Cargando…

Population genetic structure of the great star coral, Montastraea cavernosa, across the Cuban archipelago with comparisons between microsatellite and SNP markers

Coral reef habitats surrounding Cuba include relatively healthy, well-developed shallow and mesophotic (30–150 m) scleractinian communities at the cross-currents of the Tropical Western Atlantic (TWA). However, Cuba’s coral communities are not immune to the declines observed throughout the TWA, and...

Descripción completa

Detalles Bibliográficos
Autores principales: Sturm, Alexis B., Eckert, Ryan J., Méndez, Juliett González, González-Díaz, Patricia, Voss, Joshua D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508986/
https://www.ncbi.nlm.nih.gov/pubmed/32963271
http://dx.doi.org/10.1038/s41598-020-72112-5
_version_ 1783585508377493504
author Sturm, Alexis B.
Eckert, Ryan J.
Méndez, Juliett González
González-Díaz, Patricia
Voss, Joshua D.
author_facet Sturm, Alexis B.
Eckert, Ryan J.
Méndez, Juliett González
González-Díaz, Patricia
Voss, Joshua D.
author_sort Sturm, Alexis B.
collection PubMed
description Coral reef habitats surrounding Cuba include relatively healthy, well-developed shallow and mesophotic (30–150 m) scleractinian communities at the cross-currents of the Tropical Western Atlantic (TWA). However, Cuba’s coral communities are not immune to the declines observed throughout the TWA, and there is limited information available regarding genetic connectivity, diversity, and structure among these populations. This represents an immense gap in our understanding of coral ecology and population dynamics at both local and regional scales. To address this gap, we evaluated the population genetic structure of the coral Montastraea cavernosa across eight reef sites surrounding Cuba. Colonies were genotyped using nine microsatellite markers and > 9,000 single nucleotide polymorphism (SNP) markers generated using the 2bRAD approach to assess fine-scale genetic structure across these sites. Both the microsatellite and SNP analyses identified patterns of genetic differentiation among sample populations. While the microsatellite analyses did not identify significant genetic structure across the seven shallow M. cavernosa sampling sites, the SNP analyses revealed significant pairwise population differentiation, suggesting that differentiation is greater between eastern and western sites. This study provides insight into methodological differences between microsatellite and SNP markers including potential trade-offs between marker-specific biases, sample size, sequencing costs, and the ability to resolve subtle patterns of population genetic structure. Furthermore, this study suggests that locations in western Cuba may play important roles in this species’ regional metapopulation dynamics and therefore may merit incorporation into developing international management efforts in addition to the local management the sites receive.
format Online
Article
Text
id pubmed-7508986
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-75089862020-09-24 Population genetic structure of the great star coral, Montastraea cavernosa, across the Cuban archipelago with comparisons between microsatellite and SNP markers Sturm, Alexis B. Eckert, Ryan J. Méndez, Juliett González González-Díaz, Patricia Voss, Joshua D. Sci Rep Article Coral reef habitats surrounding Cuba include relatively healthy, well-developed shallow and mesophotic (30–150 m) scleractinian communities at the cross-currents of the Tropical Western Atlantic (TWA). However, Cuba’s coral communities are not immune to the declines observed throughout the TWA, and there is limited information available regarding genetic connectivity, diversity, and structure among these populations. This represents an immense gap in our understanding of coral ecology and population dynamics at both local and regional scales. To address this gap, we evaluated the population genetic structure of the coral Montastraea cavernosa across eight reef sites surrounding Cuba. Colonies were genotyped using nine microsatellite markers and > 9,000 single nucleotide polymorphism (SNP) markers generated using the 2bRAD approach to assess fine-scale genetic structure across these sites. Both the microsatellite and SNP analyses identified patterns of genetic differentiation among sample populations. While the microsatellite analyses did not identify significant genetic structure across the seven shallow M. cavernosa sampling sites, the SNP analyses revealed significant pairwise population differentiation, suggesting that differentiation is greater between eastern and western sites. This study provides insight into methodological differences between microsatellite and SNP markers including potential trade-offs between marker-specific biases, sample size, sequencing costs, and the ability to resolve subtle patterns of population genetic structure. Furthermore, this study suggests that locations in western Cuba may play important roles in this species’ regional metapopulation dynamics and therefore may merit incorporation into developing international management efforts in addition to the local management the sites receive. Nature Publishing Group UK 2020-09-22 /pmc/articles/PMC7508986/ /pubmed/32963271 http://dx.doi.org/10.1038/s41598-020-72112-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sturm, Alexis B.
Eckert, Ryan J.
Méndez, Juliett González
González-Díaz, Patricia
Voss, Joshua D.
Population genetic structure of the great star coral, Montastraea cavernosa, across the Cuban archipelago with comparisons between microsatellite and SNP markers
title Population genetic structure of the great star coral, Montastraea cavernosa, across the Cuban archipelago with comparisons between microsatellite and SNP markers
title_full Population genetic structure of the great star coral, Montastraea cavernosa, across the Cuban archipelago with comparisons between microsatellite and SNP markers
title_fullStr Population genetic structure of the great star coral, Montastraea cavernosa, across the Cuban archipelago with comparisons between microsatellite and SNP markers
title_full_unstemmed Population genetic structure of the great star coral, Montastraea cavernosa, across the Cuban archipelago with comparisons between microsatellite and SNP markers
title_short Population genetic structure of the great star coral, Montastraea cavernosa, across the Cuban archipelago with comparisons between microsatellite and SNP markers
title_sort population genetic structure of the great star coral, montastraea cavernosa, across the cuban archipelago with comparisons between microsatellite and snp markers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508986/
https://www.ncbi.nlm.nih.gov/pubmed/32963271
http://dx.doi.org/10.1038/s41598-020-72112-5
work_keys_str_mv AT sturmalexisb populationgeneticstructureofthegreatstarcoralmontastraeacavernosaacrossthecubanarchipelagowithcomparisonsbetweenmicrosatelliteandsnpmarkers
AT eckertryanj populationgeneticstructureofthegreatstarcoralmontastraeacavernosaacrossthecubanarchipelagowithcomparisonsbetweenmicrosatelliteandsnpmarkers
AT mendezjuliettgonzalez populationgeneticstructureofthegreatstarcoralmontastraeacavernosaacrossthecubanarchipelagowithcomparisonsbetweenmicrosatelliteandsnpmarkers
AT gonzalezdiazpatricia populationgeneticstructureofthegreatstarcoralmontastraeacavernosaacrossthecubanarchipelagowithcomparisonsbetweenmicrosatelliteandsnpmarkers
AT vossjoshuad populationgeneticstructureofthegreatstarcoralmontastraeacavernosaacrossthecubanarchipelagowithcomparisonsbetweenmicrosatelliteandsnpmarkers