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Highly efficient gene transfer in the mouse gut microbiota is enabled by the Incl(2) conjugative plasmid TP114

The gut microbiota is a suspected hotspot for bacterial conjugation due to its high density and diversity of microorganisms. However, the contribution of different conjugative plasmid families to horizontal gene transfer in this environment remains poorly characterized. Here, we systematically quant...

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Autores principales: Neil, Kevin, Allard, Nancy, Grenier, Frédéric, Burrus, Vincent, Rodrigue, Sébastien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508951/
https://www.ncbi.nlm.nih.gov/pubmed/32963323
http://dx.doi.org/10.1038/s42003-020-01253-0
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author Neil, Kevin
Allard, Nancy
Grenier, Frédéric
Burrus, Vincent
Rodrigue, Sébastien
author_facet Neil, Kevin
Allard, Nancy
Grenier, Frédéric
Burrus, Vincent
Rodrigue, Sébastien
author_sort Neil, Kevin
collection PubMed
description The gut microbiota is a suspected hotspot for bacterial conjugation due to its high density and diversity of microorganisms. However, the contribution of different conjugative plasmid families to horizontal gene transfer in this environment remains poorly characterized. Here, we systematically quantified the transfer rates in the mouse intestinal tract for 13 conjugative plasmids encompassing 10 major incompatibility groups. The vast majority of these plasmids were unable to perform conjugation in situ or only reached relatively low transfer rates. Surprisingly, IncI(2) conjugative plasmid TP114 was identified as a proficient DNA delivery system in this environment, with the ability to transfer to virtually 100% of the probed recipient bacteria. We also show that a type IV pilus present in I-complex conjugative plasmids plays a crucial role for the transfer of TP114 in the mouse intestinal microbiota, most likely by contributing to mating pair stabilization. These results provide new insights on the mobility of genes in the gut microbiota and highlights TP114 as a very efficient DNA delivery system of interest for microbiome editing tools.
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spelling pubmed-75089512020-10-08 Highly efficient gene transfer in the mouse gut microbiota is enabled by the Incl(2) conjugative plasmid TP114 Neil, Kevin Allard, Nancy Grenier, Frédéric Burrus, Vincent Rodrigue, Sébastien Commun Biol Article The gut microbiota is a suspected hotspot for bacterial conjugation due to its high density and diversity of microorganisms. However, the contribution of different conjugative plasmid families to horizontal gene transfer in this environment remains poorly characterized. Here, we systematically quantified the transfer rates in the mouse intestinal tract for 13 conjugative plasmids encompassing 10 major incompatibility groups. The vast majority of these plasmids were unable to perform conjugation in situ or only reached relatively low transfer rates. Surprisingly, IncI(2) conjugative plasmid TP114 was identified as a proficient DNA delivery system in this environment, with the ability to transfer to virtually 100% of the probed recipient bacteria. We also show that a type IV pilus present in I-complex conjugative plasmids plays a crucial role for the transfer of TP114 in the mouse intestinal microbiota, most likely by contributing to mating pair stabilization. These results provide new insights on the mobility of genes in the gut microbiota and highlights TP114 as a very efficient DNA delivery system of interest for microbiome editing tools. Nature Publishing Group UK 2020-09-22 /pmc/articles/PMC7508951/ /pubmed/32963323 http://dx.doi.org/10.1038/s42003-020-01253-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Neil, Kevin
Allard, Nancy
Grenier, Frédéric
Burrus, Vincent
Rodrigue, Sébastien
Highly efficient gene transfer in the mouse gut microbiota is enabled by the Incl(2) conjugative plasmid TP114
title Highly efficient gene transfer in the mouse gut microbiota is enabled by the Incl(2) conjugative plasmid TP114
title_full Highly efficient gene transfer in the mouse gut microbiota is enabled by the Incl(2) conjugative plasmid TP114
title_fullStr Highly efficient gene transfer in the mouse gut microbiota is enabled by the Incl(2) conjugative plasmid TP114
title_full_unstemmed Highly efficient gene transfer in the mouse gut microbiota is enabled by the Incl(2) conjugative plasmid TP114
title_short Highly efficient gene transfer in the mouse gut microbiota is enabled by the Incl(2) conjugative plasmid TP114
title_sort highly efficient gene transfer in the mouse gut microbiota is enabled by the incl(2) conjugative plasmid tp114
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508951/
https://www.ncbi.nlm.nih.gov/pubmed/32963323
http://dx.doi.org/10.1038/s42003-020-01253-0
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