum tuberosum and Brassica napus cultivated under polychromatic LEDs
por: Janda, Martin, et al.
Publicado: (2015)

An aeroponic culture system for the study of root herbivory on Arabidopsis thaliana
por: Vaughan, Martha M, et al.
Publicado: (2011)

Tender leaf and fully-expanded leaf exhibited distinct cuticle structure and wax lipid composition in Camellia sinensis cv Fuyun 6
por: Zhu, Xiaofang, et al.
Publicado: (2018)

A method for detecting single mRNA molecules in Arabidopsis thaliana
por: Duncan, Susan, et al.
Publicado: (2016)

Cut and paste: temperature-enhanced cotyledon micrografting for Arabidopsis thaliana seedlings
por: Bartusch, Kai, et al.
Publicado: (2020)

Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis
por: Xu, Yangyang, et al.
Publicado: (2016)

Composition of secondary alcohols, ketones, alkanediols, and ketols in Arabidopsis thaliana cuticular waxes
por: Wen, Miao, et al.
Publicado: (2009)

Cuticular Waxes of Arabidopsis thaliana Shoots: Cell-Type-Specific Composition and Biosynthesis
por: Hegebarth, Daniela, et al.
Publicado: (2017)

An efficient flat-surface collar-free grafting method for Arabidopsis thaliana seedlings
por: Marsch-Martínez, Nayelli, et al.
Publicado: (2013)

Image-based methods for phenotyping growth dynamics and fitness components in Arabidopsis thaliana
por: Vasseur, François, et al.
Publicado: (2018)

A customizable method to characterize Arabidopsis thaliana transpiration under drought conditions
por: de Ollas, Carlos, et al.
Publicado: (2019)

Protocol: Streamlined sub-protocols for floral-dip transformation and selection of transformants in Arabidopsis thaliana
por: Davis, Amanda M, et al.
Publicado: (2009)

Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana
por: Wan, Yinglang, et al.
Publicado: (2011)

In Silico Evaluation of Predicted Regulatory Interactions in Arabidopsis thaliana
por: Nero, Damion, et al.
Publicado: (2009)

Expression of ovate family protein 8 affects epicuticular waxes accumulation in Arabidopsis thaliana
por: Tang, Yao, et al.
Publicado: (2018)

Protocol: optimising hydroponic growth systems for nutritional and physiological analysis of Arabidopsis thaliana and other plants
por: Conn, Simon J, et al.
Publicado: (2013)

Chlorophyll fluorescence emission can screen cold tolerance of cold acclimated Arabidopsis thaliana accessions
por: Mishra, Anamika, et al.
Publicado: (2014)

“Rhizoponics”: a novel hydroponic rhizotron for root system analyses on mature Arabidopsis thaliana plants
por: Mathieu, Laura, et al.
Publicado: (2015)

Characterization of highly efficient heavy-ion mutagenesis in Arabidopsis thaliana
por: Kazama, Yusuke, et al.
Publicado: (2011)

A rapid and robust method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation
por: Harrison, Samuel J, et al.
Publicado: (2006)

The Arabidopsis thaliana-Alternaria brassicicola pathosystem: A model interaction for investigating seed transmission of necrotrophic fungi
por: Pochon, Stephanie, et al.
Publicado: (2012)

Overexpressing Arabidopsis thaliana ACBP6 in transgenic rapid-cycling Brassica napus confers cold tolerance
por: Alahakoon, Aruni Y., et al.
Publicado: (2022)

Combined SPRi Sensor for Simultaneous Detection of Nitrate and Ammonium in Wastewater
por: Vráblová, Martina, et al.
Publicado: (2021)

Cuticle structure and chemical composition of waxes in Phaeoceros laevis (L.) Prosk (Notothyladaceae, Anthocerotophyta)
por: Matos, Tamara Machado, et al.
Publicado: (2022)

An easy-to-use primer design tool to address paralogous loci and T-DNA insertion sites in the genome of Arabidopsis thaliana
por: Huep, Gunnar, et al.
Publicado: (2014)

Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana
por: Czarnecki, Olaf, et al.
Publicado: (2016)

Protocol: a medium-throughput method for determination of cellulose content from single stem pieces of Arabidopsis thaliana
por: Kumar, Manoj, et al.
Publicado: (2015)

Asymmetric water transport in dense leaf cuticles and cuticle-inspired compositionally graded membranes
por: Kamtsikakis, Aristotelis, et al.
Publicado: (2021)

Analysis of plant cuticles and their interactions with agrochemical surfactants using a 3D printed diffusion chamber
por: Vittal, Lakshmi Venkatesha Manyu, et al.
Publicado: (2023)

3D fluorescent in situ hybridization using Arabidopsis leaf cryosections and isolated nuclei
por: Tirichine, Leïla, et al.
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_8sr0ne9n51vfb5p52lnttfbet6